http://2008.igem.org/wiki/index.php?title=Special:Contributions/Trentmortensen&feed=atom&limit=50&target=Trentmortensen&year=&month=2008.igem.org - User contributions [en]2024-03-28T18:37:11ZFrom 2008.igem.orgMediaWiki 1.16.5http://2008.igem.org/Utah_State/28_October_2008Utah State/28 October 20082008-10-29T20:19:42Z<p>Trentmortensen: New page: Cultures were inoculated in preparation for isolation of BioBricks tomorrow.</p>
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<div>Cultures were inoculated in preparation for isolation of BioBricks tomorrow.</div>Trentmortensenhttp://2008.igem.org/Utah_State/22_October_2008Utah State/22 October 20082008-10-24T17:53:12Z<p>Trentmortensen: </p>
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<div>Transformations from yesterday produced bacterial lawns. New ampicillin solution was made and used in new plates. <br />
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PCR was performed for 5'F/SD R and -35F/ATG R and digested with E/S. This required a sequential digestion of Spe1 first then EcoR1 + additional 10X buffer O according to manufacturer's instructions.</div>Trentmortensenhttp://2008.igem.org/Utah_State/21_October_2008Utah State/21 October 20082008-10-24T17:52:07Z<p>Trentmortensen: New page: Transformations were performed with yesterday's ligations.</p>
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<div>Transformations were performed with yesterday's ligations.</div>Trentmortensenhttp://2008.igem.org/Utah_State/20_October_2008Utah State/20 October 20082008-10-24T17:51:35Z<p>Trentmortensen: New page: Six ligations were performed, three with new T4 ligase and three with older T4 ligase: -35 Pro in pSB1A3, 5'F/SD R in pSB1A3, and phaB in pSB1A3. Cryostocks were made of 5'F/ATG R, 2x ...</p>
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<div>Six ligations were performed, three with new T4 ligase and three with older T4 ligase: -35 Pro in pSB1A3, 5'F/SD R in pSB1A3, and phaB in pSB1A3. <br />
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Cryostocks were made of 5'F/ATG R, 2x 5'F/TC R, and -35F/TC R</div>Trentmortensenhttp://2008.igem.org/Utah_State/22_October_2008Utah State/22 October 20082008-10-24T17:48:43Z<p>Trentmortensen: New page: PCR was performed for 5'F/SD R and -35F/ATG R and digested with E/S. This required a sequential digestion of Spe1 first then EcoR1 + additional 10X buffer O according to manufacturer's in...</p>
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<div>PCR was performed for 5'F/SD R and -35F/ATG R and digested with E/S. This required a sequential digestion of Spe1 first then EcoR1 + additional 10X buffer O according to manufacturer's instructions.</div>Trentmortensenhttp://2008.igem.org/Utah_State/23_October_2008Utah State/23 October 20082008-10-24T17:46:30Z<p>Trentmortensen: </p>
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<div>Yesterday's PCR products were run out on a gel (5'F/SD R, -35F/ATG R), which showed that the PCR was unsuccessful. <br />
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PCR was performed for two PHB promoter regions and two pha gene regions, respectively: 5'F/SD R and -35F/ATG R, phaB and phaCAB.</div>Trentmortensenhttp://2008.igem.org/Utah_State/23_October_2008Utah State/23 October 20082008-10-24T17:45:21Z<p>Trentmortensen: New page: Yesterday's PCR products were run out on a gel (5'F/SD R, -35F/ATG R), which showed that the PCR was unsuccessful. PCR was performed for two PHB promoter regions: 5'F/SD R and -35F/ATG...</p>
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<div>Yesterday's PCR products were run out on a gel (5'F/SD R, -35F/ATG R), which showed that the PCR was unsuccessful. <br />
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PCR was performed for two PHB promoter regions: 5'F/SD R and -35F/ATG R.</div>Trentmortensenhttp://2008.igem.org/Utah_State/24_October_2008Utah State/24 October 20082008-10-24T17:41:39Z<p>Trentmortensen: New page: Took Joseph's PCR products from last night: 1. 5'F/SD R 2. -35F/ATG R 3. phaB F/R 4. phaC F/phaB R E/S digested #'s 1 and 2 E/P digested #'s 3 and 4 Ran out on a gel Cut out from ge...</p>
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<div>Took Joseph's PCR products from last night: 1. 5'F/SD R 2. -35F/ATG R 3. phaB F/R 4. phaC F/phaB R<br />
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E/S digested #'s 1 and 2<br />
E/P digested #'s 3 and 4<br />
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Ran out on a gel<br />
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Cut out from gel and purified<br />
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Purified E0240 in pSB3K3 from culture.</div>Trentmortensenhttp://2008.igem.org/Utah_State/15_September_2008Utah State/15 September 20082008-10-17T01:31:55Z<p>Trentmortensen: New page: Eight PCR reactions performed: phbCfor/rev, phbCfor/phbBrev, 5'CproFor/ATGrev, 5'CproFor/SDrev, 5'CproFor/TCrev, -35CproFor/ATGrev, -35phafor/TCrev, and a negative control. After electro...</p>
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<div>Eight PCR reactions performed: phbCfor/rev, phbCfor/phbBrev, 5'CproFor/ATGrev, 5'CproFor/SDrev, 5'CproFor/TCrev, -35CproFor/ATGrev, -35phafor/TCrev, and a negative control. After electrophoresis, bands were cut out for the second, third, fourth, fifth, sixth and seventh reactions. See electrophoresis picture and loose white sheet labeled with date for documentation.</div>Trentmortensenhttp://2008.igem.org/Utah_State/10_October_2008Utah State/10 October 20082008-10-16T23:50:33Z<p>Trentmortensen: New page: Digested 9 samples of 4 biobricks E/P. Purified DNA of phbA in pSB3K3 and digested. Ran all samples on gel. Electrophoresis order in yellow book.</p>
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<div>Digested 9 samples of 4 biobricks E/P.<br />
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Purified DNA of phbA in pSB3K3 and digested.<br />
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Ran all samples on gel.<br />
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Electrophoresis order in yellow book.</div>Trentmortensenhttp://2008.igem.org/Utah_State/11_October_2008Utah State/11 October 20082008-10-16T23:46:51Z<p>Trentmortensen: New page: Restriction digest (E/P) was repeated on the 10 potential biobricks. They were reordered to put like biobricks next to each other: changed from: L,1,2,3,4,5,6,7,8,9,10,L to L,3,5,2,7,...</p>
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<div>Restriction digest (E/P) was repeated on the 10 potential biobricks. They were reordered to put like biobricks next to each other: changed from: L,1,2,3,4,5,6,7,8,9,10,L to L,3,5,2,7,8,6,4,1,9,10,L. It is possible that 2 ul EcoR1 was added to -35phbCproF/TCrev (9). Incubated for 1 hour at 37C.</div>Trentmortensenhttp://2008.igem.org/Utah_State/13_October_2008Utah State/13 October 20082008-10-16T23:41:23Z<p>Trentmortensen: </p>
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<div>1. E/P digested Joseph's PCR product for phbB (#12 of his samples, lane 15 of his electrophoresis picture).<br />
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2. Made cultures of 10 possible biobrick samples to troubleshoot causes for mixed results.</div>Trentmortensenhttp://2008.igem.org/Utah_State/13_October_2008Utah State/13 October 20082008-10-16T23:41:12Z<p>Trentmortensen: New page: 1. E/P digested Joseph's PCR product for phbB (#12 of his samples, lane 15 of his electrophoresis picture). 2. Make cultures of 10 possible biobrick samples to troubleshoot causes for m...</p>
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<div>1. E/P digested Joseph's PCR product for phbB (#12 of his samples, lane 15 of his electrophoresis picture).<br />
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2. Make cultures of 10 possible biobrick samples to troubleshoot causes for mixed results.</div>Trentmortensenhttp://2008.igem.org/Utah_State/16_October_2008Utah State/16 October 20082008-10-16T23:39:23Z<p>Trentmortensen: New page: Isolated 4 cultures of I13600, E/P digested (40 ul reactions), ran out on gel, cut our from gel (4 x pSB1A3). Performed PCR on 3 possible biobricks (#9, #4 and #7, #6 of 10 tubes of possi...</p>
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<div>Isolated 4 cultures of I13600, E/P digested (40 ul reactions), ran out on gel, cut our from gel (4 x pSB1A3).<br />
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Performed PCR on 3 possible biobricks (#9, #4 and #7, #6 of 10 tubes of possible biobricks) from 10/11/2008 electrophoresis pictures & ran out on gel to confirm - looks good! Cut out from gel just in case they would be needed.<br />
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Reinoculated all 10 potential biobricks because they were left in the freezer.<br />
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Transformed pSB3K3 with ccdb toxin into special competent cells.</div>Trentmortensenhttp://2008.igem.org/Utah_State/8_October_2008Utah State/8 October 20082008-10-08T23:15:50Z<p>Trentmortensen: New page: All liquid cultures from yesterday were turbid. 0.5 ml were saved for each possible biobrick for cryostocks. Plasmid DNA was isolated from the rest of each culture. Transformations were...</p>
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<div>All liquid cultures from yesterday were turbid. 0.5 ml were saved for each possible biobrick for cryostocks. Plasmid DNA was isolated from the rest of each culture. Transformations were performed from the ligations from 10/6 (phbA into pSB1A3, phbA into pSB3K3, phbB into pSB1A3, and phbB into pSB3K3). To conserve competent cells, 50 ul competent cells were used for all four transformations (12ul/transformation) and 2 ul DNA was added to each.</div>Trentmortensenhttp://2008.igem.org/Utah_State/6_October_2008Utah State/6 October 20082008-10-07T21:06:53Z<p>Trentmortensen: </p>
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<div>Ligations were attempted today for the phbA and phbB genes into the vectors pSB3K3 and pSB1A3. Transformations will be attempted Wednesday. Also, several ligations and transformations produced colonies from last Friday: E0240 into pSB3K3(L1 and L2), (5phbCpro For + ATG pha Cpro Rev) into pSB1A3 (B2), (5phbCpro For + TCphaCpro Rev) into pSB1A3 (B4), and (-35phaCpro For + TCphaCpro Rev) into pSB1A3 (B6).</div>Trentmortensenhttp://2008.igem.org/Utah_State/7_October_2008Utah State/7 October 20082008-10-07T21:04:02Z<p>Trentmortensen: New page: Liquid cultures were made from yesterday's colonies. Transformation will be done tomorrow. Trent</p>
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<div>Liquid cultures were made from yesterday's colonies. Transformation will be done tomorrow. <br />
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Trent</div>Trentmortensenhttp://2008.igem.org/Utah_State/3_October_2008Utah State/3 October 20082008-10-06T23:55:59Z<p>Trentmortensen: New page: Ligation attempts were made for E0240 into pSB3K3.</p>
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<div>Ligation attempts were made for E0240 into pSB3K3.</div>Trentmortensenhttp://2008.igem.org/Utah_State/6_October_2008Utah State/6 October 20082008-10-06T23:54:59Z<p>Trentmortensen: </p>
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<div>Ligations were attempted today for the phbA and phbB genes into the vectors pSB3K3 and pSB1A3. Transformations will be attempted Wednesday. Also, several ligations and transformations produced colonies from last Friday: E0240 into pSB3K3, (5phbCpro For + ATG pha Cpro Rev) into pSB1A3, (5phbCpro For + TCphaCpro Rev) into pSB1A3, and (-35phaCpro For + TCphaCpro Rev) into pSB1A3.</div>Trentmortensenhttp://2008.igem.org/Utah_State/6_October_2008Utah State/6 October 20082008-10-06T23:54:23Z<p>Trentmortensen: New page: Ligations were attempted today for the phbA and phbB genes into the vectors pSB3K3 and pSB1A3. Transformations will be attempted Wednesday. Also, several ligations produced colonies from...</p>
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<div>Ligations were attempted today for the phbA and phbB genes into the vectors pSB3K3 and pSB1A3. Transformations will be attempted Wednesday. Also, several ligations produced colonies from last Friday: E0240 into pSB3K3, (5phbCpro For + ATG pha Cpro Rev) into pSB1A3, (5phbCpro For + TCphaCpro Rev) into pSB1A3, and (-35phaCpro For + TCphaCpro Rev) into pSB1A3.</div>Trentmortensenhttp://2008.igem.org/Utah_State/19_September_2008Utah State/19 September 20082008-09-22T19:15:51Z<p>Trentmortensen: New page: Extracted various promoter regions from electrophoresis gel with Qiagen kit, namely C for-B rev, 5'Cpro for-ATG rev, 5'Cpro for-SD rev, 5'Cpro for-TC rev, -35Cpro for-ATG rev, and -35 pha ...</p>
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<div>Extracted various promoter regions from electrophoresis gel with Qiagen kit, namely C for-B rev, 5'Cpro for-ATG rev, 5'Cpro for-SD rev, 5'Cpro for-TC rev, -35Cpro for-ATG rev, and -35 pha for-TC C rev. Also divided presentation and reporting responsibilities for the Jamboree.</div>Trentmortensenhttp://2008.igem.org/Utah_State/22_September_2008Utah State/22 September 20082008-09-22T19:06:03Z<p>Trentmortensen: New page: Isolated plasmid DNA from I20260 and E0240 constructs.</p>
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<div>Isolated plasmid DNA from I20260 and E0240 constructs.</div>Trentmortensenhttp://2008.igem.org/File:Logo.jpgFile:Logo.jpg2008-09-19T20:30:29Z<p>Trentmortensen: uploaded a new version of "Image:Logo.jpg": Reverted to version as of 21:51, 1 July 2008</p>
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<div></div>Trentmortensenhttp://2008.igem.org/Utah_State/3_September_2008Utah State/3 September 20082008-09-03T23:50:45Z<p>Trentmortensen: New page: Linear DNA was extracted from electrophoresis gels for the following constructs: 1. phbA with prefix and suffix 2. phbB with p/s 3. -35 pro with p/s 4. -35/TC with p/s 5. phbB w/out ...</p>
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<div>Linear DNA was extracted from electrophoresis gels for the following constructs:<br />
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1. phbA with prefix and suffix<br />
2. phbB with p/s<br />
3. -35 pro with p/s<br />
4. -35/TC with p/s<br />
5. phbB w/out p/s<br />
6. E0240 cut with EcoR1 and Xba1<br />
7. E0840 "<br />
8. E0420 "<br />
9. E0422 "<br />
10. E0430 "<br />
11. p-E0240 "<br />
12. I712074 cut with Spe1 and Pst1<br />
13. R0010 "<br />
14. R0040 "<br />
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Also, transformations were performed for I6036 and J06650</div>Trentmortensenhttp://2008.igem.org/Utah_State/27_August_2008Utah State/27 August 20082008-08-27T22:40:59Z<p>Trentmortensen: New page: Restriction digestion and electrophoresis was performed for the following: With EcoR1 and Xba1: E0240, E0840, E0420, E0422, E0430, E0240 With Spe1 and Pst1: I712074, R0010, R0040</p>
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<div>Restriction digestion and electrophoresis was performed for the following:<br />
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With EcoR1 and Xba1: E0240, E0840, E0420, E0422, E0430, E0240<br />
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With Spe1 and Pst1: I712074, R0010, R0040</div>Trentmortensenhttp://2008.igem.org/Utah_State/15_August_2008Utah State/15 August 20082008-08-15T21:32:40Z<p>Trentmortensen: New page: PCR was run for 16s primers for the H16 cells and M. KMS cells, and Xba1 and Spe1 digestion was performed on portions of all purified DNA; electrophoresis was run for all of these.</p>
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<div>PCR was run for 16s primers for the H16 cells and M. KMS cells, and Xba1 and Spe1 digestion was performed on portions of all purified DNA; electrophoresis was run for all of these.</div>Trentmortensenhttp://2008.igem.org/Utah_State/14_August_2008Utah State/14 August 20082008-08-14T21:34:12Z<p>Trentmortensen: New page: DNA isolation was performed for the nine constructs that had not grown before. Plans were made for performing PCR with new primers tomorrow.</p>
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<div>DNA isolation was performed for the nine constructs that had not grown before. Plans were made for performing PCR with new primers tomorrow.</div>Trentmortensenhttp://2008.igem.org/Utah_State/13_August_2008Utah State/13 August 20082008-08-14T21:32:43Z<p>Trentmortensen: New page: Cultures were prepared for the nine cultures that did not grow.</p>
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<div>Cultures were prepared for the nine cultures that did not grow.</div>Trentmortensenhttp://2008.igem.org/Utah_State/12_August_2008Utah State/12 August 20082008-08-14T21:31:49Z<p>Trentmortensen: New page: DNA isolation was performed for several constructs, but cultures did not grow for nine of the constructs.</p>
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<div>DNA isolation was performed for several constructs, but cultures did not grow for nine of the constructs.</div>Trentmortensenhttp://2008.igem.org/Utah_State/11_August_2008Utah State/11 August 20082008-08-14T21:30:44Z<p>Trentmortensen: New page: Students learned how to use the DNA isolation kit. Cultures were prepared for all 22 transformed constructs for DNA isolation tomorrow.</p>
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<div>Students learned how to use the DNA isolation kit. Cultures were prepared for all 22 transformed constructs for DNA isolation tomorrow.</div>Trentmortensenhttp://2008.igem.org/Utah_State/6_August_2008Utah State/6 August 20082008-08-06T20:48:55Z<p>Trentmortensen: New page: Due to negative PCR results, it was decided to re-inoculate H16 Ralstonia Eutropha cultures since there may have been contamination in the culture used during the past two days of PCR. Si...</p>
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<div>Due to negative PCR results, it was decided to re-inoculate H16 Ralstonia Eutropha cultures since there may have been contamination in the culture used during the past two days of PCR. Six cultures were made from two glycerol stocks, one grown in LB media, and the other grown in TBS, a kind of limited media. PCR will be attempted again tomorrow.</div>Trentmortensenhttp://2008.igem.org/Utah_State/5_August_2008Utah State/5 August 20082008-08-06T20:45:24Z<p>Trentmortensen: New page: PCR was again attempted with the same oligos, but the annealing stage was performed on a gradient in the thermocycler. The gradient ranged from 40 to 60 C. Electrophoresis showed negativ...</p>
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<div>PCR was again attempted with the same oligos, but the annealing stage was performed on a gradient in the thermocycler. The gradient ranged from 40 to 60 C. Electrophoresis showed negative results for all attempts.</div>Trentmortensenhttp://2008.igem.org/Utah_State/4_August_2008Utah State/4 August 20082008-08-06T20:43:01Z<p>Trentmortensen: New page: PCR was done for the phb A,B, and C genes individually and as a whole. Amplification of the various regions of the promoter was also attempted. Electrophoresis showed negative results ex...</p>
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<div>PCR was done for the phb A,B, and C genes individually and as a whole. Amplification of the various regions of the promoter was also attempted. Electrophoresis showed negative results except in one of the promoter regions. PCR temperatures fluctuated from 94C to 60C to 72C.</div>Trentmortensenhttp://2008.igem.org/Utah_State/22_July_2008Utah State/22 July 20082008-07-23T19:50:45Z<p>Trentmortensen: New page: We discussed the process of making primers to PCR out genes of interest and how to submit BioBricks. We also looked at Biology Workbench and NCBI to learn the process of analyzing gene se...</p>
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<div>We discussed the process of making primers to PCR out genes of interest and how to submit BioBricks. We also looked at Biology Workbench and NCBI to learn the process of analyzing gene sequences in order to determine necessary mutations before submission as a BioBrick.</div>Trentmortensenhttp://2008.igem.org/Utah_State/18_July_2008Utah State/18 July 20082008-07-22T05:49:35Z<p>Trentmortensen: </p>
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<div>DNA separation preparation was done using the Mini-Scale Preparation for DNA Sequencing by CTAB-DNA Precipitation protocol. Restriction enzyme digestion was done using Xba1 and Pst1. Electrophoresis was then done on all 11 constructs from the last two days. Also, all protocols were written up in an organized form and consolidated.</div>Trentmortensenhttp://2008.igem.org/Utah_State/18_July_2008Utah State/18 July 20082008-07-19T01:58:08Z<p>Trentmortensen: New page: DNA separation preparation was done using the Mini-Scale Preparation for DNA Sequencing by DTAB-DNA Precipitation protocol. Restriction enzyme digestion was done using Xba1 and Pst1. Ele...</p>
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<div>DNA separation preparation was done using the Mini-Scale Preparation for DNA Sequencing by DTAB-DNA Precipitation protocol. Restriction enzyme digestion was done using Xba1 and Pst1. Electrophoresis was then done on all 11 constructs from the last two days. Also, all protocols were written up in an organized form and consolidated.</div>Trentmortensenhttp://2008.igem.org/Utah_State/17_July_2008Utah State/17 July 20082008-07-17T21:06:40Z<p>Trentmortensen: New page: Mini-scale preparation for DNA sequencing by CTAB-DNA precipitation was performed for all constructs except I20260 (the full GFP construct sent later by iGEM) up to the point of pellet rem...</p>
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<div>Mini-scale preparation for DNA sequencing by CTAB-DNA precipitation was performed for all constructs except I20260 (the full GFP construct sent later by iGEM) up to the point of pellet removal. Procedures were done to this point to facilitate doing electrophoresis tomorrow for all constructs. The I20260 transformation worked very well and will be brought to the same point as the other 10 constructs tomorrow, after which preparation for DNA sequencing, enzymatic digestion, and electrophoresis will be completed for all the constructs. Also, the I13521 (RFP) streak plate produced the characteristic red-hued bacteria, thereby showing the correct preparation of the antibiotic agar plates.</div>Trentmortensenhttp://2008.igem.org/Utah_State/16_July_2008Utah State/16 July 20082008-07-16T21:24:57Z<p>Trentmortensen: </p>
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<div>The I20260 construct was mistakenly placed on Amp plates. The transformation was redone today and placed on Kan plates. At least one colony per construct was noted (excepting I20260) and streak plates and liquid colonies were made for all constructs, two colonies if possible per construct. The E0240 construct from the newly sent pages from iGEM transformed the best by far. The test streak plate from yesterday (to see if the transformations were not successful because of the plates) grew fine, so the source of error from two days ago remains unknown.</div>Trentmortensenhttp://2008.igem.org/Utah_State/16_July_2008Utah State/16 July 20082008-07-16T21:22:21Z<p>Trentmortensen: New page: The I20260 construct was mistakenly placed on Amp plates. The transformation was redone today and placed on Kan plates. At least one colony per construct was noted (excepting I20260) and...</p>
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<div>The I20260 construct was mistakenly placed on Amp plates. The transformation was redone today and placed on Kan plates. At least one colony per construct was noted (excepting I20260) and streak plates and liquid colonies were made for all constructs, two colonies if possible per construct. The</div>Trentmortensenhttp://2008.igem.org/Utah_State/15_July_2008Utah State/15 July 20082008-07-15T16:55:59Z<p>Trentmortensen: New page: None of the eight transformations from yesterday worked. An ampicillin plate from the batch we made yesterday was streaked with previously successfully transformed with T7 luciferase E. c...</p>
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<div>None of the eight transformations from yesterday worked. An ampicillin plate from the batch we made yesterday was streaked with previously successfully transformed with T7 luciferase E. coli to see if the plate is the cause of failure. A new box of Top 10 competent cells were used yesterday, and that is also a possible source of problems. Today, the same transformations are being attempted again with the addition of the E0240 plasmid from the newer iGEM paper (should be the same as that from plate 1001-4B) that was mailed, a full GPF construct from that same paper - I20260, and the RFP - I13521 (previously successfully transformed) as a control.</div>Trentmortensenhttp://2008.igem.org/Utah_State/14_July_2008Utah State/14 July 20082008-07-15T16:47:54Z<p>Trentmortensen: New page: Today we attempted transformations for three promoters (T7-I712074, lac-R0010, and the CFP/RFP-R0040 promoters) and five promoterless reporters (GPF-E0240 and E0840, ECFP-E0420 and E0422, ...</p>
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<div>Today we attempted transformations for three promoters (T7-I712074, lac-R0010, and the CFP/RFP-R0040 promoters) and five promoterless reporters (GPF-E0240 and E0840, ECFP-E0420 and E0422, and EYFP-E0430).</div>Trentmortensenhttp://2008.igem.org/Utah_State/10_July_2008Utah State/10 July 20082008-07-10T16:27:11Z<p>Trentmortensen: </p>
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<div>The fluorescence of the GFP needing IPTG was tested. The sample without IPTG had an OD of 0.9 and the sample with IPTG had an OD of 0.7, not subtracting the media control. A fluorescence scan with excitation at 488 nm for a media control and the two samples showed only the two peaks (one at excitation and the other at a higher wavelength) characteristic of media, only varying in intensity due to light reflection from cells. The two streak plates, one with IPTG and one without, showed no visible fluorescence when placed on the UV transilluminator. At this point, it appears that the GPF transformation for J04430 is unsuccessful, although it may be possible that the concentration of IPTG is too low or needs to be added at a different point in time.</div>Trentmortensenhttp://2008.igem.org/Utah_State/10_July_2008Utah State/10 July 20082008-07-10T16:25:18Z<p>Trentmortensen: New page: The fluorescence of the GFP needing IPTG was tested. The sample without IPTG had an OD of 0.9 and the sample with IPTG had an OD of 0.7, not subtracting the media control. A fluorescence...</p>
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<div>The fluorescence of the GFP needing IPTG was tested. The sample without IPTG had an OD of 0.9 and the sample with IPTG had an OD of 0.7, not subtracting the media control. A fluorescence scan with excitation at 488 nm for a media control and the two samples showed only the two peaks (one at excitation and the other at a higher wavelength) characteristic of media, only varying in intensity due to light reflection from cells. The two streak plates, one with IPTG and one without, showed no visible fluorescence when placed on the UV transilluminator. At this point, it appears that the GPF transformation for J04430 is unsuccessful. It may be possible that the concentration of IPTG is too low or needs to be added at a different point in time.</div>Trentmortensenhttp://2008.igem.org/Utah_State/8_July_2008Utah State/8 July 20082008-07-09T20:41:44Z<p>Trentmortensen: New page: Restriction enzyme digest preparations were performed in order to do the digestion tomorrow for the three GFP strengths, the GFP needing IPTG to fluoresce, and the lux construct.</p>
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<div>Restriction enzyme digest preparations were performed in order to do the digestion tomorrow for the three GFP strengths, the GFP needing IPTG to fluoresce, and the lux construct.</div>Trentmortensenhttp://2008.igem.org/Utah_State/9_July_2008Utah State/9 July 20082008-07-09T20:38:59Z<p>Trentmortensen: New page: A restriction enzyme digest was performed with the three GFP constructs, the luciferase construct, and the GFP (needing IPTG). An electrophoresis gel was run. Colonies were prepared for ...</p>
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<div>A restriction enzyme digest was performed with the three GFP constructs, the luciferase construct, and the GFP (needing IPTG). An electrophoresis gel was run. Colonies were prepared for testing of the GPF construct needing IPTG. A rogue colony that had the appearance of GFP under UV was also streaked on a plate containing IPTG and one without as a control. Liquid cultures were also prepared, with and without IPTG. The GFP needing IPTG had a reddish hue. Fluorescence testing will be performed tomorrow.</div>Trentmortensenhttp://2008.igem.org/Utah_State/3_July_2008Utah State/3 July 20082008-07-03T17:29:41Z<p>Trentmortensen: New page: Fluorescence was tested for all of the GFP constructs. A notable difference was noted between the weak, medium, and strong promoter constructs. Excitation was 488 nm and emission tested ...</p>
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<div>Fluorescence was tested for all of the GFP constructs. A notable difference was noted between the weak, medium, and strong promoter constructs. Excitation was 488 nm and emission tested at 509 nm. A peak outside of the excitation peak was noted in the media alone, and further investigation needs to be done to determine the emission source of this peak. Fluorescence will be tested again next week to see if fluorescence changes during stationary phase.</div>Trentmortensenhttp://2008.igem.org/Utah_State/2_July_2008Utah State/2 July 20082008-07-03T17:25:46Z<p>Trentmortensen: New page: Colonies appeared for all transformations from yesterday. Liquid and streak plate cultures were created from these.</p>
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<div>Colonies appeared for all transformations from yesterday. Liquid and streak plate cultures were created from these.</div>Trentmortensenhttp://2008.igem.org/Utah_State/1_July_2008Utah State/1 July 20082008-07-01T21:56:16Z<p>Trentmortensen: New page: We retransformed the three GFP constructs sent to us from iGEM, namely the weak, medium, and strong promoter GFPs. We also transformed the GPF that was noted to work well after adding ITP...</p>
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<div>We retransformed the three GFP constructs sent to us from iGEM, namely the weak, medium, and strong promoter GFPs. We also transformed the GPF that was noted to work well after adding ITPG.</div>Trentmortensenhttp://2008.igem.org/File:Logo.jpgFile:Logo.jpg2008-07-01T21:51:48Z<p>Trentmortensen: </p>
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<div></div>Trentmortensenhttp://2008.igem.org/Team:Utah_StateTeam:Utah State2008-07-01T21:51:39Z<p>Trentmortensen: </p>
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<div><!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** --><br />
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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.<br />
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your Team:Example namespace. <br />
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|[[Image:Logo.jpg|200px|right|frame]]<br />
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|'''Utah State Project Description'''<br />
The increasing cost and negative environmental effect of fossil hydrocarbon-derived conventional plastics has escalated the need for economically realistic alternatives. Polyhydroxyalkanoates (PHAs) are a class of microbially synthesized, biodegradable thermoplastics that exhibit material properties comparable to those of conventional plastics. The Utah State University IGEM team project is focused on creating an efficient system for monitoring PHA production in recombinant microorganisms.<br />
|[[Image:Team.png|right|frame|Your team picture]]<br />
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|align="center"|[[Team:Utah_State | Team Example 2]]<br />
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:Utah_State|Home]]<br />
!align="center"|[[Team:Utah_State/Team|The Team]]<br />
!align="center"|[[Team:Utah_State/Project|The Project]]<br />
!align="center"|[[Team:Utah_State/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Utah_State/Modeling|Modeling]]<br />
!align="center"|[[Team:Utah_State/Notebook|Notebook]]<br />
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(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')</div>Trentmortensenhttp://2008.igem.org/File:Team_logo.jpgFile:Team logo.jpg2008-07-01T20:30:28Z<p>Trentmortensen: </p>
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<div></div>Trentmortensen