Rensselaer/22 August 2008

From 2008.igem.org

(Difference between revisions)

Revision as of 19:51, 22 July 2008

9:00 AM Pip, James, Dimitre

To test the different promoters for the project, we decided on using the plasmid pSB1A2 [[1]] which has a RFP (red fluorescent protein) reporter. The first promoter we will test is the iron promoter from the registry [[2]] on pSB1A2.

The tentative schedule is that we will transform E. coli cells with the plasmids that we want to amplify Wednesday evening or Thursday morning. We will then do a restriction digest on the DNA to test the restriction sites on the cells ( SpeI and XbaI.

@@ Line 1: / Line 1: @@
 '''9:00 AM Pip, James, Dimitre'''
-To test the different promoters for the project, we decided on using the plasmid pSB1A2 [[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2]] which has a RFP (red fluorescent protein) reporter. The first promoter we will test is the iron promoter from the registry [[http://partsregistry.org/wiki/index.php?title=Part:BBa_I765000]].
+To test the different promoters for the project, we decided on using the plasmid pSB1A2 [[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2]] which has a RFP (red fluorescent protein) reporter. The first promoter we will test is the iron promoter from the registry [[http://partsregistry.org/wiki/index.php?title=Part:BBa_I765000]] on pSB1A2.
-The tentative schedule is that we will transform ''E. coli'' cells with the plasmids that we want to amplify Wednesday evening or Thursday morning.
+The tentative schedule is that we will transform ''E. coli'' cells with the plasmids that we want to amplify Wednesday evening or Thursday morning. We will then do a restriction digest on the DNA to test the restriction sites on the cells ( SpeI and XbaI.