August 7th

Transformations

Some transformations were tried again. This time our own DB3.1 Top10 competent cells were used, they were transformed with the DNA of the first spot (well 4H of source plate 1004, inducible GFP) and another one (well 1B source plate 1020, inducible luciferase expression). Furthermore, a positive control (pUC18, isolated on 25-07-2008) and a negative control (competent cells without plasmid on ampicillin plate) were performed. The protocol on OpenWetWare was used again. Cells were incubated o/n @ 37oC.