User contributions
From 2008.igem.org
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- 18:53, 29 October 2008 (diff | hist) Team:Prairie View/Notebook
- 18:52, 29 October 2008 (diff | hist) Team:Prairie View/Notebook
- 18:51, 29 October 2008 (diff | hist) Team:Prairie View/Notebook
- 18:51, 29 October 2008 (diff | hist) Team:Prairie View/Notebook
- 18:50, 29 October 2008 (diff | hist) Team:Prairie View/Project
- 18:48, 29 October 2008 (diff | hist) Team:Prairie View/Project
- 18:47, 29 October 2008 (diff | hist) Team:Prairie View/Project
- 17:55, 29 October 2008 (diff | hist) PV Protocols (top)
- 17:49, 29 October 2008 (diff | hist) N Protocols PCR Mutation (New page: Set up two separate primer extension reactions<br> One for each top and bottom primer<br><br> '''Cocktail:''' 40ul H2O <br> 5ul 10X Pfu Buffer <br> 1ul dNTP (10mM) <br> 1ul Pfu Polyme...) (top)
- 17:44, 29 October 2008 (diff | hist) PV Protocols
- 17:43, 29 October 2008 (diff | hist) PV Protocols
- 17:42, 29 October 2008 (diff | hist) Protocols PCR (top)
- 17:39, 29 October 2008 (diff | hist) N Protocols PlasmidExtraction (New page: '''Overnight Cultures'''<br> <br> Flask with 25mL - 50mL media with corresponding antibiotic<br> Pick colony with pipette tip/loop and swirl in media<br> 37*C Shake overnight<br> _____...) (top)
- 17:37, 29 October 2008 (diff | hist) N Protocols E.coliTransformation (New page: '''Cocktail:'''<br> <br> 70 ul d.s.H2O<br> 10 ul Ligation product<br> 100 ul Competent cells<br> ___________________<br> 180 ul Total <br> <br> '''Control:'''<br> <br> 70ul d.s.H2O<...) (top)
- 17:35, 29 October 2008 (diff | hist) N Protocols Ligation (New page: '''Cocktail:'''<br> <br> 5.5ul d.s.H2O <br> 1ul Backbone (keep on ice)<br> 2ul Insert (keep on ice)<br> 1ul Buffer (T4 DNA buffer)<br> 0.5ul Ligase (T4 DNA ligase)<br> <br> '''== Negat...) (top)
- 17:34, 29 October 2008 (diff | hist) N Protocols GelPurification (New page: '''Extraction Procedure'''<br> <br> '''1.''' Cut out DNA fragment<br><br> '''2.''' Add 600ul of Buffer QG<br><br> '''3.''' Incubate at ~50*C until gel has completely dissolved, vo...) (top)
- 17:34, 29 October 2008 (diff | hist) Protocols Electrophoresis (top)
- 17:33, 29 October 2008 (diff | hist) N Protocols Electrophoresis (New page: '''Making Gel'''<br> <br> 1% gel by dilution<br> => 0.6g Low melt PCR agarose<br> => 1.5mL 40x TAE<br> => 58.5mL ddH2O ==> 1.5ul Ethidium Bromide<br> <br> '''Running Gel'''<br> <br...)
- 17:31, 29 October 2008 (diff | hist) N Protocols Test Digestion (New page: '''Cocktail:''' <br> 3 uL DNA<br> 1 uL buffer<br> 0.5 uL enzymes<br> 5 uL H2O<br> _______________<br> 10 uL total volume <br> <br> Incubate at 37 C for 30min.<br> Run Gel. <br><br...) (top)
- 17:30, 29 October 2008 (diff | hist) N Protocols Digestion (New page: '''Cocktail:'''<br> <br> <br> 12ul d.s.H2O<br> 3ul plasmid <br> 2ul Buffer<br> 1.5ul Enzyme I<br> 1.5ul Enzyme II <br> __________________<br> 20ul Total<br> <br> 1 hour inc...) (top)
- 17:29, 29 October 2008 (diff | hist) Protocols ColonyPCR (top)
- 17:28, 29 October 2008 (diff | hist) Protocols ColonyPCR
- 17:28, 29 October 2008 (diff | hist) Protocols PCRPurification (top)
- 17:27, 29 October 2008 (diff | hist) N Protocols ColonyPCR (New page: '''Procedure'''<br> '''1''' Add 5 volumes (~250mL) of Buffer PBI to 1 volume (~50mL) of the PCR sample mix <br> '''2''' Check that the color of the mixture is yellow (similiar to B...)
- 17:26, 29 October 2008 (diff | hist) N Protocols PCRPurification (New page: '''Procedure'''<br> '''1''' Add 5 volumes (~250mL) of Buffer PBI to 1 volume (~50mL) of the PCR sample mix <br> '''2''' Check that the color of the mixture is yellow (similiar to B...)
- 17:25, 29 October 2008 (diff | hist) N Protocols PCR (New page: '''Cocktail:''' 37ul H2O <br> 10ul Phusion Buffer <br> ==>(5x HF buffer) <br> 1ul dNTP (10mM) <br> 0.5ul Phusion <br> 0.5ul of each Primer (100uM) <br> 1ul Template <br> _____________...)
- 17:24, 29 October 2008 (diff | hist) N PV Protocols (New page: '''Protocols''' '''PCR'''<br> '''PCR Purification'''<br> '''Colony PCR'''<br> [[Protocols_Digestion | '''Dige...)
- 17:23, 29 October 2008 (diff | hist) Team:Prairie View/Notebook
- 17:10, 29 October 2008 (diff | hist) Team:Prairie View/Notebook
- 17:08, 29 October 2008 (diff | hist) Team:Prairie View/Notebook
- 17:08, 29 October 2008 (diff | hist) Team:Prairie View/Notebook
- 16:59, 29 October 2008 (diff | hist) N File:Figure1.jpg
- 16:55, 29 October 2008 (diff | hist) Team:Prairie View/Notebook
- 16:53, 29 October 2008 (diff | hist) Team:Prairie View/Notebook
- 16:47, 29 October 2008 (diff | hist) Team:Prairie View/Notebook
- 16:28, 29 October 2008 (diff | hist) Team:Prairie View/Notebook
- 16:26, 29 October 2008 (diff | hist) Team:Prairie View/Modeling
- 16:25, 29 October 2008 (diff | hist) Team:Prairie View/Parts
- 16:24, 29 October 2008 (diff | hist) Team:Prairie View/Parts
- 16:23, 29 October 2008 (diff | hist) Team:Prairie View/Parts
- 16:17, 29 October 2008 (diff | hist) Team:Prairie View/Parts
- 16:14, 29 October 2008 (diff | hist) Team:Prairie View/Parts
- 16:10, 29 October 2008 (diff | hist) Team:Prairie View/Project (→Overall project)
- 16:08, 29 October 2008 (diff | hist) Team:Prairie View/Project
- 23:09, 28 October 2008 (diff | hist) Team:Prairie View/Notebook
- 23:06, 28 October 2008 (diff | hist) Team:Prairie View/Notebook
- 23:06, 28 October 2008 (diff | hist) Team:Prairie View/Notebook
- 23:04, 28 October 2008 (diff | hist) Team:Prairie View/Notebook
- 23:03, 28 October 2008 (diff | hist) Team:Prairie View/Notebook
- 23:01, 28 October 2008 (diff | hist) Team:Prairie View/Notebook
- 22:41, 28 October 2008 (diff | hist) Team:Prairie View/Notebook
- 22:40, 28 October 2008 (diff | hist) Team:Prairie View/Team
- 22:40, 28 October 2008 (diff | hist) Team:Prairie View/Team
- 22:38, 28 October 2008 (diff | hist) Team:Prairie View/Team
- 22:37, 28 October 2008 (diff | hist) Team:Prairie View/Team
- 22:37, 28 October 2008 (diff | hist) Team:Prairie View/Team
- 22:34, 28 October 2008 (diff | hist) Team:Prairie View/Team
- 22:20, 28 October 2008 (diff | hist) Team:Prairie View/Notebook
- 22:15, 28 October 2008 (diff | hist) Team:Prairie View/Team
- 22:15, 28 October 2008 (diff | hist) Team:Prairie View/Team
- 22:14, 28 October 2008 (diff | hist) Team:Prairie View/Team
- 22:13, 28 October 2008 (diff | hist) Team:Prairie View/Team
- 22:12, 28 October 2008 (diff | hist) Team:Prairie View/Team
- 22:11, 28 October 2008 (diff | hist) Team:Prairie View/Team
- 22:10, 28 October 2008 (diff | hist) Team:Prairie View/Team
- 22:10, 28 October 2008 (diff | hist) Team:Prairie View/Team (→Students:)
- 22:08, 28 October 2008 (diff | hist) N File:LAB.jpg (top)
- 22:08, 28 October 2008 (diff | hist) Team:Prairie View
- 22:07, 28 October 2008 (diff | hist) Team:Prairie View/Project
- 22:04, 28 October 2008 (diff | hist) Team:Prairie View/Project
- 22:04, 28 October 2008 (diff | hist) Team:Prairie View/Project
- 21:59, 28 October 2008 (diff | hist) Team:Prairie View/Team (→Students:)
- 21:59, 28 October 2008 (diff | hist) Team:Prairie View/Team (→Students:)
- 21:57, 28 October 2008 (diff | hist) Team:Prairie View
- 21:56, 28 October 2008 (diff | hist) Team:Prairie View
- 21:53, 28 October 2008 (diff | hist) N File:Groupphoto.jpg (top)
- 21:52, 28 October 2008 (diff | hist) Team:Prairie View
- 21:49, 28 October 2008 (diff | hist) Team:Prairie View/Project
- 21:47, 28 October 2008 (diff | hist) Team:Prairie View/Project
- 03:43, 30 September 2008 (diff | hist) Team:Colombia/The Project
- 03:42, 30 September 2008 (diff | hist) Team:Colombia/The Project
- 03:40, 30 September 2008 (diff | hist) Team:Colombia/The Project
- 03:38, 30 September 2008 (diff | hist) Team:Colombia/The Team (top)
- 03:37, 30 September 2008 (diff | hist) Team:Colombia/The Project
- 03:36, 30 September 2008 (diff | hist) Team:Colombia
- 03:35, 30 September 2008 (diff | hist) Team:Colombia
- 03:34, 30 September 2008 (diff | hist) Team:Colombia
- 03:33, 30 September 2008 (diff | hist) Team:Colombia
- 03:32, 30 September 2008 (diff | hist) Team:Colombia
- 03:32, 30 September 2008 (diff | hist) Team:Colombia
- 03:30, 30 September 2008 (diff | hist) Team:Colombia
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