Team:Bologna/Parts
From 2008.igem.org
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| <center>11</center> | | <center>11</center> | ||
| [http://partsregistry.org/Part:BBa_K079040 K079040] | | [http://partsregistry.org/Part:BBa_K079040 K079040] | ||
- | | | + | | LexA 2 - opeartor library member |
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] | | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] | ||
- | | | + | | 16 |
| <center>Yes</center> | | <center>Yes</center> | ||
| <center>-</center> | | <center>-</center> | ||
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| <center>12</center> | | <center>12</center> | ||
| [http://partsregistry.org/Part:BBa_K079041 K079041] | | [http://partsregistry.org/Part:BBa_K079041 K079041] | ||
- | | | + | | Lambda OR1 - operator library membe |
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] | | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] | ||
- | | | + | | 17 |
| <center>Yes</center> | | <center>Yes</center> | ||
- | | | + | | <center>-</center> |
|- style="background:#f6efcd; color:grey" | |- style="background:#f6efcd; color:grey" | ||
| <center>13</center> | | <center>13</center> | ||
| [http://partsregistry.org/Part:BBa_K079042 K079042] | | [http://partsregistry.org/Part:BBa_K079042 K079042] | ||
- | | | + | | Lambda OR2 - operator library member |
- | + | ||
| | | | ||
+ | | 17 | ||
| <center></center> | | <center></center> | ||
| <center>-</center> | | <center>-</center> | ||
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| <center>14</center> | | <center>14</center> | ||
| [http://partsregistry.org/Part:BBa_K079043 K079043] | | [http://partsregistry.org/Part:BBa_K079043 K079043] | ||
- | | | + | | Lambda OR3 - operator library membe |
- | + | ||
| | | | ||
+ | | 17 | ||
| <center></center> | | <center></center> | ||
| <center>-</center> | | <center>-</center> | ||
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| <center>15</center> | | <center>15</center> | ||
| [http://partsregistry.org/Part:BBa_K079045 K079045] | | [http://partsregistry.org/Part:BBa_K079045 K079045] | ||
- | | | + | | Lac operator library |
- | + | ||
| | | | ||
+ | | 78 | ||
| <center></center> | | <center></center> | ||
| <center>-</center> | | <center>-</center> | ||
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| <center>16</center> | | <center>16</center> | ||
| [http://partsregistry.org/Part:BBa_K079046 K079046] | | [http://partsregistry.org/Part:BBa_K079046 K079046] | ||
- | | | + | | Tet operator library |
- | + | ||
| | | | ||
+ | | 61 | ||
| <center></center> | | <center></center> | ||
| <center>-</center> | | <center>-</center> | ||
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| <center>17</center> | | <center>17</center> | ||
| [http://partsregistry.org/Part:BBa_K079047 K079047] | | [http://partsregistry.org/Part:BBa_K079047 K079047] | ||
- | | | + | | Lambda operator library |
- | + | ||
| | | | ||
+ | | 67 | ||
| <center></center> | | <center></center> | ||
| <center>-</center> | | <center>-</center> | ||
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| <center>18</center> | | <center>18</center> | ||
| [http://partsregistry.org/Part:BBa_K079048 K079048] | | [http://partsregistry.org/Part:BBa_K079048 K079048] | ||
- | | | + | | LexA operator library |
- | + | ||
| | | | ||
+ | | 40 | ||
| <center></center> | | <center></center> | ||
| <center>-</center> | | <center>-</center> | ||
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| <center>19</center> | | <center>19</center> | ||
| [http://partsregistry.org/Part:BBa_K079049 K079049] | | [http://partsregistry.org/Part:BBa_K079049 K079049] | ||
- | | | + | | GFP reporter protein under the control of the J23118 costitutive promoter and LexA 2 operator |
- | + | ||
| | | | ||
+ | | 981 | ||
| <center></center> | | <center></center> | ||
| <center>-</center> | | <center>-</center> | ||
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| <center>20</center> | | <center>20</center> | ||
| [http://partsregistry.org/Part:BBa_K079050 K079050] | | [http://partsregistry.org/Part:BBa_K079050 K079050] | ||
- | | | + | | GFP reporter protein under the control of the J23100 costitutive promoter and LexA 2 operator |
- | + | ||
| | | | ||
+ | | 981 | ||
| <center></center> | | <center></center> | ||
| <center>-</center> | | <center>-</center> |
Revision as of 10:24, 27 October 2008
HOME | TEAM | PROJECT | MODELING | WET-LAB | SOFTWARE | SUBMITTED PARTS | BIOSAFETY AND PROTOCOLS |
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Composites
The table below shows all the composites that we have obtained to build up our final device. For each part you can find:
-in the second column the link to the Registry;
-in the third column the components for each part;
-in the fourth column the plasmid the part has been cloned in;
-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;
-in the sixth column cell vitality after transformation;
-in the seventh column fluo data for each part.
N° | Igem Code | Fragment | Plasmid | Length (bp) | Vitality | Fluo |
| [http://partsregistry.org/Part:BBa_K079015 K0790015] | LacY transporter protein from E. coli | [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] | 1254 | | |
| [http://partsregistry.org/Part:BBa_K079017 K079017] | Lac symmetric - operator library member | [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] | 20 | | |
| [http://partsregistry.org/Part:BBa_K079018 K079018] | Lac 1 - operator library member | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] | 21 | | |
| [http://partsregistry.org/Part:BBa_K079019 K079019] | Lac 2 - operator library member | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] | 21 | | |
| [http://partsregistry.org/Part:BBa_K079020 K079020] | LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La | [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] | 2165 | | |
| [http://partsregistry.org/Part:BBa_K079021 K079021] | LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La | [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] | 2165 | | |
| [http://partsregistry.org/Part:BBa_K079036 K079036] | Tet O operator library member | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] | 15 | | |
| [http://partsregistry.org/Part:BBa_K079037 K079037] | TetO-4C - operator library member | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] | 15 | | |
| [http://partsregistry.org/Part:BBa_K079038 K079038] | TetO-wt/4C5G - operator library member | [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] | 15 | | |
| [http://partsregistry.org/Part:BBa_K079039 K079039] | LexA 1 - operator library member | [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] | 16 | | |
| [http://partsregistry.org/Part:BBa_K079040 K079040] | LexA 2 - opeartor library member | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] | 16 | | |
| [http://partsregistry.org/Part:BBa_K079041 K079041] | Lambda OR1 - operator library membe | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] | 17 | | |
| [http://partsregistry.org/Part:BBa_K079042 K079042] | Lambda OR2 - operator library member | 17 | | | |
| [http://partsregistry.org/Part:BBa_K079043 K079043] | Lambda OR3 - operator library membe | 17 | | | |
| [http://partsregistry.org/Part:BBa_K079045 K079045] | Lac operator library | 78 | | | |
| [http://partsregistry.org/Part:BBa_K079046 K079046] | Tet operator library | 61 | | | |
| [http://partsregistry.org/Part:BBa_K079047 K079047] | Lambda operator library | 67 | | | |
| [http://partsregistry.org/Part:BBa_K079048 K079048] | LexA operator library | 40 | | | |
| [http://partsregistry.org/Part:BBa_K079049 K079049] | GFP reporter protein under the control of the J23118 costitutive promoter and LexA 2 operator | 981 | | | |
| [http://partsregistry.org/Part:BBa_K079050 K079050] | GFP reporter protein under the control of the J23100 costitutive promoter and LexA 2 operator | 981 | | | |
| [http://partsregistry.org/Part:BBa_K079023 K079023] | O3Lambda | | | ||
| [http://partsregistry.org/Part:BBa_K079024 K079024] | O1LexA | | | ||
| [http://partsregistry.org/Part:BBa_K079026 K079026] | O2LexA | | | ||
| [http://partsregistry.org/Part:BBa_K079029 K079029] | O3LexA | | | ||
| [http://partsregistry.org/Part:BBa_K079030 K079030] | O2Lac-O1Lac | | | ||
| [http://partsregistry.org/Part:BBa_K079031 K079031] | O2Tet-O1Tet | | | ||
| [http://partsregistry.org/Part:BBa_K079032 K079032] | O2Lambda-O1Lambda | | | ||
| O2LexA-O1LexA | | | |||
| O3Lac-O2Lac-O1Lac | | | |||
| O3Tet-O2Tet-O1Tet | | | |||
| O3Lambda-O2Lambda-O1Lambda | | | |||
| O3LexA-O2LexA-O1LexA | | | |||
| O1Lac-RBS-LacI-T | | | |||
| O2Lac-RBS-LacI-T | | | |||
| O3Lac-RBS-LacI-T | | | |||
| O1Lac-RBS-TetR-RBS-GFP-T | | | |||
| O2Lac-RBS-TetR-RBS-GFP-T | | | |||
| O3Lac-RBS-TetR-RBS-GFP-T | | |