Team:Cambridge/Signalling/Constructs

From 2008.igem.org

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(New page: Lowercase letters are biobrick ends, uppercase are the actual pieces. ==AgrA== <pre>atgaattcgcggccgcttctagagATGGAAATCGCACTGGCTACGGACAATCCATATGAGGTTCTGGAACAGGCTAAAAACATGAACGACATCGGCTGCTATT...)
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__NOEDITSECTION__

Revision as of 04:01, 30 October 2008

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Lowercase letters are biobrick ends, uppercase are the actual pieces.

Contents

AgrA

atgaattcgcggccgcttctagagATGGAAATCGCACTGGCTACGGACAATCCATATGAGGTTCTGGAACAGGCTAAAAACATGAACGACATCGGCTGCTATTTCCTGGATATTCAGCTGAGCACCGACATCAACGGTATCAAACTGGGCTCTGAAATCCGTAAACATGATCCGGTTGGTAACATTATTTTCGTGACTTCTCATTCTGAGCTGACCTATCTGACGTTCGTATACAAGGTTGCAGCCATGGATTTCATCTTTAAAGACGATCCGGCAGAGCTGCGTACGCGCATCATCGACTGTCTGGAAACTGCGCACACGCGCCTGCAACTGCTGTCCAAAGATAACTCCGTGGAAACCATTGAACTGAAACGTGGCAGCAACAGCGTTTACGTTCAGTACGATGATATCATGTTCTTCGAAAGCTCCACTAAGTCCCACCGTCTGATCGCGCATCTGGATAATCGTCAGATCGAGTTCTATGGCAACCTGAAAGAGCTGAGCCAGCTGGACGATCGCTTCTTCCGTTGTCATAACTCCTTCGTTGTTAACCGTCACAACATCGAATCCATCGACTCCAAAGAGCGTATTGTATACTTCAAGAACAAAGAACACTGTTATGCGTCTGTACGCAATGTCAAGAAAATCTAAtactagtagcggccgctgcaggcggc

AgrB

atgaattcgcggccgcttctagagATGAACTATTTTGACAACAAGATCGACCAATTTGCTACTTACCTGCAAAAACGCAACAACCTGGACCACATCCAGTTTCTGCAAGTTCGTCTGGGCATGCAGGTTCTGGCTAAAAACATCGGCAAACTGATCGTTATGTACACCATCGCCTATATTCTGAACATCTTCCTGTTTACCCTGATCACCAACCTGACCTTCTATCTGATTCGCCGTCATGCCCACGGCGCTCATGCACCTTCTTCCTTTTGGTGTTATGTAGAGAGCATTATTCTGTTTATCCTGCTGCCGCTGGTAATCGTTAACTTCCACATCAATTTCCTGATTATGATCATCCTGACTGTGATTTCCCTGGGTGTAATCTCTGTTTACGCTCCGGCTGCCACTAAGAAAAAGCCGATTCCGGTGCGCCTGATCAAACGTAAAAAGTACTACGCTATCATCGTTTCCCTGACCCTGTTCATTATCACCCTGATTATCAAAGAACCGTTTGCACAGTTCATCCAGCTGGGTATTATCATCGAAGCGATCACTCTGCTGCCGATCTTTTTCATCAAAGAGGATCTGAAATAAtactagtagcggccgctgcaggcggc

AgrC

atgaattcgcggccgcttctagagATGATTCTGATGTTCACCATTCCAGCAATTATTAGCGGTATTAAGTACAGCAAGCTGGATTATTTCTTCATCATCGTAATCTCTACCCTGTCCCTGTTCCTGTTCAAAATGTTCGATTCCGCGAGCCTGATTATCCTGACTTCCTTCATCATCATCATGTATTTCGTAAAAATCAAATGGTATAGCATCCTGCTGATCATGACCTCCCAGATTATCCTGTACTGTGCCAACTACATGTATATCGTTATCTACGCGTACATCACGAAAATCTCCGATTCTATCTTCGTTATCTTTCCGTCTTTCTTCGTGGTGTACGTTACGATCTCTATTCTGTTTTCCTATATTATCAATCGCGTTCTGAAAAAGATCTCTACTCCGTACCTGATTCTGAACAAAGGTTTTCTGATCGTCATCTCCACTATCCTGCTGCTGACCTTCTCTCTGTTCTTCTTTTACTCTCAGATCAACTCCGATGAGGCAAAAGTCATCCGTCAGTACAGCTTCATCTTTATCGGTATTACTATCTTCCTGAGCATTCTGACTTTCGTTATCAGCCAGTTCCTGCTGAAAGAAATGAAGTACAAGCGCAACCAAGAAGAAATCGAGACTTACTACGAGTACACCCTGAAGATTGAAGCGATCAACAACGAAATGCGTAAATTTCGTCACGACTACGTTAACATTCTGACCACCCTGAGCGAATACATCCGTGAAGACGACATGCCTGGCCTGCGTGATTATTTCAACAAAAACATTGTGCCGATGAAAGATAACCTGCAAATGAACGCTATTAAGCTGAACGGTATCGAAAACCTGAAAGTACGTGAGATCAAGGGTCTGATTACCGCTAAAATCCTGCGTGCGCAGGAAATGAACATCCCAATTAGCATCGAAATCCCAGACGAAGTTTCTAGCATTAACCTGAACATGATTGACCTGTCTCGTAGCATCGGCATCATCCTGGACAACGCTATCGAAGCTTCCACTGAAATCGACGACCCGATTATTCGCGTTGCTTTTATCGAGAGCGAAAACTCCGTGACCTTTATCGTGATGAACAAATGCGCGGACGATATCCCGCGTATTCACGAACTGTTCCAGGAATCTTTCAGCACCAAGGGTGAAGGTCGCGGCCTGGGCCTGTCCACCCTGAAAGAGATCGCGGATAACGCCGACAACGTTCTGCTGGACACTATTATCGAAAACGGTTTCTTCATTCAGAAAGTCGAAATCATCAATAACTAAtactagtagcggccgctgcaggcggc

AgrD

atgaattcgcggccgcttctagagATGAATACTCTGTTCAATCTGTTTTTCGATTTCATTACTGGCATCCTGAAGAACATCGGTAACATCGCAGCGTACAGCACCTGCGACTTCATCATGGATGAAGTAGAGGTCCCGAAAGAGCTGACCCAGCTGCATGAATAAtactagtagcggccgctgcaggcggc

pXyl Promoter

With BB sites: 163 Without: 115 

atgaattcgcggccgcttctagagGAAAAACTAAAAAAAATATTGAAAATACTGATGAGGTTATTTAAGATTAAAATAAGTTAGTTTGTTTGGGCAACAAACTAATGTGCAACTTACTTACAATATGACATAAAATGCAtactagtagcggccgctgcagatc

pSpac Promoter

With BB sites: 155 Without: 107 

atgaattcgcggccgcttctagagAGAACAACCTCTGCTAAAATTCCTGAAAAATTTTGCAAAAAGTTGTTGACTTTATCTACAAGGTGTGGCATAATGTGTGGAATTGTGAGCGGATAACAATTAAGCTTtactagtagcggccgctgcagata

pPac Promoter

With BB sites: 125 Without: 75 

atgaattcgcggccgcttctagagAAACGAGGTCATCATTTCCTTCCGAAAAAACGGTTGCATTTAAATCTTACATATGTAATACTTTCAAAGACTACAtactagtagcggccgctgcaggcggc

pUpp Promoter

With BB sites: 285 Without: 239 

gaattcgcggccgcttctagagATTTTTAAAGTATGTATACAAATGATGAATAAATTTTGGCGATATAATGAAGGATACAGCTCCCATAATTGGTAAAGATACTAGATAGATTCATCGTAAAATCATGATTTTGCCAAATTTGCCCTTGAATATTAGTAGCGTTTTCTTTACAATCGTAAATAGTGTAAAAAAGCGTGCAAACGCATGAATATCATCTAAAGGAGAGATTCACATGGGAAAACTGTATGTATTTGATCCTCtactagtagcggccgctgcagat

consensus B. subtilis RBS

With BB sites: 54 Without: 11 

gaattcgcggccgcttctagagAAAGGAGG TGTtactagtagcggccgctgcag

weak B. subtilis RBS

With BB sites: 54 Without: 11 

gaattcgcggccgcttctagagAGAGGTGG TGTtactagtagcggccgctgcag

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