Team:BCCS-Bristol/Calendar-Notebook/1 July 2008

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This is a page for any solutions
This is a page for any solutions
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SOC medium in preparation for Biobrick transformation this consisted of
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Trypton'''
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| align="center" style="background:#f0f0f0;"|'''2g'''
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|-
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| Yeast Extract||0.5g
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|-
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| NaCl||0.05g
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|-
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|
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|}
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in 95ml of water, then autoclaved. after this add this:
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''MgCl2'''
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| align="center" style="background:#f0f0f0;"|'''0.5ml 2M solution'''
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|-
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| Glucose||2ml 1M
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|-
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|
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|}
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then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.

Revision as of 11:46, 24 July 2008

This is a page for any solutions

SOC medium in preparation for Biobrick transformation this consisted of

Trypton 2g
Yeast Extract0.5g
NaCl0.05g

in 95ml of water, then autoclaved. after this add this:

MgCl2 0.5ml 2M solution
Glucose2ml 1M

then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.

Retrieved from "http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/1_July_2008"