TUDelft/19 August 2008

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(Testing the Primers)
(Testing the Primers)
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The PCR products were run on two different 1.5% agarose gels. Also a marker was run on the gel. The expected band sizes are ~550bp for IDI, ~900bp for ISPA and ~1200bp for ATOB. It is known that bands end up a little higher when running the SybrSafe product in the gel. The pictures are shown below:
The PCR products were run on two different 1.5% agarose gels. Also a marker was run on the gel. The expected band sizes are ~550bp for IDI, ~900bp for ISPA and ~1200bp for ATOB. It is known that bands end up a little higher when running the SybrSafe product in the gel. The pictures are shown below:
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[[Image:gelTUDelft1.jpg|thumb|left|Gel of IDI and ISPA, click picture to enlarge]]
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[[Image:gelTUDelft1.jpg|thumb|left|Gel of IDI and ISPA, click picture to enlarge]] [[Image:gelTUDelft2.jpg|thumb|right|Gel of ATOB, click picture to enlarge]]
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STILL NEED TO EDIT/PUT IN THE PICTURES
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==Live stabs==
==Live stabs==

Revision as of 09:05, 26 August 2008

19th August

Testing the Primers

The PCR products were run on two different 1.5% agarose gels. Also a marker was run on the gel. The expected band sizes are ~550bp for IDI, ~900bp for ISPA and ~1200bp for ATOB. It is known that bands end up a little higher when running the SybrSafe product in the gel. The pictures are shown below:

Gel of IDI and ISPA, click picture to enlarge
Gel of ATOB, click picture to enlarge

Live stabs

All the live stabs cultures have grown in 5 ml LB + A. We've made glycerol stocks, plated them and grown them in 50 ml LB medium + A o/n again to perform midiprep tomorrow.