Team:Chiba/Calendar-Home/16 September 2008
From 2008.igem.org
15 September 2008 <|> 17 September 2008
Laboratory work
Team:Communication
time-delay test with (15/9) cultures.
- reaction temparature was 37°C and 30°C.
- result
- Created Glycerol Stocks from (15/9) cultures.
Team:Output
Digestion test
- BBa_R0040(ptet)+BBa_S03158(RBS+RhlR)(×2)
- BBa_R0040(ptet)+BBa_S03156(RBS+lasR)(×5)
- BBa_R0040(ptet)+Ba_S03160(RBS+cinR)(×2)
- BBa_S03158(RBS+RhlR)
- BBa_S03156(RBS+lasR)
- BBa_S03160(RBS+cinR)
Sample No. | each |
DNA(μL) | 3 |
EcoRI(μL) | 0.083 |
PstI(μL) | 0.083 |
BSA(×10)(μL) | 1 |
Buffer3(×10)(μL) | 1 |
dH2O(μL) | 5 |
TOTAL(μL) | 10 |
-->37°C 1 hour
Sample No. | each |
DNA(μL) | 10 |
Loading Dye(μL) | 2 |
TOTAL(μL) | 12 |
- BBa_J37019(LuxI+RBS+LuxR)->no colony
- BBa_J37033(RBS+LuxR)
- BBa_J37020(LuxI+LuxR+pLux+GFP)
- BBa_J37032(pLux+GFP)
- Venus YFP(ptet+LuxR+YFP)
Sample No. | YFP |
DNA(μL) | 1 |
Venus Fwd(μL) | 5 |
Rev(VR)(μL) | 5 |
Thermo pol Buffer(μL) | 10 |
dNTP mix(μL) | 10 |
Vent(μL) | 1 |
dH2O(μL) | 68 |
TOTAL(μL) | 100 |