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Team:Bologna/Parts
From 2008.igem.org
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| - | |width= | + | |width=8%| '''Number''' |
| - | |width= | + | |width=15%| '''Igem Code''' |
| - | |width= | + | |width=40%| '''Fragment''' |
| - | |width= | + | |width=15%| '''Plasmid''' |
| - | |width= | + | |width=15%| '''Length (bp)''' |
| - | |width= | + | |width=7%| '''Vitality''' |
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Revision as of 09:50, 2 October 2008
| HOME | THE PROJECT | THE TEAM | PARTS SUBMITTED TO THE REGISTRY | MODELING | NOTEBOOK |
|---|
Intermediates
The table below shows all the intermediates that we have obtained to build up our final device. For each part you can find:
-in the first column the link to the Registry;
-in the second column the components for each part;
-in the third column the plasmid the part has been cloned in;
-in the fourth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;
-in the fifth column cell vitality after transformation;
-in the sixth column fluo data for each part.
| Number | Igem Code | Fragment | Plasmid | Length (bp) | Vitality |
| K145007 | |||||
| K145004 | K145104 | T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac. | Composite part | Inverter | Discarded |
| K145005 | / | cI under control of a dual promotor and a RiboLock. | Composite part | Pulse generator | Discarded |
| K145007 | K145107 | Pulse Generator | Composite part | (nowhere) | Discarded |
| K145008 | K145108 | Constitutive production of LuxR | Composite part | (nowhere) | Discarded |
| K145009 | K145109 | ccdB cell death gene under control of an activating Lux PR | Composite part | (nowhere) | Discarded |
| K145010 | K145110 | Killing switch | Composite part | (nowhere) | Discarded |
| K145011 | / | Light sensor to provide dummy input for the system, copy of BBa_M30109 | Composite part | Input | Deleted |
| K145012 | / | N-terminal degradation tag | Part | Filter | Not used |
| K145013 | / | antisense LuxI | Part | Memory | In use |
| K145014 | / | T7 RNA Polymerase with N-terminal UmuD degradation tag | Part | Filter | In use |
| K145015 | / | GFP with LVA tag | Part | Ouput | In use |
| K145016 | / | Intermediate TetR-generator | Composite part | Input | In use |
| K145100 | K145205 | OmpF PoPS -> GFP | Composite part | Output | Discarded |
| K145101 | K145110 | OmpF PoPS -> deactivate constitutive antisense LuxI | Composite part | Memory | Discarded |
| K145102 | K145215 | OmpF PoPS -> RiboKey3d | Composite part | Filter | Discarded |
| K145103 | K145216 | T7 with UmuD derived tag under constitutive promotor + RiboLock3d | Composite Part | Filter | Discarded |
| K145104 | K145220 | T7 PoPS + RiboLock -> LacI -> inhibition of LuxI | Composite part | Inverter | Discarded |
| K145105 | K145225 | cI under T7 and PR dual promotor | Composite part | Pulse Generator | Discarded |
| K145106 | / | cI PRM PoPS -> Lactonase | Composite part | Pulse Generator | Unknown |
| K145107 | K145117 | Lactonase pulse generator | Composite part | Pulse Generator | Discarded |
| K145108 | / | Constitutive PoPS -> LuxR | Composite part | Cell death | Unknown |
| K145109 | / | Hybrid P22 c2 - luxPR PoPS -> ccdB | Composite part | Cell death | Unknown |
| K145110 | / | Cell death mechanism | Composite part | Cell death | Unknown |
| K145111 | / | OmpF PoPS -> deactivate constitutive antisense LuxI | Composite part | Memory | Unknown |
| K145112 | / | cI under T7 and PRR dual promotor | Composite part | Pulse generator | Unknown |
| K145113 | / | Constitutive PoPS -> TetR | ? | ? | Unknown |
| K145115 | / | cI under T7 and PRR dual promotor | Composite part | Pulse generator | Unknown |
| K145117 | / | Lactonase pulse generator | Composite part | Pulse generator | Unknown |
| K145150 | / | Hybrid promoter: HSL-LuxR activated, P22 C2 repressed | Part | CellDeath | In use |
| K145151 | / | ccdB coding region | Part | CellDeath | In use |
| K145152 | / | Hybrid promoter: P22 c2 , LacI NOR gate | Part | ? | Unknown |
| K145200 | / | INPUT lightsensor (DISCARDED) | Composite Part | Input | Unknown |
| K145201 | / | INPUT TetR generator | Composite Part | INPUT | Unknown |
| K145205 | / | OUTPUT GFP under TetR pomoter | Composite Part | OUTPUT | Unknown |
| K145210 | / | MEMORY | Composite Part | MEMORY | Unknown |
| K145211 | / | MEMORY OUTPUT asLuxI | Composite Part | MEMORY | Unknown |
| K145215 | / | FILTER Key | Composite Part | FILTER | Unknown |
| K145216 | / | FILTER T7 RNA pol | Composite Part | FILTER | Unknown |
| K145217 | / | FILTER Complete | Composite Part | FILTER | Unknown |
| K145220 | / | INVERTED TIMER | Composite Part | INVERTIMER | Unknown |
| K145225 | / | RESET lactonase | Composite Part | RESET | Unknown |
| K145230 | / | CELL DEATH | Composite Part | CELLDEATH | Unknown |
