User contributions
From 2008.igem.org
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- 04:51, 1 September 2008 (diff | hist) N Virginia/31 August 2008 (New page: *Miniprepped A67-A69 **Concentrations very good: ~200 or more ng/ul (almost suspiciously good) *Cut A67-A69 with S,P for assumed next stage of 3A *Made gel to look at cut fragments tomorro...) (top)
- 04:50, 1 September 2008 (diff | hist) N Virginia/30 August 2008 (New page: *Spun down A67-69 *Froze pellets Back to Notebook) (top)
- 19:23, 31 August 2008 (diff | hist) Team:Virginia/Notebook
- 18:47, 29 August 2008 (diff | hist) N Virginia/29 August 2008 (New page: ==Overnight Results== *All plates (A67-69) had several dozen colonies ==Accomplished== *Made fresh chloramphenicol antibiotic mix *Made small overnight broths of all 3 assemblies [[Team...) (top)
- 18:46, 29 August 2008 (diff | hist) N Virginia/28 August 2008 (New page: *Ligated (3A) parts for A67-69 **Heat inactivated @65C for 10min *Transformed 30ul of cells with 5ul of ligated DNA Back to Notebook) (top)
- 23:32, 27 August 2008 (diff | hist) N Virginia/27 August 2008 (New page: *Doing restrictions for assemblies of (RBS+BP) with a terminator **Using updated protocol RE: heat-inactivation Back to Notebook) (top)
- 06:00, 20 August 2008 (diff | hist) m Virginia/18 August 2008 (top)
- 06:00, 20 August 2008 (diff | hist) N Virginia/19 August 2008 (New page: ==Overnight Results== *Only plates for B1002 and A64 had some (very small) growth ==Accomplished== *Re-plated A61-63 *Made small overnight broth of B1002 and A64 [[Team:Virginia/Notebo...) (top)
- 22:59, 18 August 2008 (diff | hist) N Virginia/18 August 2008 (New page: *Gelled b0015 e0040 i715039 A59 A60 **Look good *Cut out the above and A58-60 *Doing ligations for A61-66)
- 17:13, 15 August 2008 (diff | hist) N Virginia/15 August 2008 (New page: *Gelled A58-A60 and B0034 Back to Notebook) (top)
- 21:22, 14 August 2008 (diff | hist) Virginia/14 August 2008 (top)
- 18:20, 14 August 2008 (diff | hist) N Virginia/14 August 2008 (New page: ==Overnight Results== *A58 re-plate had a few colonies *A59 re-plate had no colonies ==Accomplished== *Made small broth of A58 (2) *Miniprepped A59-A60, B0034 [[Team:Virginia/Notebook|B...)
- 19:45, 13 August 2008 (diff | hist) Virginia/13 August 2008 (top)
- 19:24, 13 August 2008 (diff | hist) m Virginia/13 August 2008
- 19:24, 13 August 2008 (diff | hist) Virginia/13 August 2008
- 18:08, 13 August 2008 (diff | hist) N Virginia/13 August 2008 (New page: *Made small broth of A58-A60 **Not sure about A58 and A59 - few colonies, satellites *Made small broth of re-streaked B0034 **Still not confident in this *Digested I715039 with E,S and B1...)
- 20:13, 12 August 2008 (diff | hist) N Virginia/12 August 2008 (New page: *Started butanonlysis **Leaving it overnight to make sure reaction goes to completion *Induced A51 in broth, hoping to see red *A58-A60 plates **Some have small colonies, we're letting the...) (top)
- 21:27, 11 August 2008 (diff | hist) N Virginia/11 August 2008 (New page: ==Accomplished== *Re-plate B0034 for the 3rd time (from leftover cells from 2nd transformation) *Small broth of A51 (RFP + GFP) so that we can then take the broth to a spectromoter *Digest...) (top)
- 14:50, 9 August 2008 (diff | hist) N Virginia/9 August 2008 (New page: ==Overnight Results== *No growth on plates except for positive control **Weren't really expecting any **B0034 didn't grow either though unfortunately ==Goals== *Re-transform B0034 and pla...) (top)
- 20:29, 8 August 2008 (diff | hist) Virginia/8 August 2008 (→Accomplished) (top)
- 16:42, 8 August 2008 (diff | hist) Virginia/8 August 2008
- 13:53, 8 August 2008 (diff | hist) N Virginia/8 August 2008 (New page: ==Overnight Results== *No colonies on plates. Back to Notebook)
- 13:52, 8 August 2008 (diff | hist) N Virginia/7 August 2008 (New page: ==Accomplished== *Looked at stained gel of 1st order assemblies (47-50). No inserts were visible. This suggests the assemblies are not correct. *Bought XbaI *Cut out B0034 (RBS) out of gel...) (top)
- 22:01, 30 July 2008 (diff | hist) m Virginia/31 July 2008 (top)
- 21:56, 30 July 2008 (diff | hist) m Virginia/31 July 2008
- 21:56, 30 July 2008 (diff | hist) N Virginia/31 July 2008 (New page: ==TODO== *Redo A1 (R0040 + B0034) *Redo A39 (B0034 + BP1) **Redo NanoDrop of A39 **Redo (if NanoDrop is bad) miniprep of A39 *Overnight broth of more E0840 colonies *NanoDrop 3 miniprepped...)
- 21:48, 30 July 2008 (diff | hist) m Virginia/30 July 2008 (top)
- 20:35, 30 July 2008 (diff | hist) N Virginia/30 July 2008 (New page: ==Overnight Results== *No colonies ==Accomplished== *A42-A45 **Three RBS+BP assemblies have terminator attached **A45 is E0040 + B0015)
- 14:17, 30 July 2008 (diff | hist) Virginia/29 July 2008 (top)
- 18:32, 29 July 2008 (diff | hist) m Team:Virginia/Sandbox
- 18:17, 29 July 2008 (diff | hist) m Team:Virginia/Sandbox
- 18:06, 29 July 2008 (diff | hist) m Team:Virginia/Sandbox
- 18:05, 29 July 2008 (diff | hist) m Team:Virginia/Sandbox
- 18:03, 29 July 2008 (diff | hist) N Team:Virginia/Sandbox (New page: 500px <br> *Home *Team *Project *Modeling *Parts *Notebook)
- 14:57, 29 July 2008 (diff | hist) N Virginia/29 July 2008 (New page: ==Overnight Results== *Although plates have only grown ~13h most have colonies **A37 and A38 don't have colonies. They both used 1AK3)
- 20:17, 28 July 2008 (diff | hist) N Virginia/28 July 2008 (New page: ==Accomplished== *Assemblies: 32-41 **Putting constitutive promoters on BP assemblies (A32-A34) **I715039 + E0840 (A36) **RBS + A29 (A37) **A1 + E0040 (A38) **RBS + BP1-3 (A39-A41) *Transf...) (top)
- 19:14, 25 July 2008 (diff | hist) N Virginia/25 July 2008 (New page: ==Overnight Results== *EVERYTHING WORKED! all ligations were successful thanks to the new T4 ligase *Also the E0840 plate from the day before now had colonies ==Accomplished== *Made smal...) (top)
- 19:35, 24 July 2008 (diff | hist) N Virginia/23 July 2008 (New page: ==Accomplished== *Eluted parts **E0840 **I732097 **E1010 *Digestion - only 3h instead of 4h. Don't want non-specific restriction. **A1 **A25-A30 *Gelled all restriction digests **Cut some ...) (top)
- 19:23, 24 July 2008 (diff | hist) N Virginia/22 July 2008 (New page: ==Accomplished== *SDS-PAGE gel of induced BP1 expression **No apparent new protein expression *Determined new course for assemblies *Sent construction site sequences to be ordered [[Team...) (top)
- 15:48, 24 July 2008 (diff | hist) m Virginia/24 July 2008 (top)
- 15:48, 24 July 2008 (diff | hist) m Virginia/24 July 2008
- 15:47, 24 July 2008 (diff | hist) N Virginia/24 July 2008 (New page: ==Overnight Results== *Parts from distribution did not grow - no colonies ==Goals== *Do assemblies with new T4 DNA ligase **20 ul mix *Transform and plate assemblies *Order Placeholder si...)
- 18:33, 23 July 2008 (diff | hist) Team:Virginia/Protocols
- 13:19, 21 July 2008 (diff | hist) N Virginia/19 July 2008 (New page: *Spun down pellets of overnight broths *Plates with A14 - A17 didn't have results)
- 16:04, 16 July 2008 (diff | hist) m Virginia/16 July 2008 (→Goals) (top)
- 15:12, 16 July 2008 (diff | hist) N Virginia/16 July 2008 (New page: ==Overnight Results== *Plates didn't have anything on them. We plated them late (8pm) so we'll let them keep growing <br> ==Goals== *Run gel of pSB1A10, A3, A$, BP2, BP3 *Redo assembly of:...)
- 20:26, 10 July 2008 (diff | hist) m Virginia/10 July 2008 (top)
- 20:25, 10 July 2008 (diff | hist) N Virginia/10 July 2008 (New page: ==Overnight Results== *Assembly 4 (RBS + BP1) had colonies *Assembly 3 (I715039 + E0240) didn't have colonies ==Goals== *Make small (10ml) broth of colonies in Assembly 4 plate *Redo dige...)
- 17:07, 9 July 2008 (diff | hist) Virginia/9 July 2008 (top)
- 13:59, 9 July 2008 (diff | hist) N Virginia/9 July 2008 (New page: ==Overnight Results== *pSB1A10 screening plasmid grew just fine *Assembly 1 (BP1 + Term) grew more colonies *pSB1AC3 and pSB1AK3 both grew a small number of colonies <br>)
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