Rensselaer/7 July 2008

From 2008.igem.org

Revision as of 19:49, 7 July 2008 by Devara (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Overviewed procedure for introducing gene of interest:

pick plasmid filter paper - into buffer

primer design - insert restriction site (comparable with plasmid)

PCR (primers, DNA polymerase, dNTP's,buffer)

Digestion w/ restriction enzymes

Ligation (plasmid and insert)

introduce to bacteria (transformation)

potential problem - psuedomonas - how to get plasmid DNA?

perform PCR on solution