Team:Bologna/Parts

From 2008.igem.org

(Difference between revisions)
(→Composites)
(→Composites)
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|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>10</center>
| <center>10</center>
-
| [http://partsregistry.org/Part:BBa_K079009 K079009]
+
| [http://partsregistry.org/Part:BBa_K079039 K079039]
-
| CI-RBS-GFP-T
+
| LexA 1 - operator library member
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)]
-
| 1697
+
| 16
| <center>Yes</center>
| <center>Yes</center>
|  
|  
|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>11</center>
| <center>11</center>
-
| [http://partsregistry.org/Part:BBa_K079010 K079010]
+
| [http://partsregistry.org/Part:BBa_K079040 K079040]
| RBS-CI-T
| RBS-CI-T
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
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|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>12</center>
| <center>12</center>
-
| [http://partsregistry.org/Part:BBa_K079011 K079011]
+
| [http://partsregistry.org/Part:BBa_K079041 K079041]
| RBS-CI-RBS-GFP-T
| RBS-CI-RBS-GFP-T
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
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|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>13</center>
| <center>13</center>
-
|  
+
| [http://partsregistry.org/Part:BBa_K079042 K079042]
| O1Lac
| O1Lac
|  
|  
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|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>14</center>
| <center>14</center>
-
|  
+
| [http://partsregistry.org/Part:BBa_K079043 K079043]
| O2Lac
| O2Lac
|  
|  
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|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>15</center>
| <center>15</center>
-
|  
+
| [http://partsregistry.org/Part:BBa_K079045 K079045]
| O3Lac
| O3Lac
|  
|  
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|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>16</center>
| <center>16</center>
-
|  
+
| [http://partsregistry.org/Part:BBa_K079046 K079046]
| O1Tet
| O1Tet
|  
|  
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|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>17</center>
| <center>17</center>
-
|  
+
| [http://partsregistry.org/Part:BBa_K079047 K079047]
| O2Tet
| O2Tet
|  
|  
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|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>18</center>
| <center>18</center>
-
|  
+
| [http://partsregistry.org/Part:BBa_K079048 K079048]
| O3Tet
| O3Tet
|  
|  
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|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>19</center>
| <center>19</center>
-
|  
+
| [http://partsregistry.org/Part:BBa_K079049 K079049]
| O1Lambda
| O1Lambda
|  
|  
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|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>20</center>
| <center>20</center>
-
|  
+
| [http://partsregistry.org/Part:BBa_K079050 K079050]
| O2Lambda
| O2Lambda
|  
|  

Revision as of 10:14, 27 October 2008

Logo1a.gifTestata dx.jpg
HOME TEAM PROJECT MODELING WET-LAB SOFTWARE SUBMITTED PARTS BIOSAFETY AND PROTOCOLS


Composites

The table below shows all the composites that we have obtained to build up our final device. For each part you can find:

-in the second column the link to the Registry;

-in the third column the components for each part;

-in the fourth column the plasmid the part has been cloned in;

-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the sixth column cell vitality after transformation;

-in the seventh column fluo data for each part.


N° Igem Code Fragment Plasmid Length (bp) Vitality Fluo
1
 K0790015 LacY transporter protein from E. coli pSB1AK3(HC) 1254
Yes
-
2
 K079017 Lac symmetric - operator library member pSB1AK3(HC) 20
Yes
3
 K079018 Lac 1 - operator library member pSB1A2(HC) 21
Yes
-
4
 K079019 Lac 2 - operator library member pSB1A2(HC) 21
Yes
5
 K079020 LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La pSB1AK3(HC) 2165
Yes
6
 K079021 LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La pSB1AK3(HC) 2165
Yes
-
7
 K079036 Tet O operator library member pSB1A2(HC) 15
Yes
8
 K079037 TetO-4C - operator library member pSB1A2(HC) 15
Yes
-
9
 K079038 TetO-wt/4C5G - operator library member pSB1AK3(HC) 15
Yes
-
10
 K079039 LexA 1 - operator library member pSB1AK3(HC) 16
Yes
11
 K079040 RBS-CI-T pSB1A2(HC) 930
Yes
-
12
 K079041 RBS-CI-RBS-GFP-T pSB1A2(HC) 1715
Yes
13
 K079042 O1Lac
-
14
 K079043 O2Lac
-
15
 K079045 O3Lac
-
16
 K079046 O1Tet
-
17
 K079047 O2Tet
-
18
 K079048 O3Tet
-
19
 K079049 O1Lambda
-
20
 K079050 O2Lambda
-
21
 O3Lambda
-
22
 O1LexA
-
23
 O2LexA
-
24
 O3LexA
-
25
 O2Lac-O1Lac
-
26
 O2Tet-O1Tet
-
27
 O2Lambda-O1Lambda
-
28
 O2LexA-O1LexA
-
29
 O3Lac-O2Lac-O1Lac
-
30
 O3Tet-O2Tet-O1Tet
-
31
 O3Lambda-O2Lambda-O1Lambda
-
32
 O3LexA-O2LexA-O1LexA
-
33
 O1Lac-RBS-LacI-T
-
34
 O2Lac-RBS-LacI-T
-
35
 O3Lac-RBS-LacI-T
-
36
 O1Lac-RBS-TetR-RBS-GFP-T
-
37
 O2Lac-RBS-TetR-RBS-GFP-T
-
38
 O3Lac-RBS-TetR-RBS-GFP-T
-


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