Team:Bologna/Parts

From 2008.igem.org

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Revision as of 10:10, 27 October 2008

Logo1a.gifTestata dx.jpg
HOME TEAM PROJECT MODELING WET-LAB SOFTWARE SUBMITTED PARTS BIOSAFETY AND PROTOCOLS


Composites

The table below shows all the composites that we have obtained to build up our final device. For each part you can find:

-in the second column the link to the Registry;

-in the third column the components for each part;

-in the fourth column the plasmid the part has been cloned in;

-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the sixth column cell vitality after transformation;

-in the seventh column fluo data for each part.


N° Igem Code Fragment Plasmid Length (bp) Vitality Fluo
1
 K0790015 LacY transporter protein from E. coli pSB1AK3(HC) 1254
Yes
-
2
 K079017 Lac symmetric - operator library member pSB1AK3(HC) 20
Yes
3
 K079018 Lac 1 - operator library member pSB1A2(HC) 21
Yes
-
4
 K079019 Lac 2 - operator library member pSB1A2(HC) 21
Yes
5
 K079020 LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La pSB1AK3(HC) 2165
Yes
6
 K079021 LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La pSB1AK3(HC) 2165
Yes
-
7
 K079036 Tet O operator library member pSB1A2(HC) 15
Yes
8
 K079037 TetO-4C - operator library member pSB1A2(HC) 15
Yes
-
9
 K079038 TetO-wt/4C5G - operator library member pSB1AK3(HC) 15
Yes
-
10
 K079009 CI-RBS-GFP-T pSB1AK3(HC) 1697
Yes
11
 K079010 RBS-CI-T pSB1A2(HC) 930
Yes
-
12
 K079011 RBS-CI-RBS-GFP-T pSB1A2(HC) 1715
Yes
13
 O1Lac
-
14
 O2Lac
-
15
 O3Lac
-
16
 O1Tet
-
17
 O2Tet
-
18
 O3Tet
-
19
 O1Lambda
-
20
 O2Lambda
-
21
 O3Lambda
-
22
 O1LexA
-
23
 O2LexA
-
24
 O3LexA
-
25
 O2Lac-O1Lac
-
26
 O2Tet-O1Tet
-
27
 O2Lambda-O1Lambda
-
28
 O2LexA-O1LexA
-
29
 O3Lac-O2Lac-O1Lac
-
30
 O3Tet-O2Tet-O1Tet
-
31
 O3Lambda-O2Lambda-O1Lambda
-
32
 O3LexA-O2LexA-O1LexA
-
33
 O1Lac-RBS-LacI-T
-
34
 O2Lac-RBS-LacI-T
-
35
 O3Lac-RBS-LacI-T
-
36
 O1Lac-RBS-TetR-RBS-GFP-T
-
37
 O2Lac-RBS-TetR-RBS-GFP-T
-
38
 O3Lac-RBS-TetR-RBS-GFP-T
-


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