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Rensselaer/22 August 2008
From 2008.igem.org
9:00 AM Pip, James, Dimitre
To test the different promoters for the project, we decided on using the plasmid pSB1A2 [[1]] which has a RFP (red fluorescent protein) reporter. The first promoter we will test is the iron promoter from the registry [[2]] on pSB1A2.
The tentative schedule is that we will transform E. coli cells with the plasmids that we want to amplify Wednesday evening or Thursday morning, and will then do a restriction digest on the DNA to test the restriction sites on the plasmids ( SpeI [[3]] and XbaI [[4]] ). After the restriction digest, the size of the fragments should be 1.05 kb and 2.90 kb. We will also run a control without a digest to make sure that the plasmids remain circular.
