|
|
Line 76: |
Line 76: |
| | Output | | | Output |
| | Discarded | | | Discarded |
- | |- style="background:#e0ffff; color:grey" | + | |- style="background:#f6efcd; color:grey" |
- | | '''K145004''' | + | | '''K145000''' |
- | | '''K145104''' | + | | '''K145100''' |
- | | T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac. | + | | eCFP with LVA tag under OmpR control |
- | | Composite part | + | | Part |
- | | Inverter | + | | Output |
| | Discarded | | | Discarded |
- | |- style="background:#e0ffff; color:grey" | + | |- style="background:#f6efcd; color:grey" |
- | | '''K145005''' | + | | '''K145000''' |
- | | / | + | | '''K145100''' |
- | | cI under control of a dual promotor and a RiboLock. | + | | eCFP with LVA tag under OmpR control |
- | | Composite part | + | | Part |
- | | Pulse generator | + | | Output |
| | Discarded | | | Discarded |
- | |- style="background:#e0ffff; color:grey" | + | |- style="background:#f6efcd; color:grey" |
- | |
| + | | '''K145000''' |
- | |
| + | | '''K145100''' |
- | |
| + | | eCFP with LVA tag under OmpR control |
- | |
| + | | Part |
- | |
| + | | Output |
- | |
| + | |
- | |- style="background:#e0ffff; color:grey"
| + | |
- | | '''K145007''' | + | |
- | | '''K145107''' | + | |
- | | Pulse Generator | + | |
- | | Composite part | + | |
- | | ''(nowhere)'' | + | |
| | Discarded | | | Discarded |
- | |- style="background:#e0ffff; color:grey" | + | |- style="background:#f6efcd; color:grey" |
- | | '''K145008''' | + | | '''K145000''' |
- | | '''K145108''' | + | | '''K145100''' |
- | | Constitutive production of LuxR | + | | eCFP with LVA tag under OmpR control |
- | | Composite part | + | | Part |
- | | ''(nowhere)'' | + | | Output |
| | Discarded | | | Discarded |
- | |- style="background:#e0ffff; color:grey" | + | |- style="background:#f6efcd; color:grey" |
- | | '''K145009''' | + | | '''K145000''' |
- | | '''K145109''' | + | | '''K145100''' |
- | | ccdB cell death gene under control of an activating Lux PR | + | | eCFP with LVA tag under OmpR control |
- | | Composite part
| + | |
- | | ''(nowhere)''
| + | |
- | | Discarded
| + | |
- | |- style="background:#e0ffff; color:grey"
| + | |
- | | '''K145010'''
| + | |
- | | '''K145110'''
| + | |
- | | Killing switch
| + | |
- | | Composite part
| + | |
- | | ''(nowhere)''
| + | |
- | | Discarded
| + | |
- | |- style="background:#e0ffff; color:grey"
| + | |
- | | '''K145011'''
| + | |
- | | /
| + | |
- | | Light sensor to provide dummy input for the system, copy of '''BBa_M30109'''
| + | |
- | | Composite part
| + | |
- | | Input
| + | |
- | | Deleted
| + | |
- | |- style="background:#e0ffff; color:grey"
| + | |
- | | '''K145012'''
| + | |
- | | /
| + | |
- | | N-terminal degradation tag
| + | |
| | Part | | | Part |
- | | Filter
| |
- | | Not used
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145013'''
| |
- | | /
| |
- | | antisense LuxI
| |
- | | Part
| |
- | | Memory
| |
- | | In use
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145014'''
| |
- | | /
| |
- | | T7 RNA Polymerase with N-terminal UmuD degradation tag
| |
- | | Part
| |
- | | Filter
| |
- | | In use
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145015'''
| |
- | | /
| |
- | | GFP with LVA tag
| |
- | | Part
| |
- | | Ouput
| |
- | | In use
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145016'''
| |
- | | /
| |
- | | Intermediate TetR-generator
| |
- | | Composite part
| |
- | | Input
| |
- | | In use
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145100'''
| |
- | | '''K145205'''
| |
- | | OmpF PoPS -> GFP
| |
- | | Composite part
| |
| | Output | | | Output |
| | Discarded | | | Discarded |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145101'''
| |
- | | '''K145110'''
| |
- | | OmpF PoPS -> deactivate constitutive antisense LuxI
| |
- | | Composite part
| |
- | | Memory
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145102'''
| |
- | | '''K145215'''
| |
- | | OmpF PoPS -> RiboKey3d
| |
- | | Composite part
| |
- | | Filter
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145103'''
| |
- | | '''K145216'''
| |
- | | T7 with UmuD derived tag under constitutive promotor + RiboLock3d
| |
- | | Composite Part
| |
- | | Filter
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145104'''
| |
- | | '''K145220'''
| |
- | | T7 PoPS + RiboLock -> LacI -> inhibition of LuxI
| |
- | | Composite part
| |
- | | Inverter
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145105'''
| |
- | | '''K145225'''
| |
- | | cI under T7 and P<sub>R</sub> dual promotor
| |
- | | Composite part
| |
- | | Pulse Generator
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145106'''
| |
- | | /
| |
- | | cI P<sub>RM</sub> PoPS -> Lactonase
| |
- | | Composite part
| |
- | | Pulse Generator
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145107'''
| |
- | | '''K145117'''
| |
- | | Lactonase pulse generator
| |
- | | Composite part
| |
- | | Pulse Generator
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145108'''
| |
- | | /
| |
- | | Constitutive PoPS -> LuxR
| |
- | | Composite part
| |
- | | Cell death
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145109'''
| |
- | | /
| |
- | | Hybrid P22 c2 - luxP<sub>R</sub> PoPS -> ccdB
| |
- | | Composite part
| |
- | | Cell death
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145110'''
| |
- | | /
| |
- | | Cell death mechanism
| |
- | | Composite part
| |
- | | Cell death
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145111'''
| |
- | | /
| |
- | | OmpF PoPS -> deactivate constitutive antisense LuxI
| |
- | | Composite part
| |
- | | Memory
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145112'''
| |
- | | /
| |
- | | cI under T7 and PR<sub>R</sub> dual promotor
| |
- | | Composite part
| |
- | | Pulse generator
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145113'''
| |
- | | /
| |
- | | Constitutive PoPS -> TetR
| |
- | | ?
| |
- | | ?
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145115'''
| |
- | | /
| |
- | | cI under T7 and PRR dual promotor
| |
- | | Composite part
| |
- | | Pulse generator
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145117'''
| |
- | | /
| |
- | | Lactonase pulse generator
| |
- | | Composite part
| |
- | | Pulse generator
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145150'''
| |
- | | /
| |
- | | Hybrid promoter: HSL-LuxR activated, P22 C2 repressed
| |
- | | Part
| |
- | | CellDeath
| |
- | | In use
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145151'''
| |
- | | /
| |
- | | ccdB coding region
| |
- | | Part
| |
- | | CellDeath
| |
- | | In use
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145152'''
| |
- | | /
| |
- | | Hybrid promoter: P22 c2 , LacI NOR gate
| |
- | | Part
| |
- | | ?
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145200'''
| |
- | | /
| |
- | | INPUT lightsensor (DISCARDED)
| |
- | | Composite Part
| |
- | | Input
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145201'''
| |
- | | /
| |
- | | INPUT TetR generator
| |
- | | Composite Part
| |
- | | INPUT
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145205'''
| |
- | | /
| |
- | | OUTPUT GFP under TetR pomoter
| |
- | | Composite Part
| |
- | | OUTPUT
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145210'''
| |
- | | /
| |
- | | MEMORY
| |
- | | Composite Part
| |
- | | MEMORY
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145211'''
| |
- | | /
| |
- | | MEMORY OUTPUT asLuxI
| |
- | | Composite Part
| |
- | | MEMORY
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145215'''
| |
- | | /
| |
- | | FILTER Key
| |
- | | Composite Part
| |
- | | FILTER
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145216'''
| |
- | | /
| |
- | | FILTER T7 RNA pol
| |
- | | Composite Part
| |
- | | FILTER
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145217'''
| |
- | | /
| |
- | | FILTER Complete
| |
- | | Composite Part
| |
- | | FILTER
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145220'''
| |
- | | /
| |
- | | INVERTED TIMER
| |
- | | Composite Part
| |
- | | INVERTIMER
| |
- | | Unknown
| |
| |- style="background:#e0ffff; color:black" | | |- style="background:#e0ffff; color:black" |
| | '''K145225''' | | | '''K145225''' |
The table below shows all the intermediates that we have obtained to build up our final device. For each part you can find:
-in the fourth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;
-in the sixth column fluo data for each part.