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HOME	TEAM	PROJECT	MODELING	WET-LAB	SOFTWARE	SUBMITTED PARTS	BIOSAFETY AND PROTOCOLS

Composites

The table below shows all the composites that we have obtained to build up our final device. For each part you can find:

-in the second column the link to the Registry;

-in the third column the components for each part;

-in the fourth column the plasmid the part has been cloned in;

-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the sixth column cell vitality after transformation;

-in the seventh column fluo data for each part.

N°	Igem Code	Fragment	Plasmid	Length (bp)	Vitality	Fluo
1	K0790015	LacY transporter protein from E. coli	pSB1AK3(HC)	1254	Yes	-
2	K079017	Lac symmetric - operator library member	pSB1AK3(HC)	20	Yes
3	K079018	Lac 1 - operator library member	pSB1A2(HC)	21	Yes	-
4	K079019	Lac 2 - operator library member	pSB1A2(HC)	21	Yes
5	K079020	LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La	pSB1AK3(HC)	2165	Yes
6	K079021	LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La	pSB1AK3(HC)	2165	Yes	-
7	K079036	Tet O operator library member	pSB1A2(HC)	15	Yes
8	K079037	TetO-4C - operator library member	pSB1A2(HC)	15	Yes	-
9	K079038	TetO-wt/4C5G - operator library member	pSB1AK3(HC)	15	Yes	-
10	K079039	LexA 1 - operator library member	pSB1AK3(HC)	16	Yes
11	K079040	LexA 2 - opeartor library member	pSB1A2(HC)	16	Yes	-
12	K079041	Lambda OR1 - operator library membe	pSB1A2(HC)	17	Yes	-
13	K079042	Lambda OR2 - operator library member		17		-
14	K079043	Lambda OR3 - operator library membe		17		-
15	K079045	Lac operator library		78		-
16	K079046	Tet operator library		61		-
17	K079047	Lambda operator library		67		-
18	K079048	LexA operator library		40		-
19	K079049	GFP reporter protein under the control of the J23118 costitutive promoter and LexA 2 operator		981		-
20	K079050	GFP reporter protein under the control of the J23100 costitutive promoter and LexA 2 operator		981		-
21	K079023	O3Lambda				-
22	K079024	O1LexA				-
23	K079026	O2LexA				-
24	K079029	O3LexA				-
25	K079030	O2Lac-O1Lac				-
26	K079031	O2Tet-O1Tet				-
27	K079032	O2Lambda-O1Lambda				-
28		O2LexA-O1LexA				-
29		O3Lac-O2Lac-O1Lac				-
30		O3Tet-O2Tet-O1Tet				-
31		O3Lambda-O2Lambda-O1Lambda				-
32		O3LexA-O2LexA-O1LexA				-
33		O1Lac-RBS-LacI-T				-
34		O2Lac-RBS-LacI-T				-
35		O3Lac-RBS-LacI-T				-
36		O1Lac-RBS-TetR-RBS-GFP-T				-
37		O2Lac-RBS-TetR-RBS-GFP-T				-
38		O3Lac-RBS-TetR-RBS-GFP-T				-

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Composites