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Protocols Electrophoresis
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RAUL CUERO (Talk | contribs) (New page: '''Making Gel'''<br> <br> 1% gel by dilution<br> => 0.6g Low melt PCR agarose<br> => 1.5mL 40x TAE<br> => 58.5mL ddH2O ==> 1.5ul Ethidium Bromide<br> <br> '''Running Gel'''<br> <br...) |
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'''[[Running Gel]]'''<br> | '''[[Running Gel]]'''<br> | ||
<br> | <br> | ||
| - | + | 20ul digestion product<br> | |
Add 5ul of 5x loading buffer<br> | Add 5ul of 5x loading buffer<br> | ||
| - | Load | + | Load lane<br> |
| - | + | 10ul ladder marker <br> | |
<br> | <br> | ||
| - | Run gel for | + | Run gel for 30-45 minutes |
<br> | <br> | ||
<br> | <br> | ||
[[PV Protocols | Protocols]] | [[PV Protocols | Protocols]] | ||
Latest revision as of 17:34, 29 October 2008
Making Gel
1% gel by dilution
=> 0.6g Low melt PCR agarose
=> 1.5mL 40x TAE
=> 58.5mL ddH2O
==> 1.5ul Ethidium Bromide
Running Gel
20ul digestion product
Add 5ul of 5x loading buffer
Load lane
10ul ladder marker
Run gel for 30-45 minutes
Protocols
