Team:Bologna/Parts

From 2008.igem.org

(Difference between revisions)
(Composites)
(Composites)
Line 58: Line 58:
|- style="background:#f6efcd; color:grey"
|- style="background:#f6efcd; color:grey"
| <center>2</center>
| <center>2</center>
-
| [http://partsregistry.org/Part:BBa_K079001 K079001]
+
| [http://partsregistry.org/Part:BBa_K079017 K079017]
-
| LacI-RBS-GFP-T
+
| Lac symmetric - operator library membe
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)]
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)]
-
| 2075
+
| 20
| <center>Yes</center>
| <center>Yes</center>
|
|

Revision as of 10:02, 27 October 2008

Logo1a.gifTestata dx.jpg
HOME TEAM PROJECT MODELING WET-LAB SOFTWARE SUBMITTED PARTS BIOSAFETY AND PROTOCOLS


Composites

The table below shows all the composites that we have obtained to build up our final device. For each part you can find:

-in the second column the link to the Registry;

-in the third column the components for each part;

-in the fourth column the plasmid the part has been cloned in;

-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the sixth column cell vitality after transformation;

-in the seventh column fluo data for each part.


Igem Code Fragment Plasmid Length (bp) Vitality Fluo
1
[http://partsregistry.org/Part:BBa_K079015 K0790015] LacY transporter protein from E. coli [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 1254
Yes
-
2
[http://partsregistry.org/Part:BBa_K079017 K079017] Lac symmetric - operator library membe [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 20
Yes
3
[http://partsregistry.org/Part:BBa_K079002 K079002] RBS-LacI-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 1308
Yes
-
4
[http://partsregistry.org/Part:BBa_K079003 K079003] RBS-LacI-RBS-GFP-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 2093
Yes
5
[http://partsregistry.org/Part:BBa_K079004 K079004] TetR-RBS-GFP-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 1607
Yes
7
[http://partsregistry.org/Part:BBa_K079006 K079006] RBS-TetR-RBS-GFP-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 1625
Yes
8
[http://partsregistry.org/Part:BBa_K079007 K079007] RBS-TetR-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 840
Yes
-
9
[http://partsregistry.org/Part:BBa_K079008 K079008] CI-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 912
Yes
-
10
[http://partsregistry.org/Part:BBa_K079009 K079009] CI-RBS-GFP-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 1697
Yes
11
[http://partsregistry.org/Part:BBa_K079010 K079010] RBS-CI-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 930
Yes
-
12
[http://partsregistry.org/Part:BBa_K079011 K079011] RBS-CI-RBS-GFP-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 1715
Yes
13
O1Lac
-
14
O2Lac
-
15
O3Lac
-
16
O1Tet
-
17
O2Tet
-
18
O3Tet
-
19
O1Lambda
-
20
O2Lambda
-
21
O3Lambda
-
22
O1LexA
-
23
O2LexA
-
24
O3LexA
-
25
O2Lac-O1Lac
-
26
O2Tet-O1Tet
-
27
O2Lambda-O1Lambda
-
28
O2LexA-O1LexA
-
29
O3Lac-O2Lac-O1Lac
-
30
O3Tet-O2Tet-O1Tet
-
31
O3Lambda-O2Lambda-O1Lambda
-
32
O3LexA-O2LexA-O1LexA
-
33
O1Lac-RBS-LacI-T
-
34
O2Lac-RBS-LacI-T
-
35
O3Lac-RBS-LacI-T
-
36
O1Lac-RBS-TetR-RBS-GFP-T
-
37
O2Lac-RBS-TetR-RBS-GFP-T
-
38
O3Lac-RBS-TetR-RBS-GFP-T
-


Up