Team:Chiba/Calendar-Home/16 September 2008

From 2008.igem.org

(Difference between revisions)
(New page: >Home | Notebook 15 September 2008 <|[[Team:Chiba/Calendar-Home/17 September 2008|> 17 September 2008...)
(→Team:Output)
Line 20: Line 20:
*[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
*[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
*[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
*[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
-
*[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)
+
*[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](RBS+cinR)
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
Line 28: Line 28:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA</td>
+
<td>DNA(μL)</td>
<td>3</td>
<td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ</td>
+
<td>EcoRⅠ(μL)</td>
<td>0.083</td>
<td>0.083</td>
</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ</td>
+
<td>PstⅠ(μL)</td>
<td>0.083</td>
<td>0.083</td>
</tr>
</tr>
-
<td>BSA(x10)</td>
+
<tr>
 +
<td>BSA(x10)(μL)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer3(x10)</td>
+
<td>Buffer3(x10)(μL)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(μL)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
Line 66: Line 67:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA</td>
+
<td>DNA(μL)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(μL)</td>
<td>2</td>
<td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
<td>12</td>
<td>12</td>
</tr>
</tr>
Line 94: Line 95:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA</td>
+
<td>DNA(μL)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Venus Fwd</td>
+
<td>Venus Fwd(μL)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Rev (VR)</td>
+
<td>Rev(VR)(μL)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Thermo pol Buffer</td>
+
<td>Thermo pol Buffer(μL)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dNTP</td>
+
<td>dNTP mix(μL)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Vent</td>
+
<td>Vent(μL)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(μL)</td>
<td>68</td>
<td>68</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
<td>100</td>
<td>100</td>
</tr>
</tr>
</table>
</table>

Revision as of 03:23, 25 October 2008

>Home | Notebook

15 September 2008 <|> 17 September 2008

Laboratory work

Team:Communication

(15/9)->

  1. strain
    Senders:JW1908
    Receiver:JW1908
  2. temparature
    37℃ and 30℃


Team:Output


Digestion test

Sample No. each
DNA(μL) 3
EcoRⅠ(μL) 0.083
PstⅠ(μL) 0.083
BSA(x10)(μL) 1
Buffer3(x10)(μL) 1
dH2O(μL) 5
TOTAL(μL) 10

-->37℃ 1hour

Gel check

Sample No. each
DNA(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12

Transformation

PCR

  • Venus YFP(ptet+LuxR+YFP)
Sample No. YFP
DNA(μL) 1
Venus Fwd(μL) 5
Rev(VR)(μL) 5
Thermo pol Buffer(μL) 10
dNTP mix(μL) 10
Vent(μL) 1
dH2O(μL) 68
TOTAL(μL) 100
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/16_September_2008"