Team:Bologna/Parts
From 2008.igem.org
(Difference between revisions)
Line 68: | Line 68: | ||
| Part | | Part | ||
| Output | | Output | ||
- | | Discarded|- style="background:#f6efcd; color:grey" | + | | Discarded |
+ | |- style="background:#f6efcd; color:grey" | ||
| '''K145000''' | | '''K145000''' | ||
| '''K145100''' | | '''K145100''' | ||
Line 74: | Line 75: | ||
| Part | | Part | ||
| Output | | Output | ||
- | | Discarded|- style="background:# | + | | Discarded |
- | | ''' | + | |- style="background:#e0ffff; color:grey" |
- | | ''' | + | | '''K145004''' |
- | | | + | | '''K145104''' |
+ | | T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac. | ||
+ | | Composite part | ||
+ | | Inverter | ||
+ | | Discarded | ||
+ | |- style="background:#e0ffff; color:grey" | ||
+ | | '''K145005''' | ||
+ | | / | ||
+ | | cI under control of a dual promotor and a RiboLock. | ||
+ | | Composite part | ||
+ | | Pulse generator | ||
+ | | Discarded | ||
+ | |- style="background:#e0ffff; color:grey" | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |- style="background:#e0ffff; color:grey" | ||
+ | | '''K145007''' | ||
+ | | '''K145107''' | ||
+ | | Pulse Generator | ||
+ | | Composite part | ||
+ | | ''(nowhere)'' | ||
+ | | Discarded | ||
+ | |- style="background:#e0ffff; color:grey" | ||
+ | | '''K145008''' | ||
+ | | '''K145108''' | ||
+ | | Constitutive production of LuxR | ||
+ | | Composite part | ||
+ | | ''(nowhere)'' | ||
+ | | Discarded | ||
+ | |- style="background:#e0ffff; color:grey" | ||
+ | | '''K145009''' | ||
+ | | '''K145109''' | ||
+ | | ccdB cell death gene under control of an activating Lux PR | ||
+ | | Composite part | ||
+ | | ''(nowhere)'' | ||
+ | | Discarded | ||
+ | |- style="background:#e0ffff; color:grey" | ||
+ | | '''K145010''' | ||
+ | | '''K145110''' | ||
+ | | Killing switch | ||
+ | | Composite part | ||
+ | | ''(nowhere)'' | ||
+ | | Discarded | ||
+ | |- style="background:#e0ffff; color:grey" | ||
+ | | '''K145011''' | ||
+ | | / | ||
+ | | Light sensor to provide dummy input for the system, copy of '''BBa_M30109''' | ||
+ | | Composite part | ||
+ | | Input | ||
+ | | Deleted | ||
+ | |- style="background:#e0ffff; color:grey" | ||
+ | | '''K145012''' | ||
+ | | / | ||
+ | | N-terminal degradation tag | ||
| Part | | Part | ||
- | | | + | | Filter |
- | | | + | | Not used |
- | | ''' | + | |- style="background:#e0ffff; color:black" |
- | | | + | | '''K145013''' |
- | | | + | | / |
+ | | antisense LuxI | ||
| Part | | Part | ||
- | | | + | | Memory |
- | | | + | | In use |
- | | ''' | + | |- style="background:#e0ffff; color:black" |
- | | | + | | '''K145014''' |
- | | | + | | / |
+ | | T7 RNA Polymerase with N-terminal UmuD degradation tag | ||
| Part | | Part | ||
- | | | + | | Filter |
- | | | + | | In use |
- | | ''' | + | |- style="background:#e0ffff; color:black" |
- | | | + | | '''K145015''' |
- | | | + | | / |
+ | | GFP with LVA tag | ||
| Part | | Part | ||
- | | | + | | Ouput |
- | | | + | | In use |
- | | ''' | + | |- style="background:#e0ffff; color:black" |
+ | | '''K145016''' | ||
+ | | / | ||
+ | | Intermediate TetR-generator | ||
+ | | Composite part | ||
+ | | Input | ||
+ | | In use | ||
+ | |- style="background:#e0ffff; color:grey" | ||
| '''K145100''' | | '''K145100''' | ||
- | | | + | | '''K145205''' |
- | | | + | | OmpF PoPS -> GFP |
+ | | Composite part | ||
| Output | | Output | ||
- | | Discarded|- style="background:# | + | | Discarded |
- | | ''' | + | |- style="background:#e0ffff; color:grey" |
- | | ''' | + | | '''K145101''' |
- | | | + | | '''K145110''' |
+ | | OmpF PoPS -> deactivate constitutive antisense LuxI | ||
+ | | Composite part | ||
+ | | Memory | ||
+ | | Discarded | ||
+ | |- style="background:#e0ffff; color:grey" | ||
+ | | '''K145102''' | ||
+ | | '''K145215''' | ||
+ | | OmpF PoPS -> RiboKey3d | ||
+ | | Composite part | ||
+ | | Filter | ||
+ | | Discarded | ||
+ | |- style="background:#e0ffff; color:grey" | ||
+ | | '''K145103''' | ||
+ | | '''K145216''' | ||
+ | | T7 with UmuD derived tag under constitutive promotor + RiboLock3d | ||
+ | | Composite Part | ||
+ | | Filter | ||
+ | | Discarded | ||
+ | |- style="background:#e0ffff; color:grey" | ||
+ | | '''K145104''' | ||
+ | | '''K145220''' | ||
+ | | T7 PoPS + RiboLock -> LacI -> inhibition of LuxI | ||
+ | | Composite part | ||
+ | | Inverter | ||
+ | | Discarded | ||
+ | |- style="background:#e0ffff; color:grey" | ||
+ | | '''K145105''' | ||
+ | | '''K145225''' | ||
+ | | cI under T7 and P<sub>R</sub> dual promotor | ||
+ | | Composite part | ||
+ | | Pulse Generator | ||
+ | | Discarded | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145106''' | ||
+ | | / | ||
+ | | cI P<sub>RM</sub> PoPS -> Lactonase | ||
+ | | Composite part | ||
+ | | Pulse Generator | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:grey" | ||
+ | | '''K145107''' | ||
+ | | '''K145117''' | ||
+ | | Lactonase pulse generator | ||
+ | | Composite part | ||
+ | | Pulse Generator | ||
+ | | Discarded | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145108''' | ||
+ | | / | ||
+ | | Constitutive PoPS -> LuxR | ||
+ | | Composite part | ||
+ | | Cell death | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145109''' | ||
+ | | / | ||
+ | | Hybrid P22 c2 - luxP<sub>R</sub> PoPS -> ccdB | ||
+ | | Composite part | ||
+ | | Cell death | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145110''' | ||
+ | | / | ||
+ | | Cell death mechanism | ||
+ | | Composite part | ||
+ | | Cell death | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145111''' | ||
+ | | / | ||
+ | | OmpF PoPS -> deactivate constitutive antisense LuxI | ||
+ | | Composite part | ||
+ | | Memory | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145112''' | ||
+ | | / | ||
+ | | cI under T7 and PR<sub>R</sub> dual promotor | ||
+ | | Composite part | ||
+ | | Pulse generator | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145113''' | ||
+ | | / | ||
+ | | Constitutive PoPS -> TetR | ||
+ | | ? | ||
+ | | ? | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145115''' | ||
+ | | / | ||
+ | | cI under T7 and PRR dual promotor | ||
+ | | Composite part | ||
+ | | Pulse generator | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145117''' | ||
+ | | / | ||
+ | | Lactonase pulse generator | ||
+ | | Composite part | ||
+ | | Pulse generator | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145150''' | ||
+ | | / | ||
+ | | Hybrid promoter: HSL-LuxR activated, P22 C2 repressed | ||
| Part | | Part | ||
- | | | + | | CellDeath |
- | | | + | | In use |
- | | ''' | + | |- style="background:#e0ffff; color:black" |
- | | | + | | '''K145151''' |
- | | | + | | / |
+ | | ccdB coding region | ||
| Part | | Part | ||
- | | | + | | CellDeath |
- | | | + | | In use |
- | | ''' | + | |- style="background:#e0ffff; color:black" |
- | | | + | | '''K145152''' |
- | | | + | | / |
+ | | Hybrid promoter: P22 c2 , LacI NOR gate | ||
| Part | | Part | ||
- | | | + | | ? |
- | | | + | | Unknown |
- | | ''' | + | |- style="background:#e0ffff; color:black" |
- | | ''' | + | | '''K145200''' |
- | | | + | | / |
- | | Part | + | | INPUT lightsensor (DISCARDED) |
- | | | + | | Composite Part |
- | | | + | | Input |
- | | ''' | + | | Unknown |
- | | ''' | + | |- style="background:#e0ffff; color:black" |
- | | | + | | '''K145201''' |
- | | Part | + | | / |
- | | | + | | INPUT TetR generator |
- | | | + | | Composite Part |
- | | ''' | + | | INPUT |
- | | ''' | + | | Unknown |
- | | | + | |- style="background:#e0ffff; color:black" |
- | | Part | + | | '''K145205''' |
- | | | + | | / |
- | | | + | | OUTPUT GFP under TetR pomoter |
- | | ''' | + | | Composite Part |
- | | ''' | + | | OUTPUT |
- | | | + | | Unknown |
- | | Part | + | |- style="background:#e0ffff; color:black" |
- | | | + | | '''K145210''' |
- | | | + | | / |
- | | ''' | + | | MEMORY |
- | | ''' | + | | Composite Part |
- | | | + | | MEMORY |
- | | Part | + | | Unknown |
- | | | + | |- style="background:#e0ffff; color:black" |
- | | | + | | '''K145211''' |
+ | | / | ||
+ | | MEMORY OUTPUT asLuxI | ||
+ | | Composite Part | ||
+ | | MEMORY | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145215''' | ||
+ | | / | ||
+ | | FILTER Key | ||
+ | | Composite Part | ||
+ | | FILTER | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145216''' | ||
+ | | / | ||
+ | | FILTER T7 RNA pol | ||
+ | | Composite Part | ||
+ | | FILTER | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145217''' | ||
+ | | / | ||
+ | | FILTER Complete | ||
+ | | Composite Part | ||
+ | | FILTER | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145220''' | ||
+ | | / | ||
+ | | INVERTED TIMER | ||
+ | | Composite Part | ||
+ | | INVERTIMER | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145225''' | ||
+ | | / | ||
+ | | RESET lactonase | ||
+ | | Composite Part | ||
+ | | RESET | ||
+ | | Unknown | ||
+ | |- style="background:#e0ffff; color:black" | ||
+ | | '''K145230''' | ||
+ | | / | ||
+ | | CELL DEATH | ||
+ | | Composite Part | ||
+ | | CELLDEATH | ||
+ | | Unknown | ||
|} | |} |
Revision as of 08:49, 2 October 2008
HOME | THE PROJECT | THE TEAM | PARTS SUBMITTED TO THE REGISTRY | MODELING | NOTEBOOK |
---|
Intermediates
The table below shows all the intermediates that we have obtained to build up our final device. For each part you can find:
-in the first column the link to the Registry;
-in the second column the components for each part;
-in the third column the plasmid the part has been cloned in;
-in the fourth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;
-in the fifth column cell vitality after transformation;
-in the sixth column fluo data for each part.
Part number | Replaced by | Description | Type | Component | Status |
K145000 | K145100 | eCFP with LVA tag under OmpR control | Part | Output | Discarded |
K145000 | K145100 | eCFP with LVA tag under OmpR control | Part | Output | Discarded |
K145000 | K145100 | eCFP with LVA tag under OmpR control | Part | Output | Discarded |
K145000 | K145100 | eCFP with LVA tag under OmpR control | Part | Output | Discarded |
K145004 | K145104 | T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac. | Composite part | Inverter | Discarded |
K145005 | / | cI under control of a dual promotor and a RiboLock. | Composite part | Pulse generator | Discarded |
K145007 | K145107 | Pulse Generator | Composite part | (nowhere) | Discarded |
K145008 | K145108 | Constitutive production of LuxR | Composite part | (nowhere) | Discarded |
K145009 | K145109 | ccdB cell death gene under control of an activating Lux PR | Composite part | (nowhere) | Discarded |
K145010 | K145110 | Killing switch | Composite part | (nowhere) | Discarded |
K145011 | / | Light sensor to provide dummy input for the system, copy of BBa_M30109 | Composite part | Input | Deleted |
K145012 | / | N-terminal degradation tag | Part | Filter | Not used |
K145013 | / | antisense LuxI | Part | Memory | In use |
K145014 | / | T7 RNA Polymerase with N-terminal UmuD degradation tag | Part | Filter | In use |
K145015 | / | GFP with LVA tag | Part | Ouput | In use |
K145016 | / | Intermediate TetR-generator | Composite part | Input | In use |
K145100 | K145205 | OmpF PoPS -> GFP | Composite part | Output | Discarded |
K145101 | K145110 | OmpF PoPS -> deactivate constitutive antisense LuxI | Composite part | Memory | Discarded |
K145102 | K145215 | OmpF PoPS -> RiboKey3d | Composite part | Filter | Discarded |
K145103 | K145216 | T7 with UmuD derived tag under constitutive promotor + RiboLock3d | Composite Part | Filter | Discarded |
K145104 | K145220 | T7 PoPS + RiboLock -> LacI -> inhibition of LuxI | Composite part | Inverter | Discarded |
K145105 | K145225 | cI under T7 and PR dual promotor | Composite part | Pulse Generator | Discarded |
K145106 | / | cI PRM PoPS -> Lactonase | Composite part | Pulse Generator | Unknown |
K145107 | K145117 | Lactonase pulse generator | Composite part | Pulse Generator | Discarded |
K145108 | / | Constitutive PoPS -> LuxR | Composite part | Cell death | Unknown |
K145109 | / | Hybrid P22 c2 - luxPR PoPS -> ccdB | Composite part | Cell death | Unknown |
K145110 | / | Cell death mechanism | Composite part | Cell death | Unknown |
K145111 | / | OmpF PoPS -> deactivate constitutive antisense LuxI | Composite part | Memory | Unknown |
K145112 | / | cI under T7 and PRR dual promotor | Composite part | Pulse generator | Unknown |
K145113 | / | Constitutive PoPS -> TetR | ? | ? | Unknown |
K145115 | / | cI under T7 and PRR dual promotor | Composite part | Pulse generator | Unknown |
K145117 | / | Lactonase pulse generator | Composite part | Pulse generator | Unknown |
K145150 | / | Hybrid promoter: HSL-LuxR activated, P22 C2 repressed | Part | CellDeath | In use |
K145151 | / | ccdB coding region | Part | CellDeath | In use |
K145152 | / | Hybrid promoter: P22 c2 , LacI NOR gate | Part | ? | Unknown |
K145200 | / | INPUT lightsensor (DISCARDED) | Composite Part | Input | Unknown |
K145201 | / | INPUT TetR generator | Composite Part | INPUT | Unknown |
K145205 | / | OUTPUT GFP under TetR pomoter | Composite Part | OUTPUT | Unknown |
K145210 | / | MEMORY | Composite Part | MEMORY | Unknown |
K145211 | / | MEMORY OUTPUT asLuxI | Composite Part | MEMORY | Unknown |
K145215 | / | FILTER Key | Composite Part | FILTER | Unknown |
K145216 | / | FILTER T7 RNA pol | Composite Part | FILTER | Unknown |
K145217 | / | FILTER Complete | Composite Part | FILTER | Unknown |
K145220 | / | INVERTED TIMER | Composite Part | INVERTIMER | Unknown |
K145225 | / | RESET lactonase | Composite Part | RESET | Unknown |
K145230 | / | CELL DEATH | Composite Part | CELLDEATH | Unknown |