Revision as of 10:46, 3 October 2008

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Composites

The table below shows all the composites that we have obtained to build up our final device. For each part you can find:

-in the second column the link to the Registry;

-in the third column the components for each part;

-in the fourth column the plasmid the part has been cloned in;

-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the sixth column cell vitality after transformation;

-in the seventh column fluo data for each part.;

N°	Igem Code	Fragment	Plasmid	Length (bp)	Vitality	Fluo
1	[http://partsregistry.org/Part:BBa_K079000 K079000]	LacI-T		1290	Yes
2	[http://partsregistry.org/Part:BBa_K079001 K079001]	LacI-GFP		2075	Yes
3	[http://partsregistry.org/Part:BBa_K079002 K079002]	RBS-LacI-T		1308	Yes
4	[http://partsregistry.org/Part:BBa_K079003 K079003]	RBS-LacI-GFP		2093	Yes
5	[http://partsregistry.org/Part:BBa_K079004 K079004]	TetR-GFP		1607	Yes
6	[http://partsregistry.org/Part:BBa_K079005 K079005]	TetR-T		822	Yes
7	[http://partsregistry.org/Part:BBa_K079006 K079006]	RBS-TetR-GFP		1625	Yes
8	[http://partsregistry.org/Part:BBa_K079007 K079007]	RBS-TetR-T		840	Yes
9	[http://partsregistry.org/Part:BBa_K079008 K079008]	CI-T		912	Yes
10	[http://partsregistry.org/Part:BBa_K079009 K079009]	CI-GFP		1697	Yes
11	[http://partsregistry.org/Part:BBa_K0790010 K0790010]	RBS-CI-T		930	Yes
12	[http://partsregistry.org/Part:BBa_K0790011 K0790011]	RBS-CI-GFP		1715	Yes
13		O1Lac
14		O2Lac
15		O3Lac
16		O1Tet
17		O2Tet
18		O3Tet
19		O1Lambda
20		O2Lambda
21		O3Lambda
22		O1LexA
23		O2LexA
24		O3LexA
25		O2Lac-O1Lac
26		O2Tet-O1Tet
27		O2Lambda-O1Lambda
28		O2LexA-O1LexA
29		O3Lac-O2Lac-O1Lac
30		O3Tet-O2Tet-O1Tet
31		O3Lambda-O2Lambda-O1Lambda
32		O3LexA-O2LexA-O1LexA
33		O1Lac-RBS-LacI-T
34		O2Lac-RBS-LacI-T
35		O3Lac-RBS-LacI-T
36		O1Lac-RBS-TetR-GFP-T
37		O2Lac-RBS-TetR-GFP-T
38		O3Lac-RBS-TetR-GFP-T

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 | 1290
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 | 1308
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+| Yes
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 | 2093
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+| Yes
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+| Yes
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 | 822
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 | 1625
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+| Yes
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 | 840
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+| Yes
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 | 912
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+| Yes
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 | 1697
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+| Yes
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 | 930
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+| Yes
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 | 1715
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+| Yes
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 |- style="background:#f6efcd; color:grey"