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Composites

The table below shows all the composites that we have obtained to build up our final device. For each part you can find:

-in the second column the link to the Registry;

-in the third column the components for each part;

-in the fourth column the plasmid the part has been cloned in;

-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the sixth column cell vitality after transformation;

-in the seventh column fluo data for each part.

N°	Igem Code	Fragment	Plasmid	Length (bp)	Vitality	Fluo
1	K0790015	LacY transporter protein from E. coli	pSB1AK3(HC)	1254	Yes	-
2	K079017	Lac symmetric - operator library membe	pSB1AK3(HC)	20	Yes
3	K079018	RBS-LacI-T	pSB1A2(HC)	21	Yes	-
4	K079003	RBS-LacI-RBS-GFP-T	pSB1A2(HC)	2093	Yes
5	K079004	TetR-RBS-GFP-T	pSB1AK3(HC)	1607	Yes
7	K079006	RBS-TetR-RBS-GFP-T	pSB1A2(HC)	1625	Yes
8	K079007	RBS-TetR-T	pSB1A2(HC)	840	Yes	-
9	K079008	CI-T	pSB1AK3(HC)	912	Yes	-
10	K079009	CI-RBS-GFP-T	pSB1AK3(HC)	1697	Yes
11	K079010	RBS-CI-T	pSB1A2(HC)	930	Yes	-
12	K079011	RBS-CI-RBS-GFP-T	pSB1A2(HC)	1715	Yes
13		O1Lac				-
14		O2Lac				-
15		O3Lac				-
16		O1Tet				-
17		O2Tet				-
18		O3Tet				-
19		O1Lambda				-
20		O2Lambda				-
21		O3Lambda				-
22		O1LexA				-
23		O2LexA				-
24		O3LexA				-
25		O2Lac-O1Lac				-
26		O2Tet-O1Tet				-
27		O2Lambda-O1Lambda				-
28		O2LexA-O1LexA				-
29		O3Lac-O2Lac-O1Lac				-
30		O3Tet-O2Tet-O1Tet				-
31		O3Lambda-O2Lambda-O1Lambda				-
32		O3LexA-O2LexA-O1LexA				-
33		O1Lac-RBS-LacI-T				-
34		O2Lac-RBS-LacI-T				-
35		O3Lac-RBS-LacI-T				-
36		O1Lac-RBS-TetR-RBS-GFP-T				-
37		O2Lac-RBS-TetR-RBS-GFP-T				-
38		O3Lac-RBS-TetR-RBS-GFP-T				-

Team:Bologna/Parts