Team:Bologna/Parts

From 2008.igem.org

(Difference between revisions)
(→Composites)
(→Composites)
Line 59: Line 59:
| <center>2</center>
| <center>2</center>
| [http://partsregistry.org/Part:BBa_K079017 K079017]
| [http://partsregistry.org/Part:BBa_K079017 K079017]
-
| Lac symmetric - operator library membe
+
| Lac symmetric - operator library member
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)]
| 20
| 20
Line 67: Line 67:
| <center>3</center>
| <center>3</center>
| [http://partsregistry.org/Part:BBa_K079018 K079018]
| [http://partsregistry.org/Part:BBa_K079018 K079018]
-
| RBS-LacI-T
+
| Lac 1 - operator library member
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
| 21
| 21
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|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>4</center>
| <center>4</center>
-
| [http://partsregistry.org/Part:BBa_K079003 K079003]
+
| [http://partsregistry.org/Part:BBa_K079019 K079019]
-
| RBS-LacI-RBS-GFP-T
+
| Lac 2 - operator library member
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
-
| 2093
+
| 21
| <center>Yes</center>
| <center>Yes</center>
|
|
|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>5</center>
| <center>5</center>
-
| [http://partsregistry.org/Part:BBa_K079004 K079004]
+
| [http://partsregistry.org/Part:BBa_K079020 K079020]
-
| TetR-RBS-GFP-T
+
| LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)]
-
| 1607
+
| 2165
| <center>Yes</center>
| <center>Yes</center>
|  
|  
<!---|- style="background:#f6efcd; color:grey"
<!---|- style="background:#f6efcd; color:grey"
| <center>6</center>
| <center>6</center>
-
| [http://partsregistry.org/Part:BBa_K079005 K079005]
+
| [http://partsregistry.org/Part:BBa_K079021 K079021]
-
| TetR-T
+
| LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)]
-
| 822
+
| 2165
| <center>Yes</center>
| <center>Yes</center>
| <center>-</center> --->
| <center>-</center> --->
|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>7</center>
| <center>7</center>
-
| [http://partsregistry.org/Part:BBa_K079006 K079006]
+
| [http://partsregistry.org/Part:BBa_K079036 K079036]
-
| RBS-TetR-RBS-GFP-T
+
| Tet O operator library member
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
-
| 1625
+
| 15
| <center>Yes</center>
| <center>Yes</center>
|  
|  
|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>8</center>
| <center>8</center>
-
| [http://partsregistry.org/Part:BBa_K079007 K079007]
+
| [http://partsregistry.org/Part:BBa_K079037 K079037]
-
| RBS-TetR-T
+
| TetO-4C - operator library member
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)]
-
| 840
+
| 15
| <center>Yes</center>
| <center>Yes</center>
| <center>-</center>
| <center>-</center>
|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>9</center>
| <center>9</center>
-
| [http://partsregistry.org/Part:BBa_K079008 K079008]
+
| [http://partsregistry.org/Part:BBa_K079038 K079038]
-
| CI-T
+
| TetO-wt/4C5G - operator library member
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)]
-
| 912
+
| 15
| <center>Yes</center>
| <center>Yes</center>
| <center>-</center>
| <center>-</center>

Revision as of 10:09, 27 October 2008

Logo1a.gifTestata dx.jpg
HOME TEAM PROJECT MODELING WET-LAB SOFTWARE SUBMITTED PARTS BIOSAFETY AND PROTOCOLS


Composites

The table below shows all the composites that we have obtained to build up our final device. For each part you can find:

-in the second column the link to the Registry;

-in the third column the components for each part;

-in the fourth column the plasmid the part has been cloned in;

-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the sixth column cell vitality after transformation;

-in the seventh column fluo data for each part.


N° Igem Code Fragment Plasmid Length (bp) Vitality Fluo
1
 [http://partsregistry.org/Part:BBa_K079015 K0790015] LacY transporter protein from E. coli [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 1254
Yes
-
2
 [http://partsregistry.org/Part:BBa_K079017 K079017] Lac symmetric - operator library member [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 20
Yes
3
 [http://partsregistry.org/Part:BBa_K079018 K079018] Lac 1 - operator library member [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 21
Yes
-
4
 [http://partsregistry.org/Part:BBa_K079019 K079019] Lac 2 - operator library member [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 21
Yes
5
 [http://partsregistry.org/Part:BBa_K079020 K079020] LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 2165
Yes
7
 [http://partsregistry.org/Part:BBa_K079036 K079036] Tet O operator library member [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 15
Yes
8
 [http://partsregistry.org/Part:BBa_K079037 K079037] TetO-4C - operator library member [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 15
Yes
-
9
 [http://partsregistry.org/Part:BBa_K079038 K079038] TetO-wt/4C5G - operator library member [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 15
Yes
-
10
 [http://partsregistry.org/Part:BBa_K079009 K079009] CI-RBS-GFP-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 1697
Yes
11
 [http://partsregistry.org/Part:BBa_K079010 K079010] RBS-CI-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 930
Yes
-
12
 [http://partsregistry.org/Part:BBa_K079011 K079011] RBS-CI-RBS-GFP-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 1715
Yes
13
 O1Lac
-
14
 O2Lac
-
15
 O3Lac
-
16
 O1Tet
-
17
 O2Tet
-
18
 O3Tet
-
19
 O1Lambda
-
20
 O2Lambda
-
21
 O3Lambda
-
22
 O1LexA
-
23
 O2LexA
-
24
 O3LexA
-
25
 O2Lac-O1Lac
-
26
 O2Tet-O1Tet
-
27
 O2Lambda-O1Lambda
-
28
 O2LexA-O1LexA
-
29
 O3Lac-O2Lac-O1Lac
-
30
 O3Tet-O2Tet-O1Tet
-
31
 O3Lambda-O2Lambda-O1Lambda
-
32
 O3LexA-O2LexA-O1LexA
-
33
 O1Lac-RBS-LacI-T
-
34
 O2Lac-RBS-LacI-T
-
35
 O3Lac-RBS-LacI-T
-
36
 O1Lac-RBS-TetR-RBS-GFP-T
-
37
 O2Lac-RBS-TetR-RBS-GFP-T
-
38
 O3Lac-RBS-TetR-RBS-GFP-T
-


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