From 2008.igem.org
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| - | '''9:00 AM Pip, James, Dimitre'''
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| - | To test the different promoters for the project, we decided on using the plasmid pSB1A2 [[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2]] which has a RFP (red fluorescent protein) reporter. The first promoter we will test is the iron promoter from the registry [[http://partsregistry.org/wiki/index.php?title=Part:BBa_I765000]] on pSB1A2.
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| - | The tentative schedule is that we will transform ''E. coli'' cells with the plasmids that we want to amplify Wednesday evening or Thursday morning, and will then do a restriction digest on the DNA to test the restriction sites on the plasmids ( SpeI [[http://openwetware.org/wiki/SpeI]] and XbaI [[http://openwetware.org/wiki/XbaI]] ). After the restriction digest, the size of the fragments should be 1.05 kb and 2.90 kb. We will also run a control without a digest to make sure that the plasmids remain circular.
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Latest revision as of 20:16, 22 July 2008
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