Team:Chiba/Calendar-Home/16 September 2008

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15 September 2008 <|> 17 September 2008

Laboratory work

Team:Communication

time-delay test with (15/9) cultures.

  • reaction temparature was 37°C and 30°C.
  • result
  • Created Glycerol Stocks from (15/9) cultures.



Team:Output


Digestion test

Sample No. each
DNA(μL) 3
EcoRI(μL) 0.083
PstI(μL) 0.083
BSA(×10)(μL) 1
Buffer3(×10)(μL) 1
dH2O(μL) 5
TOTAL(μL) 10

-->37°C 1 hour

Gel check

Sample No. each
DNA(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12

Transformation

PCR

Sample No. BBa_K084003
DNA(μL) 1
Venus Fwd(μL) 5
Rev(VR)(μL) 5
Thermo pol Buffer(μL) 10
dNTP mix(μL) 10
Vent(μL) 1
dH2O(μL) 68
TOTAL(μL) 100