Team:Bologna/Parts

From 2008.igem.org

(Difference between revisions)
Line 55: Line 55:
| Output
| Output
| Discarded
| Discarded
-
|-
+
|- style="background:#f6efcd; color:grey"
| '''K145001'''
| '''K145001'''
| /
| /
Line 62: Line 62:
| ''(nowhere)''
| ''(nowhere)''
| Discarded
| Discarded
-
|- style="background:#e0ffff; color:grey"
+
|- style="background:#f6efcd; color:grey"
| '''K145002'''
| '''K145002'''
| /
| /
Line 69: Line 69:
| ''(nowhere)''
| ''(nowhere)''
| Discarded
| Discarded
-
|- style="background:#e0ffff; color:grey"
+
|- style="background:#f6efcd; color:grey"
| '''K145003'''
| '''K145003'''
| '''K145102'''
| '''K145102'''
Line 76: Line 76:
| ''(nowhere)''
| ''(nowhere)''
| Discarded
| Discarded
-
|- style="background:#e0ffff; color:grey"
+
|- style="background:#f6efcd; color:grey"
| '''K145004'''
| '''K145004'''
| '''K145104'''
| '''K145104'''
Line 83: Line 83:
| Inverter
| Inverter
| Discarded
| Discarded
-
|- style="background:#e0ffff; color:grey"
+
|- style="background:#f6efcd; color:grey"
| '''K145005'''
| '''K145005'''
| /
| /
Line 90: Line 90:
| Pulse generator
| Pulse generator
| Discarded
| Discarded
-
|- style="background:#e0ffff; color:grey"
+
|- style="background:#f6efcd; color:grey"
| '''K145006'''
| '''K145006'''
| '''K145106'''
| '''K145106'''
Line 97: Line 97:
| ''(nowhere)''
| ''(nowhere)''
| Discarded
| Discarded
-
|- style="background:#e0ffff; color:grey"
+
|- style="background:#f6efcd; color:grey"
| '''K145007'''
| '''K145007'''
| '''K145107'''
| '''K145107'''
Line 105: Line 105:
| Discarded
| Discarded
|- style="background:#e0ffff; color:grey"
|- style="background:#e0ffff; color:grey"
-
| '''K145008'''
+
|
-
| '''K145108'''
+
|
-
| Constitutive production of LuxR
+
|
-
| Composite part
+
|
-
| ''(nowhere)''
+
|
-
| Discarded
+
|
|- style="background:#e0ffff; color:grey"
|- style="background:#e0ffff; color:grey"
-
| '''K145009'''
+
|
-
| '''K145109'''
+
|
-
| ccdB cell death gene under control of an activating Lux PR
+
|
-
| Composite part
+
|
-
| ''(nowhere)''
+
|
-
| Discarded
+
||- style="background:#e0ffff; color:grey"
-
|- style="background:#e0ffff; color:grey"
+
|
-
| '''K145010'''
+
|
-
| '''K145110'''
+
|
-
| Killing switch
+
|
-
| Composite part
+
|
-
| ''(nowhere)''
+
||- style="background:#e0ffff; color:grey"
-
| Discarded
+
|
-
|- style="background:#e0ffff; color:grey"
+
|
-
| '''K145011'''
+
|
-
| /
+
|
-
| Light sensor to provide dummy input for the system, copy of '''BBa_M30109'''
+
|
-
| Composite part
+
||- style="background:#e0ffff; color:grey"
-
| Input
+
|
-
| Deleted
+
|
-
|- style="background:#e0ffff; color:grey"
+
|
-
| '''K145012'''
+
|
-
| /
+
|
-
| N-terminal degradation tag
+
||- style="background:#e0ffff; color:grey"
-
| Part
+
|
-
| Filter
+
|
-
| Not used
+
|
-
|- style="background:#e0ffff; color:black"
+
|
-
| '''K145013'''
+
|
-
| /
+
||- style="background:#e0ffff; color:grey"
-
| antisense LuxI
+
|
-
| Part
+
|
-
| Memory
+
|
-
| In use
+
|
-
|- style="background:#e0ffff; color:black"
+
|
-
| '''K145014'''
+
||- style="background:#e0ffff; color:grey"
-
| /
+
|
-
| T7 RNA Polymerase with N-terminal UmuD degradation tag
+
|
-
| Part
+
|
-
| Filter
+
|
-
| In use
+
|
-
|- style="background:#e0ffff; color:black"
+
||- style="background:#e0ffff; color:grey"
-
| '''K145015'''
+
|
-
| /
+
|
-
| GFP with LVA tag
+
|
-
| Part
+
|
-
| Ouput
+
|
-
| In use
+
||- style="background:#e0ffff; color:grey"
-
|- style="background:#e0ffff; color:black"
+
|
-
| '''K145016'''
+
|
-
| /
+
|
-
| Intermediate TetR-generator
+
|
-
| Composite part
+
|
-
| Input
+
||- style="background:#e0ffff; color:grey"
-
| In use
+
|
-
|- style="background:#e0ffff; color:grey"
+
|
-
| '''K145100'''
+
|
-
| '''K145205'''
+
|
-
| OmpF PoPS -> GFP
+
|
-
| Composite part
+
||- style="background:#e0ffff; color:grey"
-
| Output
+
|
-
| Discarded
+
|
-
|- style="background:#e0ffff; color:grey"
+
|
-
| '''K145101'''
+
|
-
| '''K145110'''
+
|
-
| OmpF PoPS -> deactivate constitutive antisense LuxI
+
||- style="background:#e0ffff; color:grey"
-
| Composite part
+
|
-
| Memory
+
|
-
| Discarded
+
|
-
|- style="background:#e0ffff; color:grey"
+
|
-
| '''K145102'''
+
|
-
| '''K145215'''
+
||- style="background:#e0ffff; color:grey"
-
| OmpF PoPS -> RiboKey3d
+
|
-
| Composite part
+
|
-
| Filter
+
|
-
| Discarded
+
|
-
|- style="background:#e0ffff; color:grey"
+
|
-
| '''K145103'''
+
||- style="background:#e0ffff; color:grey"
-
| '''K145216'''
+
|
-
| T7 with UmuD derived tag under constitutive promotor + RiboLock3d
+
|
-
| Composite Part
+
|
-
| Filter
+
|
-
| Discarded
+
|
-
|- style="background:#e0ffff; color:grey"
+
||- style="background:#e0ffff; color:grey"
-
| '''K145104'''
+
|
-
| '''K145220'''
+
|
-
| T7 PoPS + RiboLock -> LacI -> inhibition of LuxI
+
|
-
| Composite part
+
|
-
| Inverter
+
|
-
| Discarded
+
||- style="background:#e0ffff; color:grey"
-
|- style="background:#e0ffff; color:grey"
+
|
-
| '''K145105'''
+
|
-
| '''K145225'''
+
|
-
| cI under T7 and P<sub>R</sub> dual promotor
+
|
-
| Composite part
+
|
-
| Pulse Generator
+
||- style="background:#e0ffff; color:grey"
-
| Discarded
+
|
-
|- style="background:#e0ffff; color:black"
+
|
-
| '''K145106'''
+
|
-
| /
+
|
-
| cI P<sub>RM</sub> PoPS -> Lactonase
+
|
-
| Composite part
+
||- style="background:#e0ffff; color:grey"
-
| Pulse Generator
+
|
-
| Unknown
+
|
-
|- style="background:#e0ffff; color:grey"
+
|
-
| '''K145107'''
+
|
-
| '''K145117'''
+
|
-
| Lactonase pulse generator
+
||- style="background:#e0ffff; color:grey"
-
| Composite part
+
|
-
| Pulse Generator
+
|
-
| Discarded
+
|
-
|- style="background:#e0ffff; color:black"
+
|
-
| '''K145108'''
+
|
-
| /
+
||- style="background:#e0ffff; color:grey"
-
| Constitutive PoPS -> LuxR
+
|
-
| Composite part
+
|
-
| Cell death
+
|
-
| Unknown
+
|
-
|- style="background:#e0ffff; color:black"
+
|
-
| '''K145109'''
+
||- style="background:#e0ffff; color:grey"
-
| /
+
|
-
| Hybrid P22 c2 - luxP<sub>R</sub> PoPS -> ccdB
+
|
-
| Composite part
+
|
-
| Cell death
+
|
-
| Unknown
+
|
-
|- style="background:#e0ffff; color:black"
+
||- style="background:#e0ffff; color:black"
-
| '''K145110'''
+
-
| /
+
-
| Cell death mechanism
+
-
| Composite part
+
-
| Cell death
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145111'''
+
-
| /
+
-
| OmpF PoPS -> deactivate constitutive antisense LuxI
+
-
| Composite part
+
-
| Memory
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145112'''
+
-
| /
+
-
| cI under T7 and PR<sub>R</sub> dual promotor
+
-
| Composite part
+
-
| Pulse generator
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145113'''
+
-
| /
+
-
| Constitutive PoPS -> TetR
+
-
| ?
+
-
| ?
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145115'''
+
-
| /
+
-
| cI under T7 and PRR dual promotor
+
-
| Composite part
+
-
| Pulse generator
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145117'''
+
-
| /
+
-
| Lactonase pulse generator
+
-
| Composite part
+
-
| Pulse generator
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145150'''
+
-
| /
+
-
| Hybrid promoter: HSL-LuxR activated, P22 C2 repressed
+
-
| Part
+
-
| CellDeath
+
-
| In use
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145151'''
+
-
| /
+
-
| ccdB coding region
+
-
| Part
+
-
| CellDeath
+
-
| In use
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145152'''
+
-
| /
+
-
| Hybrid promoter: P22 c2 , LacI NOR gate
+
-
| Part
+
-
| ?
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145200'''
+
-
| /
+
-
| INPUT lightsensor (DISCARDED)
+
-
| Composite Part
+
-
| Input
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145201'''
+
-
| /
+
-
| INPUT TetR generator
+
-
| Composite Part
+
-
| INPUT
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145205'''
+
-
| /
+
-
| OUTPUT GFP under TetR pomoter
+
-
| Composite Part
+
-
| OUTPUT
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145210'''
+
-
| /
+
-
| MEMORY
+
-
| Composite Part
+
-
| MEMORY
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145211'''
+
-
| /
+
-
| MEMORY OUTPUT asLuxI
+
-
| Composite Part
+
-
| MEMORY
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145215'''
+
-
| /
+
-
| FILTER Key
+
-
| Composite Part
+
-
| FILTER
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145216'''
+
-
| /
+
-
| FILTER T7 RNA pol
+
-
| Composite Part
+
-
| FILTER
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145217'''
+
-
| /
+
-
| FILTER Complete
+
-
| Composite Part
+
-
| FILTER
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145220'''
+
-
| /
+
-
| INVERTED TIMER
+
-
| Composite Part
+
-
| INVERTIMER
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
-
| '''K145225'''
+
-
| /
+
-
| RESET lactonase
+
-
| Composite Part
+
-
| RESET
+
-
| Unknown
+
-
|- style="background:#e0ffff; color:black"
+
| '''K145230'''
| '''K145230'''
| /
| /

Revision as of 08:44, 2 October 2008

Logo1a.gifTestata dx.jpg
HOME THE PROJECT THE TEAM PARTS SUBMITTED TO THE REGISTRY MODELING NOTEBOOK


Intermediates

The table below shows all the intermediates that we have obtained to build up our final device. For each part you can find:

-in the first column the link to the Registry;

-in the second column the components for each part;

-in the third column the plasmid the part has been cloned in;

-in the fourth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the fifth column cell vitality after transformation;

-in the sixth column fluo data for each part.


Part number Replaced by Description Type Component Status
K145000 K145100 eCFP with LVA tag under OmpR control Part Output Discarded
K145001 / T7 RNA polymerase Part (nowhere) Discarded
K145002 / T7 RNA Polymerase under OmpF control Composite part (nowhere) Discarded
K145003 K145102 RiboKey 3d under control of T7 RNA polymerase promotor Composite part (nowhere) Discarded
K145004 K145104 T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac. Composite part Inverter Discarded
K145005 / cI under control of a dual promotor and a RiboLock. Composite part Pulse generator Discarded
K145006 K145106 Lactonase under PRM control Composite part (nowhere) Discarded
K145007 K145107 Pulse Generator Composite part (nowhere) Discarded
- style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:grey" - style="background:#e0ffff; color:black" K145230 / CELL DEATH Composite Part CELLDEATH Unknown
Retrieved from "http://2008.igem.org/Team:Bologna/Parts"