Team:Bologna/Parts

From 2008.igem.org

(Difference between revisions)
(→Composites)
(→Composites)
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| <center>11</center>
| <center>11</center>
| [http://partsregistry.org/Part:BBa_K079040 K079040]
| [http://partsregistry.org/Part:BBa_K079040 K079040]
-
| RBS-CI-T
+
| LexA 2 - opeartor library member
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
-
| 930
+
| 16
| <center>Yes</center>
| <center>Yes</center>
| <center>-</center>
| <center>-</center>
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| <center>12</center>
| <center>12</center>
| [http://partsregistry.org/Part:BBa_K079041 K079041]
| [http://partsregistry.org/Part:BBa_K079041 K079041]
-
| RBS-CI-RBS-GFP-T
+
| Lambda OR1 - operator library membe
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
-
| 1715
+
| 17
| <center>Yes</center>
| <center>Yes</center>
-
|  
+
| <center>-</center>
|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>13</center>
| <center>13</center>
| [http://partsregistry.org/Part:BBa_K079042 K079042]
| [http://partsregistry.org/Part:BBa_K079042 K079042]
-
| O1Lac
+
| Lambda OR2 - operator library member
-
|
+
|  
|  
 +
| 17
| <center></center>
| <center></center>
| <center>-</center>
| <center>-</center>
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| <center>14</center>
| <center>14</center>
| [http://partsregistry.org/Part:BBa_K079043 K079043]
| [http://partsregistry.org/Part:BBa_K079043 K079043]
-
| O2Lac
+
| Lambda OR3 - operator library membe
-
|
+
|  
|  
 +
| 17
| <center></center>
| <center></center>
| <center>-</center>
| <center>-</center>
Line 163: Line 163:
| <center>15</center>
| <center>15</center>
| [http://partsregistry.org/Part:BBa_K079045 K079045]
| [http://partsregistry.org/Part:BBa_K079045 K079045]
-
| O3Lac
+
| Lac operator library
-
|
+
|  
|  
 +
| 78
| <center></center>
| <center></center>
| <center>-</center>
| <center>-</center>
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| <center>16</center>
| <center>16</center>
| [http://partsregistry.org/Part:BBa_K079046 K079046]
| [http://partsregistry.org/Part:BBa_K079046 K079046]
-
| O1Tet
+
| Tet operator library
-
|
+
|  
|  
 +
| 61
| <center></center>
| <center></center>
| <center>-</center>
| <center>-</center>
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| <center>17</center>
| <center>17</center>
| [http://partsregistry.org/Part:BBa_K079047 K079047]
| [http://partsregistry.org/Part:BBa_K079047 K079047]
-
| O2Tet
+
| Lambda operator library
-
|
+
|  
|  
 +
| 67
| <center></center>
| <center></center>
| <center>-</center>
| <center>-</center>
Line 187: Line 187:
| <center>18</center>
| <center>18</center>
| [http://partsregistry.org/Part:BBa_K079048 K079048]
| [http://partsregistry.org/Part:BBa_K079048 K079048]
-
| O3Tet
+
| LexA operator library
-
|
+
|  
|  
 +
| 40
| <center></center>
| <center></center>
| <center>-</center>
| <center>-</center>
Line 195: Line 195:
| <center>19</center>
| <center>19</center>
| [http://partsregistry.org/Part:BBa_K079049 K079049]
| [http://partsregistry.org/Part:BBa_K079049 K079049]
-
| O1Lambda
+
| GFP reporter protein under the control of the J23118 costitutive promoter and LexA 2 operator
-
|
+
|  
|  
 +
| 981
| <center></center>
| <center></center>
| <center>-</center>
| <center>-</center>
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| <center>20</center>
| <center>20</center>
| [http://partsregistry.org/Part:BBa_K079050 K079050]
| [http://partsregistry.org/Part:BBa_K079050 K079050]
-
| O2Lambda
+
| GFP reporter protein under the control of the J23100 costitutive promoter and LexA 2 operator
-
|
+
|  
|  
 +
| 981
| <center></center>
| <center></center>
| <center>-</center>
| <center>-</center>

Revision as of 10:24, 27 October 2008

Logo1a.gifTestata dx.jpg
HOME TEAM PROJECT MODELING WET-LAB SOFTWARE SUBMITTED PARTS BIOSAFETY AND PROTOCOLS


Composites

The table below shows all the composites that we have obtained to build up our final device. For each part you can find:

-in the second column the link to the Registry;

-in the third column the components for each part;

-in the fourth column the plasmid the part has been cloned in;

-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the sixth column cell vitality after transformation;

-in the seventh column fluo data for each part.


N° Igem Code Fragment Plasmid Length (bp) Vitality Fluo
1
 K0790015 LacY transporter protein from E. coli pSB1AK3(HC) 1254
Yes
-
2
 K079017 Lac symmetric - operator library member pSB1AK3(HC) 20
Yes
3
 K079018 Lac 1 - operator library member pSB1A2(HC) 21
Yes
-
4
 K079019 Lac 2 - operator library member pSB1A2(HC) 21
Yes
5
 K079020 LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La pSB1AK3(HC) 2165
Yes
6
 K079021 LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La pSB1AK3(HC) 2165
Yes
-
7
 K079036 Tet O operator library member pSB1A2(HC) 15
Yes
8
 K079037 TetO-4C - operator library member pSB1A2(HC) 15
Yes
-
9
 K079038 TetO-wt/4C5G - operator library member pSB1AK3(HC) 15
Yes
-
10
 K079039 LexA 1 - operator library member pSB1AK3(HC) 16
Yes
11
 K079040 LexA 2 - opeartor library member pSB1A2(HC) 16
Yes
-
12
 K079041 Lambda OR1 - operator library membe pSB1A2(HC) 17
Yes
-
13
 K079042 Lambda OR2 - operator library member 17
-
14
 K079043 Lambda OR3 - operator library membe 17
-
15
 K079045 Lac operator library 78
-
16
 K079046 Tet operator library 61
-
17
 K079047 Lambda operator library 67
-
18
 K079048 LexA operator library 40
-
19
 K079049 GFP reporter protein under the control of the J23118 costitutive promoter and LexA 2 operator 981
-
20
 K079050 GFP reporter protein under the control of the J23100 costitutive promoter and LexA 2 operator 981
-
21
 K079023 O3Lambda
-
22
 K079024 O1LexA
-
23
 K079026 O2LexA
-
24
 K079029 O3LexA
-
25
 K079030 O2Lac-O1Lac
-
26
 K079031 O2Tet-O1Tet
-
27
 K079032 O2Lambda-O1Lambda
-
28
 O2LexA-O1LexA
-
29
 O3Lac-O2Lac-O1Lac
-
30
 O3Tet-O2Tet-O1Tet
-
31
 O3Lambda-O2Lambda-O1Lambda
-
32
 O3LexA-O2LexA-O1LexA
-
33
 O1Lac-RBS-LacI-T
-
34
 O2Lac-RBS-LacI-T
-
35
 O3Lac-RBS-LacI-T
-
36
 O1Lac-RBS-TetR-RBS-GFP-T
-
37
 O2Lac-RBS-TetR-RBS-GFP-T
-
38
 O3Lac-RBS-TetR-RBS-GFP-T
-


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