Team:Bologna/Parts
From 2008.igem.org
(Difference between revisions)
(→Composites) |
(→Composites) |
||
Line 63: | Line 63: | ||
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079020 2165] | | [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079020 2165] | ||
| <center>Yes</center> | | <center>Yes</center> | ||
- | | | + | | <center>-</center> |
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
| <center>3</center> | | <center>3</center> | ||
Line 79: | Line 79: | ||
| 20 | | 20 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
- | | | + | | <center>-</center> |
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
| <center>5</center> | | <center>5</center> | ||
Line 95: | Line 95: | ||
| 21 | | 21 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
- | | | + | | <center>-</center> |
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
| <center>7</center> | | <center>7</center> | ||
Line 103: | Line 103: | ||
| 15 | | 15 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
- | | | + | | <center>-</center> |
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
| <center>8</center> | | <center>8</center> | ||
Line 127: | Line 127: | ||
| 16 | | 16 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
- | | | + | | <center>-</center> |
|- style="background:#f6efcd; color:black" | |- style="background:#f6efcd; color:black" | ||
| <center>11</center> | | <center>11</center> |
Revision as of 17:57, 27 October 2008
HOME | TEAM | PROJECT | MODELING | WET-LAB | SOFTWARE | SUBMITTED PARTS | BIOSAFETY AND PROTOCOLS |
---|
Composites
The table below shows all the composites that we have obtained to build up our final device. For each part you can find:
-in the second column the link to the Registry;
-in the third column the components for each part;
-in the fourth column the plasmid the part has been cloned in;
-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;
-in the sixth column cell vitality after transformation;
-in the seventh column fluo data for each part.
N° | Igem Code | Fragment | Plasmid | Length (bp) | Vitality | Fluo |
| K0790015 | LacY transporter protein from E. coli | pSB1AK3 (HC) | 1254 | | |
| K079020 | LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La | pSB1AK3 (HC) | 2165 | | |
| K079029 | 2501 | | | ||
| K079017 | Lac symmetric - operator library member | 20 | | | |
| K079018 | Lac 1 - operator library member | 21 | | | |
| K079019 | Lac 2 - operator library member | 21 | | | |
| K079036 | Tet O operator library member | 15 | | | |
| K079037 | TetO-4C - operator library member | 15 | | | |
| K079038 | TetO-wt/4C5G - operator library member | 15 | | | |
| K079039 | LexA 1 - operator library member | 16 | | | |
| K079040 | LexA 2 - opeartor library member | 16 | | | |
| K079041 | Lambda OR1 - operator library member | 17 | | | |
| K079042 | Lambda OR2 - operator library member | 17 | | | |
| K079043 | Lambda OR3 - operator library member | 17 | | | |
| K079045 | Lac operator library | 78 | | | |
| K079046 | Tet operator library | 61 | | | |
| K079047 | Lambda operator library | 67 | | | |
| K079048 | LexA operator library | 40 | | | |
| K079049 | GFP reporter protein under the control of the J23118 costitutive promoter and LexA 2 operator | pSB1A2 (HC) | 981 | | |
| K079050 | GFP reporter protein under the control of the J23100 costitutive promoter and LexA 2 operator | pSB1A2 (HC) | 981 | | |
| K079023 | LacI repressor under the control of the J23118 costitutive promoter and Lac 2 operator | pSB1A2 (HC) | 1243 | | |
| K079026 | GFP reporter protein regulated by J23118 costitutive promoter | pSB1A2 (HC) | 2116 | | |
| K079030 | GFP reporter protein controlled by the BBa_23105 costitutive promoter | pSB1A2 (HC) | 957 | | |
| K079031 | GFP reporter protein under the control of the BBa_J23118 promoter | pSB1A2 (HC) | 957 | | |
| K079032 | GFP reporter protein under the control of the BBa_J23100 costitutive promoter | pSB1A2 (HC) | 957 | | |