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Team:Chiba/Calendar-Home/16 September 2008
From 2008.igem.org
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[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] | [[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] | ||
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*reaction temparaturewas 37°C and 30°C. | *reaction temparaturewas 37°C and 30°C. | ||
Revision as of 12:30, 26 October 2008
15 September 2008 <|> 17 September 2008
Laboratory work
Team:Communication
(15/9)-> time-delay test
- reaction temparaturewas 37°C and 30°C.
Team:Output
Digestion test
- BBa_R0040(ptet)+BBa_S03158(RBS+RhlR)(x2)
- BBa_R0040(ptet)+BBa_S03156(RBS+lasR)(x5)
- BBa_R0040(ptet)+Ba_S03160(RBS+cinR)(x2)
- BBa_S03158(RBS+RhlR)
- BBa_S03156(RBS+lasR)
- BBa_S03160(RBS+cinR)
| Sample No. | each |
| DNA(μL) | 3 |
| EcoRⅠ(μL) | 0.083 |
| PstⅠ(μL) | 0.083 |
| BSA(x10)(μL) | 1 |
| Buffer3(x10)(μL) | 1 |
| dH2O(μL) | 5 |
| TOTAL(μL) | 10 |
-->37℃ 1hour
| Sample No. | each |
| DNA(μL) | 10 |
| Loading Dye(μL) | 2 |
| TOTAL(μL) | 12 |
- BBa_J37019(LuxI+RBS+LuxR)->no colony
- BBa_J37033(RBS+LuxR)
- BBa_J37020(LuxI+LuxR+pLux+GFP)
- BBa_J37032(pLux+GFP)
- Venus YFP(ptet+LuxR+YFP)
| Sample No. | YFP |
| DNA(μL) | 1 |
| Venus Fwd(μL) | 5 |
| Rev(VR)(μL) | 5 |
| Thermo pol Buffer(μL) | 10 |
| dNTP mix(μL) | 10 |
| Vent(μL) | 1 |
| dH2O(μL) | 68 |
| TOTAL(μL) | 100 |
