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Team:Paris/Construction
2008-10-30T04:10:38Z
<p>Landrain: /* The constructions we were able to obtain allow us to see the pictures below of the fluorescence of one of the promoter (pFlgA) of the FIFO Flagella: */</p>
<hr />
<div>{{Paris/Menu}}<br />
'''Have a look at our [[Team:Paris/Notebook|notebook]].'''<br />
<br />
=Model constructions: from the modelling to the characterization=<br />
<br />
Our project BacterioClock is based on an oscillating FIFO synchronized at the population level. To obtain and have preliminary results of this system we divided it in three "little" modules as the modeling team exposed them previously.<br />
==The First is to demonstrate the [[Team:Paris/Analysis#FIFO_behaviour|FIFO]] which constitutes our core system==<br />
<br />
A simple manner is to implement two class II promoter followed by fluorescent reporter genes like GFP in a bacteria strain that is able to produce the flagella. <br />
<br />
By this way, we are able to see in single cell the order of the activation of pFliL then pFlgA then pFlhB and then the inactivation in the same order as a FIFO will do.<br />
<br />
These constructions are equivalent to the experiments realized by Uri Alon in the article "Using quantitative Blueprint to reprogram the Dynamics of the Flagella Gene Network" Kalir S, Alon U. Cell 2004.<br />
<br />
A better way to observe the FIFO is to study the system with an inducible regulator of class II gene in a bacteria strain deleted for this gene. We are extremely grateful for Alon U. that send us the inducible gene ''FlhDC'', and ''FliA'' in pBad18 plasmid.<br />
These plasmids with our own araC/pBad-EnvZ* will allowed us to study the system in mutated strains that we have in our lab (ΔFlhD, ΔFliA, ΔFlgM, ΔEnvZ). By cotransforming in mutated strains the inducible regulators of the class II promoters with one of this promoter associated to a fluorescent protein, we could characterize the influence of the master regulators of the flagella on their promoter. The fluorescence is normalized to the OD<sub>600</sub>.<br />
<br>For example below are some constructions to see and induce the FIFO:<br />
<br><br><br><br />
[[Image:Felipe01.jpg|500px|center|thumb|pFliL alone]]<br><br><br><br />
[[Image:Felipe02.jpg|500px|center|thumb|3 promoters of the FIFO in one Cell]]<br><br><br><br />
[[Image:Felipe03.jpg|500px|center|thumb|FIFO and oscillating constructions in a single cell]]<br><br><br><br />
<br />
<br />
To perform such a cotransformation we take care about the ORI of each low copy plasmid which are often incompatible and we made all our final constructions in the pSB4T5 plasmid that care the only one resistance that was not already used and the pSC101 ORI which is compatible with the ColE1.<br />
<br />
We could see the FIFO in a more convenient manner with the assembly of two class II promoters associated to different fluorophores. Then we could observed two kinds of fluorescents in One Cell along time. <br />
One problem that could occur is the interaction between the different fluorescents proteins due to the cross-over of their emission and excitation spectra and moreover in our final system that count three fluorophores.<br />
<br />
[[Image:Spectre.jpg |600px |thumb| emission and excitation spectra of ECFP, YFP and mRFP]]<br />
<br />
<br>The choice of ECFP, YFP and mRFP is based upon this consideration and upon the order of appearing in the FIFO.<br />
Indeed by putting the mRFP in last position, we improve the visualization of the FIFO like the maturation time of the protein is about of one hour.<br />
<br />
We thank a lot Arnau Montagu, advisor of the iGEM Valencia Team. He advised us, after his team had troubleshooting with fluorescence, to take care about ''spurious FRET''. This phenomenon occurs even with non covered fluorescent spectra when the concentration of two fluorophores is sufficient so they are enough closer to induce some FRET. But it could happen at lower concentration than 40 μM. <br><br />
<br>- "DsRed as a Potential FRET Partner with CFP and GFP" Michael G. Erickson, Daniel L. Moon, and David T. Yue Biophysical Journal 2003. <br />
<br>- "A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP<br />
Sensor as an Example" Gerard N. M. van der Krogt, Janneke Ogink, Bas Ponsioen, Kees Jalink PLoS 2008.<br />
<br />
<br><br />
<br />
==[[Team:Paris/Analysis#Oscillations|Oscillating]] module==<br />
Few approaches have been thought in order to create the oscillating module. The pTet/TetR system that allows us to precisely control the inhibition of TetR on the Tet promoter. Another system use the natural inhibition of OmpR and of EnvZ via OmpR. We finally perform this system that we have developed since we know thanks to the modeling team that the oscillations are the only way to see the full development of the FIFO.<br />
<br />
==Synchronization module==<br />
The modeling simulation showed us that to have some oscillations that are difficult to obtain in a simple feedforward loop without the delay introduce by the quorum sensing synchronization, and for us it is more interesting to have the FIFO behavior at the population level. We did not achieved the this module but we started the construction of it and have already some intermediate parts that you will find below. <br />
==The constructions we were able to obtain were characterised by fluorescence microscopy (see examples below):==<br />
[[Image:ParisGfp.jpg]]Green fluorescence<br />
[[Image:ParisRfp.jpg]]Red Fluorescence<br />
[[Image:ParisCfp.jpg]]Cyan Fluorescence<br />
<br />
== Table of the constructions we realized==<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136039 pFliL(RBS WT)-ECFP tripart (LVA+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136037 pFliL(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136012 pFlgA(+ RBS WT) - YFP Tripart (LVA+)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136013 pFlgA(+ RBS WT) - YFP Tripart (LVA-)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136054 pFlgA(+ RBS WT)-YFP tripart (AAV+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136053 pFlgA(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136055 pFlhB(+ RBS WT)-mRFP tripart (+LVA)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136065 pFlhB(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136064 pFliL(+ RBS WT)-EnvZ*]<br />
|align=| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136069 pFliL(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136051 pFlhB(+ RBS WT)-EnvZ*]<br />
|align=| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136070 pFlhB(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136066 araC/pBad-EnvZ*]<br />
|align=| A construction made for characterizing the repression power of the mutated EnvZ on our system<br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136029 pLas - gfp Tripart]<br />
|align=| Construction for characterization of the pLas promoter<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136027 pLas - ECFP]<br />
|align=| The same as before but with another fluorophore<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136024 strongest promoter-rbs-lasR-dble ter]<br />
|align=| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136023 Strong promoter-rbs-lasR-dble ter]<br />
|align=| A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria.<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136044 Strongest RBS (1)- LasR activator (+LVA)]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136048 rbs-lasR + dbl ter]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136052 lasR activator + LVA - Double Terminator]<br />
|align=| Intermediary construction<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136057 RBS lasI - GFP tripart]<br />
|align=| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136056 RBS-lasI - ECFP]<br />
|align=| The same as the previous one but with another fluorophore.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136010 pFliA]<br />
|align=| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.<br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136009 pFliL (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136006 pFlgA (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA..<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br> pFlgB (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136008 pFlhB (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136046 EnvZ*]<br />
|align=| EnvZ is a membrane protein involved into the osmotic response and is interacting with OmpR that will bind the promoter of FlhDC ad will repress it. EnvZ* is a mutated form of the coding sequence, giving a phenotype where pFlhDC is completely repressed. EnvZ has been extracted by PVR for the correct mutant strain.<br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align=| FliA is a sigma factor involved into the regulation of very various genes, the promoter used in the FIFO were selected to be higly responding to FliA. In order to have the FliA gene possibly used as biobrick, we mutated 2 sites (EcoRI and PstI) inside the FliA sequence by assemby PCR. <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" |[[Image:Icon_coding.png]]<br>FlhDC<br />
|align=| FlhDC is the main regulator of the flagella genetic system in E. coli. FlhDC regulates all the promoter of the FIFO.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}<br />
<br />
{{Paris/Navig|Team:Paris/Construction}}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T04:09:10Z
<p>Landrain: /* The constructions we were able to obtain allow us to see the pictures below of the fluorescence of one of the promoter of the FIFO Flagella: */</p>
<hr />
<div>{{Paris/Menu}}<br />
'''Have a look at our [[Team:Paris/Notebook|notebook]].'''<br />
<br />
=Model constructions: from the modelling to the characterization=<br />
<br />
Our project BacterioClock is based on an oscillating FIFO synchronized at the population level. To obtain and have preliminary results of this system we divided it in three "little" modules as the modeling team exposed them previously.<br />
==The First is to demonstrate the [[Team:Paris/Analysis#FIFO_behaviour|FIFO]] which constitutes our core system==<br />
<br />
A simple manner is to implement two class II promotor followed by fluorescent reporter genes like GFP in a bacteria strain that is able to produce the flagella. <br />
<br />
By this way, we are able to see in single cell the order of the activation of pFliL then pFlgA then pFlhB and then the inactivation in the same order as a FIFO will do.<br />
<br />
These constructions are equivalent to the experiments realized by Uri Alon in the article "Using quantitative Blueprint to reprogram the Dynamics of the Flagella Gene Network" Kalir S, Alon U. Cell 2004.<br />
<br />
A better way to observe the FIFO is to study the system with an inducible regulator of class II gene in a bacteria strain deleted for this gene. We are extremely grateful for Alon U. that send us the inducible gene ''FlhDC'', and ''FliA'' in pBad18 plasmid.<br />
These plasmids with our own araC/pBad-EnvZ* will allowed us to study the system in mutated strains that we have in our lab (ΔFlhD, ΔFliA, ΔFlgM, ΔEnvZ). By cotransforming in mutated strains the inducibles regulators of the class II promotors with one of this promotor associated to a fluorescent protein, we could characterize the influence of the master regulators of the flagella on their pormoter. The fluorescence is normalized to the OD<sub>600</sub>.<br />
<br>For example below are some constructions to see and induce the FIFO:<br />
<br><br><br><br />
[[Image:Felipe01.jpg|500px|center|thumb|pFliL alone]]<br><br><br><br />
[[Image:Felipe02.jpg|500px|center|thumb|3 promoters of the FIFO in one Cell]]<br><br><br><br />
[[Image:Felipe03.jpg|500px|center|thumb|FIFO and oscillating constructions in a single cell]]<br><br><br><br />
<br />
<br />
To perform such a cotransformation we take care about the ORI of each low copy plasmid which are often incompatible and we made all our final constructions in the pSB4T5 plasmid that care the only one resistance that was not already used and the pSC101 ORI which is compatible with the ColE1.<br />
<br />
We could see the FIFO in a more convenient manner with the assembly of two class II promotors associated to differents fluorophores. Then we could observed two kinds of fluorescents in One Cell along time. <br />
One problem that could occur is the interaction between the differents fluorescents proteins due to the cross-over of their emission and excitation spectra and moreover in our final system that count three fluorophores.<br />
<br />
[[Image:Spectre.jpg |600px |thumb| emission and excitation spectra of ECFP, YFP and mRFP]]<br />
<br />
<br>The choice of ECFP, YFP and mRFP is based upon this consideration and upon the order of appearing in the FIFO.<br />
Indeed by putting the mRFP in last position, we improve the visualization of the FIFO like the maturation time of the protein is about of one hour.<br />
<br />
We thank a lot Arnau Montagu, advisor of the iGEM Valencia Team. He advised us, after his team had troubleshooting with fluorescence, to take care about ''spurious FRET''. This phenomenon occurs even with non covered fluorescent spectra when the concentration of two fluorophores is sufficient so they are enough closer to induce some FRET. But it could happen at lower concentration than 40 μM. <br><br />
<br>- "DsRed as a Potential FRET Partner with CFP and GFP" Michael G. Erickson, Daniel L. Moon, and David T. Yue Biophysical Journal 2003. <br />
<br>- "A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP<br />
Sensor as an Example" Gerard N. M. van der Krogt, Janneke Ogink, Bas Ponsioen, Kees Jalink PLoS 2008.<br />
<br />
<br><br />
<br />
==[[Team:Paris/Analysis#Oscillations|Oscillating]] module==<br />
Few approaches have been thought in order to create the oscillating module. The pTet/TetR system that allows us to precisely control the inhibition of TetR on the Tet promotor. Another system use the natural inhibition of OmpR and of EnvZ via OmpR. We finally perform this system that we have developed since we know thanks to the modeling team that the oscillations are the only way to see the full development of the FIFO.<br />
<br />
==Synchronization module==<br />
The modeling simulation showed us that to have some oscillations that are difficult to obtain in a simple feedforward loop without the delay introduce by the quorum sensing synchronization, and for us it is more interesting to have the FIFO behavior at the population level. We did not achieved the this module but we started the construction of it and have already some intermediate parts that you will find below. <br />
==The constructions we were able to obtain allow us to see the pictures below of the fluorescence of one of the promoter (pFlgA) of the FIFO Flagella:==<br />
[[Image:ParisGfp.jpg]]Green fluorescence<br />
[[Image:ParisRfp.jpg]]Red Fluorescence<br />
[[Image:ParisCfp.jpg]]Cyan Fluorescence<br />
<br />
== Table of the constructions we realized==<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136039 pFliL(RBS WT)-ECFP tripart (LVA+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136037 pFliL(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136012 pFlgA(+ RBS WT) - YFP Tripart (LVA+)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136013 pFlgA(+ RBS WT) - YFP Tripart (LVA-)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136054 pFlgA(+ RBS WT)-YFP tripart (AAV+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136053 pFlgA(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136055 pFlhB(+ RBS WT)-mRFP tripart (+LVA)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136065 pFlhB(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136064 pFliL(+ RBS WT)-EnvZ*]<br />
|align=| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136069 pFliL(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136051 pFlhB(+ RBS WT)-EnvZ*]<br />
|align=| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136070 pFlhB(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136066 araC/pBad-EnvZ*]<br />
|align=| A construction made for characterizing the repression power of the mutated EnvZ on our system<br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136029 pLas - gfp Tripart]<br />
|align=| Construction for characterization of the pLas promoter<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136027 pLas - ECFP]<br />
|align=| The same as before but with another fluorophore<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136024 strongest promoter-rbs-lasR-dble ter]<br />
|align=| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136023 Strong promoter-rbs-lasR-dble ter]<br />
|align=| A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria.<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136044 Strongest RBS (1)- LasR activator (+LVA)]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136048 rbs-lasR + dbl ter]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136052 lasR activator + LVA - Double Terminator]<br />
|align=| Intermediary construction<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136057 RBS lasI - GFP tripart]<br />
|align=| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136056 RBS-lasI - ECFP]<br />
|align=| The same as the previous one but with another fluorophore.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136010 pFliA]<br />
|align=| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.<br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136009 pFliL (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136006 pFlgA (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA..<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br> pFlgB (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136008 pFlhB (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136046 EnvZ*]<br />
|align=| EnvZ is a membrane protein involved into the osmotic response and is interacting with OmpR that will bind the promoter of FlhDC ad will repress it. EnvZ* is a mutated form of the coding sequence, giving a phenotype where pFlhDC is completely repressed. EnvZ has been extracted by PVR for the correct mutant strain.<br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align=| FliA is a sigma factor involved into the regulation of very various genes, the promoter used in the FIFO were selected to be higly responding to FliA. In order to have the FliA gene possibly used as biobrick, we mutated 2 sites (EcoRI and PstI) inside the FliA sequence by assemby PCR. <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" |[[Image:Icon_coding.png]]<br>FlhDC<br />
|align=| FlhDC is the main regulator of the flagella genetic system in E. coli. FlhDC regulates all the promoter of the FIFO.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}<br />
<br />
{{Paris/Navig|Team:Paris/Construction}}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T04:07:36Z
<p>Landrain: /* Synchronization module */</p>
<hr />
<div>{{Paris/Menu}}<br />
'''Have a look at our [[Team:Paris/Notebook|notebook]].'''<br />
<br />
=Model constructions: from the modelling to the characterization=<br />
<br />
Our project BacterioClock is based on an oscillating FIFO synchronized at the population level. To obtain and have preliminary results of this system we divided it in three "little" modules as the modeling team exposed them previously.<br />
==The First is to demonstrate the [[Team:Paris/Analysis#FIFO_behaviour|FIFO]] which constitutes our core system==<br />
<br />
A simple manner is to implement two class II promotor followed by fluorescent reporter genes like GFP in a bacteria strain that is able to produce the flagella. <br />
<br />
By this way, we are able to see in single cell the order of the activation of pFliL then pFlgA then pFlhB and then the inactivation in the same order as a FIFO will do.<br />
<br />
These constructions are equivalent to the experiments realized by Uri Alon in the article "Using quantitative Blueprint to reprogram the Dynamics of the Flagella Gene Network" Kalir S, Alon U. Cell 2004.<br />
<br />
A better way to observe the FIFO is to study the system with an inducible regulator of class II gene in a bacteria strain deleted for this gene. We are extremely grateful for Alon U. that send us the inducible gene ''FlhDC'', and ''FliA'' in pBad18 plasmid.<br />
These plasmids with our own araC/pBad-EnvZ* will allowed us to study the system in mutated strains that we have in our lab (ΔFlhD, ΔFliA, ΔFlgM, ΔEnvZ). By cotransforming in mutated strains the inducibles regulators of the class II promotors with one of this promotor associated to a fluorescent protein, we could characterize the influence of the master regulators of the flagella on their pormoter. The fluorescence is normalized to the OD<sub>600</sub>.<br />
<br>For example below are some constructions to see and induce the FIFO:<br />
<br><br><br><br />
[[Image:Felipe01.jpg|500px|center|thumb|pFliL alone]]<br><br><br><br />
[[Image:Felipe02.jpg|500px|center|thumb|3 promoters of the FIFO in one Cell]]<br><br><br><br />
[[Image:Felipe03.jpg|500px|center|thumb|FIFO and oscillating constructions in a single cell]]<br><br><br><br />
<br />
<br />
To perform such a cotransformation we take care about the ORI of each low copy plasmid which are often incompatible and we made all our final constructions in the pSB4T5 plasmid that care the only one resistance that was not already used and the pSC101 ORI which is compatible with the ColE1.<br />
<br />
We could see the FIFO in a more convenient manner with the assembly of two class II promotors associated to differents fluorophores. Then we could observed two kinds of fluorescents in One Cell along time. <br />
One problem that could occur is the interaction between the differents fluorescents proteins due to the cross-over of their emission and excitation spectra and moreover in our final system that count three fluorophores.<br />
<br />
[[Image:Spectre.jpg |600px |thumb| emission and excitation spectra of ECFP, YFP and mRFP]]<br />
<br />
<br>The choice of ECFP, YFP and mRFP is based upon this consideration and upon the order of appearing in the FIFO.<br />
Indeed by putting the mRFP in last position, we improve the visualization of the FIFO like the maturation time of the protein is about of one hour.<br />
<br />
We thank a lot Arnau Montagu, advisor of the iGEM Valencia Team. He advised us, after his team had troubleshooting with fluorescence, to take care about ''spurious FRET''. This phenomenon occurs even with non covered fluorescent spectra when the concentration of two fluorophores is sufficient so they are enough closer to induce some FRET. But it could happen at lower concentration than 40 μM. <br><br />
<br>- "DsRed as a Potential FRET Partner with CFP and GFP" Michael G. Erickson, Daniel L. Moon, and David T. Yue Biophysical Journal 2003. <br />
<br>- "A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP<br />
Sensor as an Example" Gerard N. M. van der Krogt, Janneke Ogink, Bas Ponsioen, Kees Jalink PLoS 2008.<br />
<br />
<br><br />
<br />
==[[Team:Paris/Analysis#Oscillations|Oscillating]] module==<br />
Few approaches have been thought in order to create the oscillating module. The pTet/TetR system that allows us to precisely control the inhibition of TetR on the Tet promotor. Another system use the natural inhibition of OmpR and of EnvZ via OmpR. We finally perform this system that we have developed since we know thanks to the modeling team that the oscillations are the only way to see the full development of the FIFO.<br />
<br />
==Synchronization module==<br />
The modeling simulation showed us that to have some oscillations that are difficult to obtain in a simple feedforward loop without the delay introduce by the quorum sensing synchronization, and for us it is more interesting to have the FIFO behavior at the population level. We did not achieved the this module but we started the construction of it and have already some intermediate parts that you will find below. <br />
==The constructions we were able to obtain allow us to see the pictures below of the fluorescence of one of the promoter of the FIFO Flagella:==<br />
[[Image:Paris Gfp.jpg]]<br />
[[Image:Paris Rfp.jpg]]<br />
[[Image:Paris Ecfp.jpg]]<br />
<br />
== Table of the constructions we realized==<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136039 pFliL(RBS WT)-ECFP tripart (LVA+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136037 pFliL(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136012 pFlgA(+ RBS WT) - YFP Tripart (LVA+)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136013 pFlgA(+ RBS WT) - YFP Tripart (LVA-)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136054 pFlgA(+ RBS WT)-YFP tripart (AAV+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136053 pFlgA(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136055 pFlhB(+ RBS WT)-mRFP tripart (+LVA)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136065 pFlhB(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136064 pFliL(+ RBS WT)-EnvZ*]<br />
|align=| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136069 pFliL(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136051 pFlhB(+ RBS WT)-EnvZ*]<br />
|align=| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136070 pFlhB(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136066 araC/pBad-EnvZ*]<br />
|align=| A construction made for characterizing the repression power of the mutated EnvZ on our system<br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136029 pLas - gfp Tripart]<br />
|align=| Construction for characterization of the pLas promoter<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136027 pLas - ECFP]<br />
|align=| The same as before but with another fluorophore<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136024 strongest promoter-rbs-lasR-dble ter]<br />
|align=| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136023 Strong promoter-rbs-lasR-dble ter]<br />
|align=| A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria.<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136044 Strongest RBS (1)- LasR activator (+LVA)]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136048 rbs-lasR + dbl ter]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136052 lasR activator + LVA - Double Terminator]<br />
|align=| Intermediary construction<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136057 RBS lasI - GFP tripart]<br />
|align=| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136056 RBS-lasI - ECFP]<br />
|align=| The same as the previous one but with another fluorophore.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136010 pFliA]<br />
|align=| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.<br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136009 pFliL (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136006 pFlgA (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA..<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br> pFlgB (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136008 pFlhB (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136046 EnvZ*]<br />
|align=| EnvZ is a membrane protein involved into the osmotic response and is interacting with OmpR that will bind the promoter of FlhDC ad will repress it. EnvZ* is a mutated form of the coding sequence, giving a phenotype where pFlhDC is completely repressed. EnvZ has been extracted by PVR for the correct mutant strain.<br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align=| FliA is a sigma factor involved into the regulation of very various genes, the promoter used in the FIFO were selected to be higly responding to FliA. In order to have the FliA gene possibly used as biobrick, we mutated 2 sites (EcoRI and PstI) inside the FliA sequence by assemby PCR. <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" |[[Image:Icon_coding.png]]<br>FlhDC<br />
|align=| FlhDC is the main regulator of the flagella genetic system in E. coli. FlhDC regulates all the promoter of the FIFO.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}<br />
<br />
{{Paris/Navig|Team:Paris/Construction}}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T04:06:22Z
<p>Landrain: /* The First is to demonstrate the FIFO which constitutes our core system */</p>
<hr />
<div>{{Paris/Menu}}<br />
=Model constructions: from the modelling to the characterization=<br />
<br />
Our project BacterioClock is based on an oscillating FIFO synchronized at the population level. To obtain and have preliminary results of this system we divided it in three "little" modules as the modeling team exposed them previously.<br />
==The First is to demonstrate the [[Team:Paris/Analysis#FIFO_behaviour|FIFO]] which constitutes our core system==<br />
<br />
A simple manner is to implement two class II promotor followed by fluorescent reporter genes like GFP in a bacteria strain that is able to produce the flagella. <br />
<br />
By this way, we are able to see in single cell the order of the activation of pFliL then pFlgA then pFlhB and then the inactivation in the same order as a FIFO will do.<br />
<br />
These constructions are equivalent to the experiments realized by Uri Alon in the article "Using quantitative Blueprint to reprogram the Dynamics of the Flagella Gene Network" Kalir S, Alon U. Cell 2004.<br />
<br />
A better way to observe the FIFO is to study the system with an inducible regulator of class II gene in a bacteria strain deleted for this gene. We are extremely grateful for Alon U. that send us the inducible gene ''FlhDC'', and ''FliA'' in pBad18 plasmid.<br />
These plasmids with our own araC/pBad-EnvZ* will allowed us to study the system in mutated strains that we have in our lab (ΔFlhD, ΔFliA, ΔFlgM, ΔEnvZ). By cotransforming in mutated strains the inducibles regulators of the class II promotors with one of this promotor associated to a fluorescent protein, we could characterize the influence of the master regulators of the flagella on their pormoter. The fluorescence is normalized to the OD<sub>600</sub>.<br />
<br>For example below are some constructions to see and induce the FIFO:<br />
<br><br><br><br />
[[Image:Felipe01.jpg|500px|center|thumb|pFliL alone]]<br><br><br><br />
[[Image:Felipe02.jpg|500px|center|thumb|3 promoters of the FIFO in one Cell]]<br><br><br><br />
[[Image:Felipe03.jpg|500px|center|thumb|FIFO and oscillating constructions in a single cell]]<br><br><br><br />
<br />
<br />
To perform such a cotransformation we take care about the ORI of each low copy plasmid which are often incompatible and we made all our final constructions in the pSB4T5 plasmid that care the only one resistance that was not already used and the pSC101 ORI which is compatible with the ColE1.<br />
<br />
We could see the FIFO in a more convenient manner with the assembly of two class II promotors associated to differents fluorophores. Then we could observed two kinds of fluorescents in One Cell along time. <br />
One problem that could occur is the interaction between the differents fluorescents proteins due to the cross-over of their emission and excitation spectra and moreover in our final system that count three fluorophores.<br />
<br />
[[Image:Spectre.jpg |600px |thumb| emission and excitation spectra of ECFP, YFP and mRFP]]<br />
<br />
<br>The choice of ECFP, YFP and mRFP is based upon this consideration and upon the order of appearing in the FIFO.<br />
Indeed by putting the mRFP in last position, we improve the visualization of the FIFO like the maturation time of the protein is about of one hour.<br />
<br />
We thank a lot Arnau Montagu, advisor of the iGEM Valencia Team. He advised us, after his team had troubleshooting with fluorescence, to take care about ''spurious FRET''. This phenomenon occurs even with non covered fluorescent spectra when the concentration of two fluorophores is sufficient so they are enough closer to induce some FRET. But it could happen at lower concentration than 40 μM. <br><br />
<br>- "DsRed as a Potential FRET Partner with CFP and GFP" Michael G. Erickson, Daniel L. Moon, and David T. Yue Biophysical Journal 2003. <br />
<br>- "A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP<br />
Sensor as an Example" Gerard N. M. van der Krogt, Janneke Ogink, Bas Ponsioen, Kees Jalink PLoS 2008.<br />
<br />
<br><br />
<br />
==[[Team:Paris/Analysis#Oscillations|Oscillating]] module==<br />
Few approaches have been thought in order to create the oscillating module. The pTet/TetR system that allows us to precisely control the inhibition of TetR on the Tet promotor. Another system use the natural inhibition of OmpR and of EnvZ via OmpR. We finally perform this system that we have developed since we know thanks to the modeling team that the oscillations are the only way to see the full development of the FIFO.<br />
<br />
==Synchronization module==<br />
The modeling simulation showed us that to have some oscillations that are difficult to obtain in a simple feedforward loop without the delay introduce by the quorum sensing synchronization, and for us it is more interesting to have the FIFO behavior at the population level. We did not achieved the this module but we started the construction of it and have already some intermediate parts that you will find below. <br />
The constructions we were able to obtain allow us to see the pictures below of the fluorescence of one of the promoter of the FIFO Flagella:<br />
<br />
== Table of the constructions we realized==<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136039 pFliL(RBS WT)-ECFP tripart (LVA+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136037 pFliL(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136012 pFlgA(+ RBS WT) - YFP Tripart (LVA+)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136013 pFlgA(+ RBS WT) - YFP Tripart (LVA-)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136054 pFlgA(+ RBS WT)-YFP tripart (AAV+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136053 pFlgA(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136055 pFlhB(+ RBS WT)-mRFP tripart (+LVA)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136065 pFlhB(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136064 pFliL(+ RBS WT)-EnvZ*]<br />
|align=| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136069 pFliL(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136051 pFlhB(+ RBS WT)-EnvZ*]<br />
|align=| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136070 pFlhB(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136066 araC/pBad-EnvZ*]<br />
|align=| A construction made for characterizing the repression power of the mutated EnvZ on our system<br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136029 pLas - gfp Tripart]<br />
|align=| Construction for characterization of the pLas promoter<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136027 pLas - ECFP]<br />
|align=| The same as before but with another fluorophore<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136024 strongest promoter-rbs-lasR-dble ter]<br />
|align=| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136023 Strong promoter-rbs-lasR-dble ter]<br />
|align=| A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria.<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136044 Strongest RBS (1)- LasR activator (+LVA)]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136048 rbs-lasR + dbl ter]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136052 lasR activator + LVA - Double Terminator]<br />
|align=| Intermediary construction<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136057 RBS lasI - GFP tripart]<br />
|align=| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136056 RBS-lasI - ECFP]<br />
|align=| The same as the previous one but with another fluorophore.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136010 pFliA]<br />
|align=| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.<br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136009 pFliL (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136006 pFlgA (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA..<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br> pFlgB (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136008 pFlhB (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136046 EnvZ*]<br />
|align=| EnvZ is a membrane protein involved into the osmotic response and is interacting with OmpR that will bind the promoter of FlhDC ad will repress it. EnvZ* is a mutated form of the coding sequence, giving a phenotype where pFlhDC is completely repressed. EnvZ has been extracted by PVR for the correct mutant strain.<br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align=| FliA is a sigma factor involved into the regulation of very various genes, the promoter used in the FIFO were selected to be higly responding to FliA. In order to have the FliA gene possibly used as biobrick, we mutated 2 sites (EcoRI and PstI) inside the FliA sequence by assemby PCR. <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" |[[Image:Icon_coding.png]]<br>FlhDC<br />
|align=| FlhDC is the main regulator of the flagella genetic system in E. coli. FlhDC regulates all the promoter of the FIFO.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}<br />
<br />
{{Paris/Navig|Team:Paris/Construction}}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T04:06:00Z
<p>Landrain: /* Synchronization module */</p>
<hr />
<div>{{Paris/Menu}}<br />
=Model constructions: from the modelling to the characterization=<br />
<br />
Our project BacterioClock is based on an oscillating FIFO synchronized at the population level. To obtain and have preliminary results of this system we divided it in three "little" modules as the modeling team exposed them previously.<br />
==The First is to demonstrate the [[Team:Paris/Analysis#FIFO_behaviour|FIFO]] which constitutes our core system==<br />
<br />
A simple manner is to implement two class II promotor followed by fluorescent reporter genes like GFP in a bacteria strain that is able to produce the flagella. <br />
<br />
By this way, we are able to see in single cell the order of the activation of pFliL then pFlgA then pFlhB and then the inactivation in the same order as a FIFO will do.<br />
<br />
These constructions are equivalent to the experiments realized by Uri Alon in the article "Using quantitative Blueprint to reprogram the Dynamics of the Flagella Gene Network" Kalir S, Alon U. Cell 2004.<br />
<br />
A better way to observe the FIFO is to study the system with an inducible regulator of class II gene in a bacteria strain deleted for this gene. We are extremely grateful for Alon U. that send us the inducible gene ''FlhDC'', and ''FliA'' in pBad18 plasmid.<br />
These plasmids with our own araC/pBad-EnvZ* will allowed us to study the system in mutated strains that we have in our lab (ΔFlhD, ΔFliA, ΔFlgM, ΔEnvZ). By cotransforming in mutated strains the inducibles regulators of the class II promotors with one of this promotor associated to a fluorescent protein, we could characterize the influence of the master regulators of the flagella on their pormoter. The fluorescence is normalized to the OD<sub>600</sub>.<br />
<br>For example below are some constructions to see and induce the FIFO:<br />
<br><br><br><br />
[[Image:Felipe01.jpg|500px|center|thumb|pFliL alone]]<br><br><br><br />
[[Image:Felipe02.jpg|500px|center|thumb|3 promoters of the FIFO in one Cell]]<br><br><br><br />
[[Image:Felipe03.jpg|500px|center|thumb|FIFO and oscillating construction in a single cell]]<br><br><br><br />
<br />
<br />
To perform such a cotransformation we take care about the ORI of each low copy plasmid which are often incompatible and we made all our final constructions in the pSB4T5 plasmid that care the only one resistance that was not already used and the pSC101 ORI which is compatible with the ColE1.<br />
<br />
We could see the FIFO in a more convenient manner with the assembly of two class II promotors associated to differents fluorophores. Then we could observed two kinds of fluorescents in One Cell along time. <br />
One problem that could occur is the interaction between the differents fluorescents proteins due to the cross-over of their emission and excitation spectra and moreover in our final system that count three fluorophores.<br />
<br />
[[Image:Spectre.jpg |600px |thumb| emission and excitation spectra of ECFP, YFP and mRFP]]<br />
<br />
<br>The choice of ECFP, YFP and mRFP is based upon this consideration and upon the order of appearing in the FIFO.<br />
Indeed by putting the mRFP in last position, we improve the visualization of the FIFO like the maturation time of the protein is about of one hour.<br />
<br />
We thank a lot Arnau Montagu, advisor of the iGEM Valencia Team. He advised us, after his team had troubleshooting with fluorescence, to take care about ''spurious FRET''. This phenomenon occurs even with non covered fluorescent spectra when the concentration of two fluorophores is sufficient so they are enough closer to induce some FRET. But it could happen at lower concentration than 40 μM. <br><br />
<br>- "DsRed as a Potential FRET Partner with CFP and GFP" Michael G. Erickson, Daniel L. Moon, and David T. Yue Biophysical Journal 2003. <br />
<br>- "A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP<br />
Sensor as an Example" Gerard N. M. van der Krogt, Janneke Ogink, Bas Ponsioen, Kees Jalink PLoS 2008.<br />
<br />
<br><br />
<br />
==[[Team:Paris/Analysis#Oscillations|Oscillating]] module==<br />
Few approaches have been thought in order to create the oscillating module. The pTet/TetR system that allows us to precisely control the inhibition of TetR on the Tet promotor. Another system use the natural inhibition of OmpR and of EnvZ via OmpR. We finally perform this system that we have developed since we know thanks to the modeling team that the oscillations are the only way to see the full development of the FIFO.<br />
<br />
==Synchronization module==<br />
The modeling simulation showed us that to have some oscillations that are difficult to obtain in a simple feedforward loop without the delay introduce by the quorum sensing synchronization, and for us it is more interesting to have the FIFO behavior at the population level. We did not achieved the this module but we started the construction of it and have already some intermediate parts that you will find below. <br />
The constructions we were able to obtain allow us to see the pictures below of the fluorescence of one of the promoter of the FIFO Flagella:<br />
<br />
== Table of the constructions we realized==<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136039 pFliL(RBS WT)-ECFP tripart (LVA+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136037 pFliL(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136012 pFlgA(+ RBS WT) - YFP Tripart (LVA+)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136013 pFlgA(+ RBS WT) - YFP Tripart (LVA-)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136054 pFlgA(+ RBS WT)-YFP tripart (AAV+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136053 pFlgA(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136055 pFlhB(+ RBS WT)-mRFP tripart (+LVA)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136065 pFlhB(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136064 pFliL(+ RBS WT)-EnvZ*]<br />
|align=| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136069 pFliL(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136051 pFlhB(+ RBS WT)-EnvZ*]<br />
|align=| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136070 pFlhB(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136066 araC/pBad-EnvZ*]<br />
|align=| A construction made for characterizing the repression power of the mutated EnvZ on our system<br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136029 pLas - gfp Tripart]<br />
|align=| Construction for characterization of the pLas promoter<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136027 pLas - ECFP]<br />
|align=| The same as before but with another fluorophore<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136024 strongest promoter-rbs-lasR-dble ter]<br />
|align=| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136023 Strong promoter-rbs-lasR-dble ter]<br />
|align=| A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria.<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136044 Strongest RBS (1)- LasR activator (+LVA)]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136048 rbs-lasR + dbl ter]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136052 lasR activator + LVA - Double Terminator]<br />
|align=| Intermediary construction<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136057 RBS lasI - GFP tripart]<br />
|align=| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136056 RBS-lasI - ECFP]<br />
|align=| The same as the previous one but with another fluorophore.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136010 pFliA]<br />
|align=| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.<br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136009 pFliL (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136006 pFlgA (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA..<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br> pFlgB (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136008 pFlhB (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136046 EnvZ*]<br />
|align=| EnvZ is a membrane protein involved into the osmotic response and is interacting with OmpR that will bind the promoter of FlhDC ad will repress it. EnvZ* is a mutated form of the coding sequence, giving a phenotype where pFlhDC is completely repressed. EnvZ has been extracted by PVR for the correct mutant strain.<br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align=| FliA is a sigma factor involved into the regulation of very various genes, the promoter used in the FIFO were selected to be higly responding to FliA. In order to have the FliA gene possibly used as biobrick, we mutated 2 sites (EcoRI and PstI) inside the FliA sequence by assemby PCR. <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" |[[Image:Icon_coding.png]]<br>FlhDC<br />
|align=| FlhDC is the main regulator of the flagella genetic system in E. coli. FlhDC regulates all the promoter of the FIFO.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}<br />
<br />
{{Paris/Navig|Team:Paris/Construction}}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T04:01:35Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
=Model constructions: from the modelling to the characterization=<br />
<br />
Our project BacterioClock is based on an oscillating FIFO synchronized at the population level. To obtain and have preliminary results of this system we divided it in three "little" modules as the modeling team exposed them previously.<br />
==The First is to demonstrate the [[Team:Paris/Analysis#FIFO_behaviour|FIFO]] which constitutes our core system==<br />
<br />
A simple manner is to implement two class II promotor followed by fluorescent reporter genes like GFP in a bacteria strain that is able to produce the flagella. <br />
<br />
By this way, we are able to see in single cell the order of the activation of pFliL then pFlgA then pFlhB and then the inactivation in the same order as a FIFO will do.<br />
<br />
These constructions are equivalent to the experiments realized by Uri Alon in the article "Using quantitative Blueprint to reprogram the Dynamics of the Flagella Gene Network" Kalir S, Alon U. Cell 2004.<br />
<br />
A better way to observe the FIFO is to study the system with an inducible regulator of class II gene in a bacteria strain deleted for this gene. We are extremely grateful for Alon U. that send us the inducible gene ''FlhDC'', and ''FliA'' in pBad18 plasmid.<br />
These plasmids with our own araC/pBad-EnvZ* will allowed us to study the system in mutated strains that we have in our lab (ΔFlhD, ΔFliA, ΔFlgM, ΔEnvZ). By cotransforming in mutated strains the inducibles regulators of the class II promotors with one of this promotor associated to a fluorescent protein, we could characterize the influence of the master regulators of the flagella on their pormoter. The fluorescence is normalized to the OD<sub>600</sub>.<br />
<br>For example below are some constructions to see and induce the FIFO:<br />
<br><br><br><br />
[[Image:Felipe01.jpg|500px|center|thumb|pFliL alone]]<br><br><br><br />
[[Image:Felipe02.jpg|500px|center|thumb|3 promoters of the FIFO in one Cell]]<br><br><br><br />
[[Image:Felipe03.jpg|500px|center|thumb|FIFO and oscillating construction in a single cell]]<br><br><br><br />
<br />
<br />
To perform such a cotransformation we take care about the ORI of each low copy plasmid which are often incompatible and we made all our final constructions in the pSB4T5 plasmid that care the only one resistance that was not already used and the pSC101 ORI which is compatible with the ColE1.<br />
<br />
We could see the FIFO in a more convenient manner with the assembly of two class II promotors associated to differents fluorophores. Then we could observed two kinds of fluorescents in One Cell along time. <br />
One problem that could occur is the interaction between the differents fluorescents proteins due to the cross-over of their emission and excitation spectra and moreover in our final system that count three fluorophores.<br />
<br />
[[Image:Spectre.jpg |600px |thumb| emission and excitation spectra of ECFP, YFP and mRFP]]<br />
<br />
<br>The choice of ECFP, YFP and mRFP is based upon this consideration and upon the order of appearing in the FIFO.<br />
Indeed by putting the mRFP in last position, we improve the visualization of the FIFO like the maturation time of the protein is about of one hour.<br />
<br />
We thank a lot Arnau Montagu, advisor of the iGEM Valencia Team. He advised us, after his team had troubleshooting with fluorescence, to take care about ''spurious FRET''. This phenomenon occurs even with non covered fluorescent spectra when the concentration of two fluorophores is sufficient so they are enough closer to induce some FRET. But it could happen at lower concentration than 40 μM. <br><br />
<br>- "DsRed as a Potential FRET Partner with CFP and GFP" Michael G. Erickson, Daniel L. Moon, and David T. Yue Biophysical Journal 2003. <br />
<br>- "A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP<br />
Sensor as an Example" Gerard N. M. van der Krogt, Janneke Ogink, Bas Ponsioen, Kees Jalink PLoS 2008.<br />
<br />
<br><br />
<br />
==[[Team:Paris/Analysis#Oscillations|Oscillating]] module==<br />
Few approaches have been thought in order to create the oscillating module. The pTet/TetR system that allows us to precisely control the inhibition of TetR on the Tet promotor. Another system use the natural inhibition of OmpR and of EnvZ via OmpR. We finally perform this system that we have developed since we know thanks to the modeling team that the oscillations are the only way to see the full development of the FIFO.<br />
<br />
==Synchronization module==<br />
The modeling simulation showed us that to have some oscillation that are difficult to obtain in a simple feedforward loop without the delay introduce by the quorum sensing synchronization, and for us it is more interesting to have the FIFO behavior at the population level. We did not achieved the this module but we started the construction of it and have already some intermediate parts that you will find below.<br />
<br />
== Table of the constructions we realized==<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136039 pFliL(RBS WT)-ECFP tripart (LVA+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136037 pFliL(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136012 pFlgA(+ RBS WT) - YFP Tripart (LVA+)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136013 pFlgA(+ RBS WT) - YFP Tripart (LVA-)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136054 pFlgA(+ RBS WT)-YFP tripart (AAV+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136053 pFlgA(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136055 pFlhB(+ RBS WT)-mRFP tripart (+LVA)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136065 pFlhB(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136064 pFliL(+ RBS WT)-EnvZ*]<br />
|align=| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136069 pFliL(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136051 pFlhB(+ RBS WT)-EnvZ*]<br />
|align=| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136070 pFlhB(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136066 araC/pBad-EnvZ*]<br />
|align=| A construction made for characterizing the repression power of the mutated EnvZ on our system<br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136029 pLas - gfp Tripart]<br />
|align=| Construction for characterization of the pLas promoter<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136027 pLas - ECFP]<br />
|align=| The same as before but with another fluorophore<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136024 strongest promoter-rbs-lasR-dble ter]<br />
|align=| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136023 Strong promoter-rbs-lasR-dble ter]<br />
|align=| A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria.<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136044 Strongest RBS (1)- LasR activator (+LVA)]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136048 rbs-lasR + dbl ter]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136052 lasR activator + LVA - Double Terminator]<br />
|align=| Intermediary construction<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136057 RBS lasI - GFP tripart]<br />
|align=| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136056 RBS-lasI - ECFP]<br />
|align=| The same as the previous one but with another fluorophore.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136010 pFliA]<br />
|align=| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.<br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136009 pFliL (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136006 pFlgA (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA..<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br> pFlgB (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136008 pFlhB (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136046 EnvZ*]<br />
|align=| EnvZ is a membrane protein involved into the osmotic response and is interacting with OmpR that will bind the promoter of FlhDC ad will repress it. EnvZ* is a mutated form of the coding sequence, giving a phenotype where pFlhDC is completely repressed. EnvZ has been extracted by PVR for the correct mutant strain.<br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align=| FliA is a sigma factor involved into the regulation of very various genes, the promoter used in the FIFO were selected to be higly responding to FliA. In order to have the FliA gene possibly used as biobrick, we mutated 2 sites (EcoRI and PstI) inside the FliA sequence by assemby PCR. <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" |[[Image:Icon_coding.png]]<br>FlhDC<br />
|align=| FlhDC is the main regulator of the flagella genetic system in E. coli. FlhDC regulates all the promoter of the FIFO.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}<br />
<br />
{{Paris/Navig|Team:Paris/Construction}}</div>
Landrain
http://2008.igem.org/Team:Paris
Team:Paris
2008-10-30T03:54:16Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
<html><div style="text-align:justify; background: url(https://static.igem.org/mediawiki/2008/f/fc/IGEM_PARIS_2008_fond4.jpg); background-repeat:no-repeat; margin=0;"></html><br />
<br><br />
<center><html><div style="color:#275D96; font-size:4em;">The BacteriO'Clock</div></html></center><br />
<br><br />
<br><br />
<br />
<center><br />
<html><br />
<embed width="320" height="240" src="http://biosynthetique.free.fr/videos/flv_player/flvplayer.swf?autostart=true&amp;repeat&amp;file=http://perso.strepsiade.org/~gvieira/out2.flv" quality="high" type="application/x-shockwave-flash" allowfullscreen="true" /><br />
</html><br />
</center><br />
<br />
<br><br />
<br><br />
Have you ever dreamed of a new way of thinking everyday life objects? Be sure, this will come true, under the form of our '''BacteriO'Clock !''' A simple test tube which contains our modified bacteria will give you the time, directly from living organisms. The hours of the day are '''color-coded''' and '''oscillations''' ensure the repeated periodic behavior. Inspired by the flagellar motor machine, our clock serves as a chassis to be used in building the future molecular modular machines in an optimized and coordinated manner. It may serve as well to control in an optimized fashion '''multi-reaction pathways'''. <br />
<br />
<br><br />
To achieve this incredible project, we relied on a well characterized genetic structure that allows a specific sequence of fluorescent proteins expression to occur in a First In - First Out order. This '''FIFO''' behavior enables us to define a rich color encoding of day time. An additional negative feedback loop generates clock oscillations! <br />
<br><br><br />
Based on in-depth studies and experimentally measured parameters we developed predictive models that suggest that this core system is not likely to demonstrate stable oscillations due to damping phenomenon leading to a stationary steady-state. Importantly, our modeling approach provided us with alternative improved designs that should lead in principle to an optimized '''BacteriO'Clock'''. In particular, this is achieved through '''synchronization at the population level''' by adopting a quorum sensing feedback mediated by HSL production that enforces a necessary delay and provides an elegant cellular synchronization mechanism : <br />
<br><br><br><br />
<br />
[[Image:Unimo.png|400px|thumb|right|Simple visualization of our color coded BacteriO'Clock]]<br />
<br />
<br />
<br><br />
<br><br />
<br><br />
<br><br />
In a nutshell, the new trendy item which will make every biologist in your lab jealous will soon be available. Through a long-cloning period, we now have all biobricks needed and much of the final constructions. Furthermore, we initiated an extensive characterization plan of these parts. If you manage to wait for the updates, you might even get the version that automatically reacts to daylight saving time ;)<br />
<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
=We are extremely grateful to the organizations that support our project=<br />
{|<br />
|width=25%| [[Image:bettencourt.png]]<br />
<br />
Bettencourt-Schueller Foundation<br />
<br />
[http://www.fondationbs.org/ www.fondationbs.org]<br />
<br />
|width=25%| [[Image:Logocri.jpg|115px]]<br />
<br />
Centre de Recherches Interdisciplinaires<br />
<br />
[http://www.cri-paris.org/fr/cri/ www.cri-paris.org/fr/cri/]<br />
<br />
|width=25%| [[Image:LogoPCU1.jpg|100px]]<br />
<br />
Paris Centre universités<br />
<br />
[http://www.pariscentreuniversites.fr/ www.pariscentreuniversites.fr]<br />
<br />
|-<br />
|-<br />
|-<br />
<br />
|[[Image:Embassy.png|250px]]<br />
<br />
Embassy of France in the United States<br />
<br />
[http://www.ambafrance-us.org/ www.ambafrance-us.org]<br />
<br />
|[[Image:FacMed.png|140px]]<br />
Faculté de Médecine Université Paris Descartes<br />
<br />
[http://www.medecine.univ-paris5.fr/ www.medecine.univ-paris5.fr]<br />
<br />
|[[Image:Qiagen.GIF|60px]]<br />
<br />
Qiagen<br />
<br />
[http://www.qiagen.com/ www.qiagen.com/]<br />
|-<br />
|-<br />
|-<br />
<br />
|[[Image:Ibisc.jpeg|100px]]<br />
<br />
Ibisc Laboratory<br />
<br />
[http://www.ibisc.univ-evry.fr/ www.ibisc.univ-evry.fr]<br />
<br />
<br />
|[[Image:Gdrbim.jpg|100px]]<br />
<br />
GdR BiM<br />
<br />
[http://www.gdr-bim.u-psud.fr/ www.gdr-bim.u-psud.fr]<br />
<br />
|[[Image:Logo INRIA.jpg|180px]]<br />
<br />
INRIA<br />
<br />
[http://www.inria.fr/ www.inria.fr]<br />
<br />
|}<br />
<hr /><br />
<center><html><br />
<a href="http://www4.clustrmaps.com/counter/maps.php?url=https://2008.igem.org/Team:Paris" id="clustrMapsLink"><br />
<img src="http://www4.clustrmaps.com/counter/index2.php?url=https://2008.igem.org/Team:Paris" border="0" onerror="this.onerror=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com';" /><br />
</a><br />
<br /><br />
<a href="http://www.easycounter.com/"><br />
<img src="http://www.easycounter.com/counter.php?bloup" border="0" /><br />
</a><br />
</html></center></div>
Landrain
http://2008.igem.org/Team:Paris
Team:Paris
2008-10-30T03:52:22Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
<html><div style="text-align:justify; background: url(https://static.igem.org/mediawiki/2008/f/fc/IGEM_PARIS_2008_fond4.jpg); background-repeat:no-repeat; margin=0;"></html><br />
<br><br />
<center><html><div style="color:#275D96; font-size:4em;">The BacteriO'Clock</div></html></center><br />
<br><br />
<br><br />
<br />
<center><br />
<html><br />
<embed width="320" height="240" src="http://biosynthetique.free.fr/videos/flv_player/flvplayer.swf?autostart=true&amp;repeat&amp;file=http://perso.strepsiade.org/~gvieira/out2.flv" quality="high" type="application/x-shockwave-flash" allowfullscreen="true" /><br />
</html><br />
</center><br />
<br />
<br><br />
<br><br />
Have you ever dreamed of a new way of thinking everyday life objects? Be sure, this will come true, under the form of our '''BacteriO'Clock !''' A simple test tube which contains our modified bacteria will give you the time, directly from living organisms. The hours of the day are color-coded and oscillations ensure the repeated periodic behavior. Inspired by the flagellar motor machine, our clock serves as a chassis to be used in building the future molecular modular machines in an optimized and coordinated manner. It may serve as well to control in an optimized fashion multi-reaction pathways. <br />
<br />
<br><br />
To achieve this incredible project, we relied on a well characterized genetic structure that allows a specific sequence of fluorescent proteins expression to occur in a First In - First Out order. This FIFO behavior enables us to define a rich color encoding of day time. An additional negative feedback loop generates clock oscillations! <br />
<br><br><br />
Based on in-depth studies and experimentally measured parameters we developed predictive models that suggest that this core system is not likely to demonstrate stable oscillations due to damping phenomenon leading to a stationary steady-state. Importantly, our modeling approach provided us with alternative improved designs that should lead in principle to an optimized BacteriO'Clock. In particular, this is achieved through synchronization at the population level by adopting a quorum sensing feedback mediated by HSL production that enforces a necessary delay and provides an elegant cellular synchronization mechanism : <br />
<br><br><br><br />
<br />
[[Image:Unimo.png|400px|thumb|right|Simple visualization of our color coded BacteriO'Clock]]<br />
<br />
<br />
<br><br />
<br><br />
<br><br />
<br><br />
In a nutshell, the new trendy item which will make every biologist in your lab jealous will soon be available. Through a long-cloning period, we now have all biobricks needed and much of the final constructions. Furthermore, we initiated an extensive characterization plan of these parts. If you manage to wait for the updates, you might even get the version that automatically reacts to daylight saving time ;)<br />
<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
=We are extremely grateful to the organizations that support our project=<br />
{|<br />
|width=25%| [[Image:bettencourt.png]]<br />
<br />
Bettencourt-Schueller Foundation<br />
<br />
[http://www.fondationbs.org/ www.fondationbs.org]<br />
<br />
|width=25%| [[Image:Logocri.jpg|115px]]<br />
<br />
Centre de Recherches Interdisciplinaires<br />
<br />
[http://www.cri-paris.org/fr/cri/ www.cri-paris.org/fr/cri/]<br />
<br />
|width=25%| [[Image:LogoPCU1.jpg|100px]]<br />
<br />
Paris Centre universités<br />
<br />
[http://www.pariscentreuniversites.fr/ www.pariscentreuniversites.fr]<br />
<br />
|-<br />
|-<br />
|-<br />
<br />
|[[Image:Embassy.png|250px]]<br />
<br />
Embassy of France in the United States<br />
<br />
[http://www.ambafrance-us.org/ www.ambafrance-us.org]<br />
<br />
|[[Image:FacMed.png|150px]]<br />
Faculté de Médecine Université Paris Descartes<br />
<br />
[http://www.medecine.univ-paris5.fr/ www.medecine.univ-paris5.fr]<br />
<br />
|[[Image:Qiagen.GIF|70px]]<br />
<br />
Qiagen<br />
<br />
[http://www.qiagen.com/ www.qiagen.com/]<br />
|-<br />
|-<br />
|-<br />
<br />
|[[Image:Ibisc.jpeg|100px]]<br />
<br />
Ibisc Laboratory<br />
<br />
[http://www.ibisc.univ-evry.fr/ www.ibisc.univ-evry.fr]<br />
<br />
<br />
|[[Image:Gdrbim.jpg|100px]]<br />
<br />
GdR BiM<br />
<br />
[http://www.gdr-bim.u-psud.fr/ www.gdr-bim.u-psud.fr]<br />
<br />
|[[Image:Logo INRIA.jpg|180px]]<br />
<br />
INRIA<br />
<br />
[http://www.inria.fr/ www.inria.fr]<br />
<br />
|}<br />
<hr /><br />
<center><html><br />
<a href="http://www4.clustrmaps.com/counter/maps.php?url=https://2008.igem.org/Team:Paris" id="clustrMapsLink"><br />
<img src="http://www4.clustrmaps.com/counter/index2.php?url=https://2008.igem.org/Team:Paris" border="0" onerror="this.onerror=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com';" /><br />
</a><br />
<br /><br />
<a href="http://www.easycounter.com/"><br />
<img src="http://www.easycounter.com/counter.php?bloup" border="0" /><br />
</a><br />
</html></center></div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T03:43:23Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136039 pFliL(RBS WT)-ECFP tripart (LVA+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136037 pFliL(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136012 pFlgA(+ RBS WT) - YFP Tripart (LVA+)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136013 pFlgA(+ RBS WT) - YFP Tripart (LVA-)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136054 pFlgA(+ RBS WT)-YFP tripart (AAV+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136053 pFlgA(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136055 pFlhB(+ RBS WT)-mRFP tripart (+LVA)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136065 pFlhB(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136064 pFliL(+ RBS WT)-EnvZ*]<br />
|align=| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136069 pFliL(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136051 pFlhB(+ RBS WT)-EnvZ*]<br />
|align=| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136070 pFlhB(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136066 araC/pBad-EnvZ*]<br />
|align=| A construction made for characterizing the repression power of the mutated EnvZ on our system<br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136029 pLas - gfp Tripart]<br />
|align=| Construction for characterization of the pLas promoter<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136027 pLas - ECFP]<br />
|align=| The same as before but with another fluorophore<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136024 strongest promoter-rbs-lasR-dble ter]<br />
|align=| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136023 Strong promoter-rbs-lasR-dble ter]<br />
|align=| A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria.<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136044 Strongest RBS (1)- LasR activator (+LVA)]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136048 rbs-lasR + dbl ter]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136052 lasR activator + LVA - Double Terminator]<br />
|align=| Intermediary construction<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136057 RBS lasI - GFP tripart]<br />
|align=| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136056 RBS-lasI - ECFP]<br />
|align=| The same as the previous one but with another fluorophore.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136010 pFliA]<br />
|align=| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.<br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136009 pFliL (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136006 pFlgA (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA..<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br> pFlgB (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136008 pFlhB (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136046 EnvZ*]<br />
|align=| EnvZ is a membrane protein involved into the osmotic response and is interacting with OmpR that will bind the promoter of FlhDC ad will repress it. EnvZ* is a mutated form of the coding sequence, giving a phenotype where pFlhDC is completely repressed. EnvZ has been extracted by PVR for the correct mutant strain.<br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align=| FliA is a sigma factor involved into the regulation of very various genes, the promoter used in the FIFO were selected to be higly responding to FliA. In order to have the FliA gene possibly used as biobrick, we mutated 2 sites (EcoRI and PstI) inside the FliA sequence by assemby PCR. <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" |[[Image:Icon_coding.png]]<br>FlhDC<br />
|align=| FlhDC is the main regulator of the flagella genetic system in E. coli. FlhDC regulates all the promoter of the FIFO.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T03:42:37Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136039 pFliL(RBS WT)-ECFP tripart (LVA+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136037 pFliL(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136012 pFlgA(+ RBS WT) - YFP Tripart (LVA+)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136013 pFlgA(+ RBS WT) - YFP Tripart (LVA-)]<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136054 pFlgA(+ RBS WT)-YFP tripart (AAV+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136053 pFlgA(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136055 pFlhB(+ RBS WT)-mRFP tripart (+LVA)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>[http://partsregistry.org/Part:BBa_K136065 pFlhB(+ RBS WT)-GFP]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136064 pFliL(+ RBS WT)-EnvZ*]<br />
|align=| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136069 pFliL(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136051 pFlhB(+ RBS WT)-EnvZ*]<br />
|align=| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136070 pFlhB(+ RBS WT)-EnvZ*-GFP generator]<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136066 araC/pBad-EnvZ*]<br />
|align=| A construction made for characterizing the repression power of the mutated EnvZ on our system<br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136029 pLas - gfp Tripart]<br />
|align=| Construction for characterization of the pLas promoter<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136027 pLas - ECFP]<br />
|align=| The same as before but with another fluorophore<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136024 strongest promoter-rbs-lasR-dble ter]<br />
|align=| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136023 Strong promoter-rbs-lasR-dble ter]<br />
|align=| A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria.<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136044 Strongest RBS (1)- LasR activator (+LVA)]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136048 rbs-lasR + dbl ter]<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136052 lasR activator + LVA - Double Terminator]<br />
|align=| Intermediary construction<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136057 RBS lasI - GFP tripart]<br />
|align=| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> [http://partsregistry.org/Part:BBa_K136056 RBS-lasI - ECFP]<br />
|align=| The same as the previous one but with another fluorophore.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136010 pFliA]<br />
|align=| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.<br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136009 pFliL (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136006 pFlgA (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA..<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br> pFlgB (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>[http://partsregistry.org/Part:BBa_K136008 pFlhB (+RBS WT)]<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>[http://partsregistry.org/Part:BBa_K136046 EnvZ*]<br />
|align=| EnvZ is a membrane protein involved into the osmotic response and is interacting with OmpR that will bind the promoter of FlhDC ad will repress it. EnvZ* is a mutated form of the coding sequence, giving a phenotype where pFlhDC is completely repressed. EnvZ has been extracted by PVR for the correct mutant strain.<br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | FliA<br />
|align=| FliA is a sigma factor involved into the regulation of very various genes, the promoter used in the FIFO were selected to be higly responding to FliA. In order to have the FliA gene possibly used as biobrick, we mutated 2 sites (EcoRI and PstI) inside the FliA sequence by assemby PCR. <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | FlhDC<br />
|align=| FlhDC is the main regulator of the flagella genetic system in E. coli. FlhDC regulates all the promoter of the FIFO.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T03:27:52Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>[http://partsregistry.org/Part:BBa_K136039 pFliL(RBS WT)-ECFP tripart (LVA+)]<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA+)<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA-)<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align=| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ RBS WT)-EnvZ*-GFP generator<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align=| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align=| A construction made for characterizing the repression power of the mutated EnvZ on our system<br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align=| Construction for characterization of the pLas promoter<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align=| The same as before but with another fluorophore<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align=| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align=| A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria.<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align=| Intermediary construction<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align=| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align=| The same as the previous one but with another fluorophore.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align=| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.<br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA..<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align=| EnvZ is a membrane protein involved into the osmotic response and is interacting with OmpR that will bind the promoter of FlhDC ad will repress it. EnvZ* is a mutated form of the coding sequence, giving a phenotype where pFlhDC is completely repressed. EnvZ has been extracted by PVR for the correct mutant strain.<br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align=| FliA is a sigma factor involved into the regulation of very various genes, the promoter used in the FIFO were selected to be higly responding to FliA. In order to have the FliA gene possibly used as biobrick, we mutated 2 sites (EcoRI and PstI) inside the FliA sequence by assemby PCR. <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align=| FlhDC is the main regulator of the flagella genetic system in E. coli. FlhDC regulates all the promoter of the FIFO.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T03:20:30Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA+)<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA-)<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align=| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ RBS WT)-EnvZ*-GFP generator<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align=| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align=| A construction made for characterizing the repression power of the mutated EnvZ on our system<br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align=| Construction for characterization of the pLas promoter<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align=| The same as before but with another fluorophore<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align=| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align=| A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria.<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align=| Intermediary construction<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align=| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align=| The same as the previous one but with another fluorophore.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align=| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.<br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA..<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.<br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align=| EnvZ is a membrane protein involved into the osmotic response and is interacting with OmpR that will bind the promoter of FlhDC ad will repress it. EnvZ* is a mutated form of the coding sequence, giving a phenotype where pFlhDC is completely repressed. EnvZ has been extracted by PVR for the correct mutant strain.<br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align=| FliA is a sigma factor involved into the regulation of very various genes, the promoter used in the FIFO were selected to be higly responding to FliA. In order to have the FliA gene possibly used as biobrick, we mutated 2 sites (EcoRI and PstI) inside the FliA sequence by assemby PCR. <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align=| FlhDC is the main regulator of the flagella genetic system in E. coli. FlhDC regulates all the promoter of the FIFO.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T03:05:14Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA+)<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA-)<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align=| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ RBS WT)-EnvZ*-GFP generator<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align=| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.<br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align=| A construction made for characterizing the repression power of the mutated EnvZ on our system<br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align=| Construction for characterization of the pLas promoter<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align=| The same as before but with another fluorophore<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align=| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align=| A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria.<br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL<br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align=| Intermediary construction<br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align=| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align=| The same as the previous one but with another fluorophore.<br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align=| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.<br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align=| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align=| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align=| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align=| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align=| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align=| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align=| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T02:49:41Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA+)<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA-)<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align=| <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ RBS WT)-EnvZ*-GFP generator<br />
|align=| <br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align=| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align=| <br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align=| <br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align=| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align=| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align=| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align=| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align=| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align=| <br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align=| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align=| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align=| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align=| <br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align=| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align=| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align=| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align=| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align=| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align=| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align=| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T02:47:35Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA+)<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA-)<br />
|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align="center"| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T02:40:26Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFLiL is ECFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T02:39:38Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFLiL is ECFP.<br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T02:34:51Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 29|October 29]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 27|October 27]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T02:33:31Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 22|August 22]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 24|October 24]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 26|October 26]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T02:26:07Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 20|August 20]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 19|August 19]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 1|August 1]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T02:13:48Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| <br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 04|October 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align="center"| <br />
|align="center"| [[Team:Paris/October 10|October 10]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align="center"| <br />
|align="center"| [[Team:Paris/September 04|September 04]]<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align="center"| <br />
|align="center"| [[Team:Paris/August 13|August 13]]<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T02:11:11Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=55%| Description<br />
|width=15%| Validation Date<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| <br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align="center"| <br />
|align="center"| <br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align="center"| <br />
|align="center"| <br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T02:03:17Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T02:02:17Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+ WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T02:00:31Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''New Gene And Promoter Biobricks'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:59:28Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''New Gene And Promoter Biobricks'''</center><br />
|-<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:58:26Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''New Gene And Promoter Biobricks'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:47:10Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP <br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:26:41Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:26:02Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:24:50Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:23:13Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:22:06Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:19:59Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(RBS WT)-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+WT RBS)-EnvZ*-GFP generator<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:11:56Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:11:27Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
!colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''Ftecty'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:11:08Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;" colspan=4 style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''Ftecty'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:10:47Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | <center>'''Ftecty'''</center><br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:10:22Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="text-align=center;"<br />
|colspan=4 style="background: #649cd7" | '''Ftecty'''<br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:09:25Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" text-align=center | '''Ftecty'''<br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:09:00Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7; text-align=center;" | Ftecty<br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:07:55Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4 style="background: #649cd7" | Ftecty<br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:07:35Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4|style="background: #649cd7" | Ftecty<br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:06:50Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|-<br />
|colspan=4|<br />
|-<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:05:46Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T01:03:42Z
<p>Landrain: /* PCR products */</p>
<hr />
<div>{{Paris/Menu}}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}<br />
<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T00:59:58Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
== PCR products ==<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(RBS WT)-mRFP tripart (+LVA)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T00:57:29Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
== PCR products ==<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=30%| Construction<br />
|width=50%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T00:57:12Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
== PCR products ==<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=35%| Construction<br />
|width=45%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T00:55:28Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
== PCR products ==<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=35%| Construction<br />
|width=45%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"||<br />
- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain
http://2008.igem.org/Team:Paris/Construction
Team:Paris/Construction
2008-10-30T00:54:22Z
<p>Landrain: </p>
<hr />
<div>{{Paris/Menu}}<br />
== PCR products ==<br />
{|<br />
|- style="background: #649cd7;"<br />
! colspan="10" style="background: #649cd7" | FIFO<br />
|- style="background: #649cd7; text-align: center;"<br />
|width=35%| Construction<br />
|width=45%| Description<br />
|width=10%| Validation Date<br />
|width=10%| Link to Notebook<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | <br />
[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(RBS WT)-YFP tripart (AAV+)<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"||- style="background: #dddddd;" <br />
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(RBS WT)-GFP<br />
|align="center"| <br />
|align="center"| <br />
|align="center"|<br />
|}</div>
Landrain