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From 2008.igem.org
Combined display of all available logs of 2008.igem.org. You can narrow down the view by selecting a log type, the user name (case-sensitive), or the affected page (also case-sensitive).- 01:01, 30 October 2008 Jrabl (Talk | contribs) uploaded "File:Jr rmf 4.jpg" (A Schematic representation of vector pET28a. Not that the vector has a constitutive LacI expression cassette. B BL21 DE3 cells holding the empty pET28a vector at 10mM IPTG. The vector is obviously nontoxic to cells even at high concentrations of inducer. )
- 00:51, 30 October 2008 Jrabl (Talk | contribs) uploaded a new version of "File:Jr gfpsubclone 1.jpg"
- 00:51, 30 October 2008 Jrabl (Talk | contribs) uploaded a new version of "File:Jr rmf 3.jpg"
- 00:48, 30 October 2008 Jrabl (Talk | contribs) uploaded "File:Jr pulsegen 5.jpg" (Pulse generator construct [http://partsregistry.org/wiki/index.php?title=Part:BBa_K142027 K142027] was produced from device [http://partsregistry.org/wiki/index.php?title=Part:BBa_K142047 K142047] using PCR-based site-directed mutagenesis as described pr)
- 13:07, 27 October 2008 Jrabl (Talk | contribs) uploaded "File:Jr pulsegen 4.jpg" (1% agarose gel of PCR products of site directed mutagenesis of the final pulse generator assembly consisting of [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:BBa_I763026 I763026] [http://partsregistry.org/wiki/index.php?ti)
- 12:19, 27 October 2008 Jrabl (Talk | contribs) uploaded "File:Jr gfpsubclone 1.jpg" (1% agarose gel of XbaI/PstI test restriction digests of plasmid [http://partsregistry.org/Part:pSB3K3 pSB3K3] holding fluorescent protein generators [http://partsregistry.org/Part:BBa_I763004 I763004], [http://partsregistry.org/Part:BBa_J04430 J04430], [h)
- 11:49, 27 October 2008 Jrabl (Talk | contribs) uploaded "File:Jr pulsegen 3.jpg" (A 1% agarose gel of XbaI/PstI test digests of plasmids holding the lac generator behind the tet-controlled promotor (RL1, RL2, RL3) and of XbaI/PstI test digests of plasmids holding the constitutive tetR expression cassette behind the promotorless lacI ge)
- 11:48, 27 October 2008 Jrabl (Talk | contribs) uploaded "File:Jr pulsegen 2.jpg" (A 1% agarose gel of PCR products of site directed mutagenesis of C0012. Template DNA of BioBrick [http://partsregistry.org/Part:BBa_C0012 C0012] was amplified by PCR in eight different reactions using primers 276F forward 5’-GGATACGACGATTTTGAAGACAGCTC; )
- 23:42, 26 October 2008 Jrabl (Talk | contribs) uploaded "File:Jr rmf 3.jpg" (Cell viability at increasing IPTG concentration. BL21 DE3 cells holding the pET28a-RMF plasmid with mutations R197A; R197F; T276A; T276F; R197A T276A; R197A T276F; R197F T276A; R197F T276F were plated on LB-agar with kanamycin without IPTG, with 1mM IPTG)
- 23:41, 26 October 2008 Jrabl (Talk | contribs) uploaded "File:Jr rmf 2.jpg" (‘’’A’’’ Growth curve of BL21 DE3 cells holding the pET28a-RMF plasmid without induction (uninduced) and after induction after 5 hours. Cells were grown in 1l of medium in 5l baffled flasks (37C, 95rpm) and induced once they had reached the exp)
- 23:40, 26 October 2008 Jrabl (Talk | contribs) uploaded "File:Jr rmf 1.jpg" (‘’’A’’’ 1.5% agarose gel with marker 100bp DNA ladder plus (Fermentas); pSB1A7: vector pSB1A7 digested with XbaI and SpeI according to http://www.neb.com NEB manual; RMF: PCR product of RMF amplified from DH5-alpha genomic DNA using forwar)
- 14:45, 26 October 2008 Jrabl (Talk | contribs) uploaded "File:Jr pulsegen 1.jpg" (A Lac repressor tetramer, residues R197 and T276 are shown in red. B IPTG bound to the inducer binding site of the lac repressor, residues R197 and T276 are shown in green. Molecular graphics was generated from coordinate set [http://www.rcsb.org/pdb/expl)
