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TUDelft/31 July 2008
From 2008.igem.org
[http://www.tudelft.nl ]
[http://www.tudelft.nl ]
Cells put into culture yesterday were used to inoculate 200ml of LB. This was grown to an OD of 0.4. This was used to make glycerol stocks (60% glycerol was used, the protocol is on OpenWetWare). Not all 200ml was used for this, the surplus was discarded.
