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Team:Bologna/Parts
From 2008.igem.org
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(āComposites) |
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| [http://partsregistry.org/Part:BBa_K079037 K079037] | | [http://partsregistry.org/Part:BBa_K079037 K079037] | ||
| TetO-4C - operator library member | | TetO-4C - operator library member | ||
| - | | | + | | |
| 15 | | 15 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
Revision as of 15:22, 27 October 2008
| HOME | TEAM | PROJECT | MODELING | WET-LAB | SOFTWARE | SUBMITTED PARTS | BIOSAFETY AND PROTOCOLS |
|---|
Composites
The table below shows all the composites that we have obtained to build up our final device. For each part you can find:
-in the second column the link to the Registry;
-in the third column the components for each part;
-in the fourth column the plasmid the part has been cloned in;
-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;
-in the sixth column cell vitality after transformation;
-in the seventh column fluo data for each part.
| NĀ° | Igem Code | Fragment | Plasmid | Length (bp) | Vitality | Fluo |
| | K0790015 | LacY transporter protein from E. coli | pSB1A2(HC) | 1254 | | |
| | K079017 | Lac symmetric - operator library member | 20 | | ||
| | K079018 | Lac 1 - operator library member | 21 | | | |
| | K079019 | Lac 2 - operator library member | 21 | | ||
| | K079020 | LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La | pSB1AK3 | 2165 | | |
| | K079021 | LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La | 2165 | | | |
| | K079036 | Tet O operator library member | 15 | | ||
| | K079037 | TetO-4C - operator library member | 15 | | | |
| | K079038 | TetO-wt/4C5G - operator library member | 15 | | | |
| | K079039 | LexA 1 - operator library member | 16 | | ||
| | K079040 | LexA 2 - opeartor library member | 16 | | | |
| | K079041 | Lambda OR1 - operator library member | 17 | | | |
| | K079042 | Lambda OR2 - operator library member | 17 | | | |
| | K079043 | Lambda OR3 - operator library member | 17 | | | |
| | K079045 | Lac operator library | 78 | | | |
| | K079046 | Tet operator library | 61 | | | |
| | K079047 | Lambda operator library | 67 | | | |
| | K079048 | LexA operator library | 40 | | | |
| | K079049 | GFP reporter protein under the control of the J23118 costitutive promoter and LexA 2 operator | pSB1A2(HC) | 981 | | |
| | K079050 | GFP reporter protein under the control of the J23100 costitutive promoter and LexA 2 operator | pSB1A2(HC) | 981 | | |
| | K079023 | LacI repressor under the control of the J23118 costitutive promoter and Lac 2 operator | pSB1A2(HC) | 1243 | | |
| | K079026 | GFP reporter protein regulated by J23118 costitutive promoter | pSB1A2(HC) | 2116 | | |
| | K079029 | 2501 | | | ||
| | K079030 | GFP reporter protein controlled by the BBa_23105 costitutive promoter | pSB1A2(HC) | 957 | | |
| | K079031 | GFP reporter protein under the control of the BBa_J23118 promoter | pSB1A2(HC) | 957 | | |
| | K079032 | GFP reporter protein under the control of the BBa_J23100 costitutive promoter | pSB1A2(HC) | 957 | | |
