Team:Beijing Normal/Notebook

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<!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** -->
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{| style="color:#ffffff;background-color:#6699cc;" cellpadding="3" cellspacing="1" border="0" bordercolor="#fff" align="center" width="80%"
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!align="center"|[[Team:Beijing_Normal|Home]]
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!align="center"|[[Team:Beijing_Normal/Team|The Team]]
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!align="center"|[[Team:Beijing_Normal/Project|The Project]]
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!align="center"|[[Team:Beijing_Normal/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Beijing_Normal/Notebook|Notebook]]
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=='''Dynamics'''==
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<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
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*== '''Communications''' ==
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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**17th June:       Attend the Asian Workshop meeting.
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This is a template page. READ THESE INSTRUCTIONS.
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your Team:Example namespace.
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**17th July:        Meet up with Tsinghua Team, and learn fron their experiences.
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**25th July:        With Invitrogen.Co. Ltd and Tsinghua team to sign up a contract.
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**27th July:        Have a discussion with Tsinghua team about the transformation efficiency.
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We generalize some key words from the discussion:
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{|align="justify"
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1. Make sure the concentration of the plasmids from the biobrick is enough for transformation.  
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|You can write a background of your team here. Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Example_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Team.png|right|frame|Your team picture]]
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|align="center"|[[Team:Beijing_Normal | Team Example 2]]
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<!--- The Mission, Experiments --->
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2. Keep a low temprature when enzyme digestion is performed
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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3. The transformation product are always incubated for more than 1h.
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!align="center"|[[Team:Beijing_Normal|Home]]
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!align="center"|[[Team:Beijing_Normal/Team|The Team]]
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!align="center"|[[Team:Beijing_Normal/Project|The Project]]
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**9th September:   We and Tsinghua team get together in our lab. We have a happy time together and share a lot.
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!align="center"|[[Team:Beijing_Normal/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Beijing_Normal/Modeling|Modeling]]
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**10th September:  We provide Tsinghua Team with the cells harboring pSB1AC3 plasmids. We did the the tramsformation for them. Because they found their transformation effeciency is relatively low.
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!align="center"|[[Team:Beijing_Normal/Notebook|Notebook]]
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|}
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**17th September: Tsinghua Team told us that the vector that we provided proved to work well. We were all pretty happy about this positive result.
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(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')
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**3th October:      We and Chiba Team had a happy conversation via googletalk today. We share all the success and frustrations in the process. And we exchange our opinion about promotor function measurement. They told us that they have used BBa_T9002 because their project uses quorum sensing and the part(T9002) was GFP producer controlled by 3OC6HSL Receiver Device.And their result is pretty satisfying.
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**5th October:      We share the experience of measurment with Tokyo Tech University Team. They tell us to use BBa_I13522 (http://partsregistry.org/Part:BBa_I13522) as a positive control when we measure the function of promotors.
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**9th October:      We test the founction of Part BBa_I719015. The result indicates that it works well. The cells (BL21(DE3) as host) turn  green when induced by IPTG.
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**15th October:      Our T-shirt is designed today. It is very pretty. See image below.
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**19th, 20th October:  We have a good discussion with USTC team in Hefei and they give us several important suggestions on experiments.
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[[Image:t-shirt.jpg]]
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[[Image:t-shirt1.jpg]]
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'''Our logo is designed finally. It is a visual field through a microscope. You could see us in this microscope.'''
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[[Image:our logo.jpg]]
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** Our iGem is coming to the end. But our projet will continue.
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Of course, we encountered many obstacles and difficulties such as financial problems, cooperation and communication problems of teammates, spirit pressure, time management and so on. But all the hardship turned out to be a precious experience that helped us grow up. Anyway, iGem is one of our most precious experiences and we will never forget these days and night in the lab and meeting room, all the laughs and tears. We have learned a lot in this project except the attending the competition itself.
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==Notebook==
 
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You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well. 
 
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Find more information on how to use the calendar feature by going to the [[Help:Calendar | general calendar page]].
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{{ #calendar: title=Beijing_Normal |year=2008|month=07}}
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{{ #calendar: title=Beijing_Normal |year=2008|month=08}}
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{{ #calendar: title=Beijing_Normal |year=2008|month=09}}
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{{ #calendar: title=Beijing_Normal |year=2008|month=10}}
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{{ #calendar: title=Beijing_Normal |year=2008|month=11}}

Latest revision as of 04:02, 30 October 2008

Home The Team The Project Parts Submitted to the Registry Notebook

Dynamics

  • == Communications ==
    • 17th June: Attend the Asian Workshop meeting.
    • 17th July: Meet up with Tsinghua Team, and learn fron their experiences.
    • 25th July: With Invitrogen.Co. Ltd and Tsinghua team to sign up a contract.
    • 27th July: Have a discussion with Tsinghua team about the transformation efficiency.

We generalize some key words from the discussion:

1. Make sure the concentration of the plasmids from the biobrick is enough for transformation.

2. Keep a low temprature when enzyme digestion is performed

3. The transformation product are always incubated for more than 1h.


    • 9th September: We and Tsinghua team get together in our lab. We have a happy time together and share a lot.
    • 10th September: We provide Tsinghua Team with the cells harboring pSB1AC3 plasmids. We did the the tramsformation for them. Because they found their transformation effeciency is relatively low.
    • 17th September: Tsinghua Team told us that the vector that we provided proved to work well. We were all pretty happy about this positive result.
    • 3th October: We and Chiba Team had a happy conversation via googletalk today. We share all the success and frustrations in the process. And we exchange our opinion about promotor function measurement. They told us that they have used BBa_T9002 because their project uses quorum sensing and the part(T9002) was GFP producer controlled by 3OC6HSL Receiver Device.And their result is pretty satisfying.
    • 5th October: We share the experience of measurment with Tokyo Tech University Team. They tell us to use BBa_I13522 (http://partsregistry.org/Part:BBa_I13522) as a positive control when we measure the function of promotors.
    • 9th October: We test the founction of Part BBa_I719015. The result indicates that it works well. The cells (BL21(DE3) as host) turn green when induced by IPTG.
    • 15th October: Our T-shirt is designed today. It is very pretty. See image below.
    • 19th, 20th October: We have a good discussion with USTC team in Hefei and they give us several important suggestions on experiments.

T-shirt.jpg

T-shirt1.jpg

Our logo is designed finally. It is a visual field through a microscope. You could see us in this microscope.

Our logo.jpg

    • Our iGem is coming to the end. But our projet will continue.

Of course, we encountered many obstacles and difficulties such as financial problems, cooperation and communication problems of teammates, spirit pressure, time management and so on. But all the hardship turned out to be a precious experience that helped us grow up. Anyway, iGem is one of our most precious experiences and we will never forget these days and night in the lab and meeting room, all the laughs and tears. We have learned a lot in this project except the attending the competition itself.



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