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SOB
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(Difference between revisions)
(New page: == Summary == SOB Medium. Used in growing bacteria for preparing chemically competent cells == Ingredients == *0.5% (w/v) yeast extract *2% (w/v) tryptone *10 mM NaCl *2.5 mM KCl *20 mM ...) |
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| Line 14: | Line 14: | ||
*0.584 g NaCl | *0.584 g NaCl | ||
*0.186 g KCl | *0.186 g KCl | ||
| - | * | + | *120.4g MgSO<sub>4</sub> |
''Note:'' Some formulations of SOB use 10 mM MgCl<sub>2</sub> and 10 mM MgSO<sub>4</sub> instead of 20 mM MgSO<sub>4</sub>. | ''Note:'' Some formulations of SOB use 10 mM MgCl<sub>2</sub> and 10 mM MgSO<sub>4</sub> instead of 20 mM MgSO<sub>4</sub>. | ||
| Line 20: | Line 20: | ||
SOB medium is also available dry premixed from Difco, 0443-17. | SOB medium is also available dry premixed from Difco, 0443-17. | ||
| - | Adjust to pH 7. | + | Adjust to pH 6.8-7.0 prior to use. This requires approximately 25 ml of 1M NaOH per liter. |
| + | |||
| + | MgSO<sub>4</sub> should be autoclaved separately and added just before use. | ||
==15/10 medium== | ==15/10 medium== | ||
Revision as of 02:12, 31 May 2008
Contents |
Summary
SOB Medium. Used in growing bacteria for preparing chemically competent cells
Ingredients
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
Per liter:
- 5 g yeast extract
- 20 g tryptone
- 0.584 g NaCl
- 0.186 g KCl
- 120.4g MgSO4
Note: Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4.
SOB medium is also available dry premixed from Difco, 0443-17.
Adjust to pH 6.8-7.0 prior to use. This requires approximately 25 ml of 1M NaOH per liter.
MgSO4 should be autoclaved separately and added just before use.
15/10 medium
Growth of competent TOP10 cells in Example 2 of the Bloom05 patent is performed in 15/10 broth, which is similar to SOB:
- 1.5% yeast extract
- 1% Bacto-Tryptone
- 10mM NaCl
- 2mM KCl
- 10 mM MgCl2
- 10 mM MgSO4
Source
Adapted From:
F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36
Trivia
SOB supposedly stands for super optimal broth.
