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Team:Chiba/Calendar-Home/17 August 2008
From 2008.igem.org
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>DNA template</td> | + | <td>DNA template(μL)</td> |
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>FW primer</td> | + | <td>FW primer(μL)</td> |
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>VR primer</td> | + | <td>VR primer(μL)</td> |
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>dNTPmix</td> | + | <td>dNTPmix(μL)</td> |
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>thermo pol buffer</td> | + | <td>thermo pol buffer(μL)</td> |
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>DNA pol(VENT)</td> | + | <td>DNA pol(VENT)(μL)</td> |
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>dH<sub>2</sub>O</td> | + | <td>dH<sub>2</sub>O(μL)</td> |
<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td> | <td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td> | <td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
Latest revision as of 05:11, 30 October 2008
16 August 2008 <|> 18 August 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- Transformation
- competent cells : XL10G
- BBa_R0040(2008) --> No colonies on plates
- BBa_C0070(2008) --> No colonies on plates
- BBa_C0076(2008) --> No colonies on plates
- BBa_C0077(2008) --> No colonies on plates
- BBa_C0078(2008) --> No colonies on plates
- BBa_C0079(2008) --> No colonies on plates
Team:Output
- BBa_E0430(1)
- BBa_E0420(2)
- BBa_J06702(3)
- BBa_S01416(4)
- BBa_I730003(5)
- BBa_I730004(6)
- BBa_I763026(7)
- BBa_I763002(8)
| Sample No. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| DNA template(μL) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| FW primer(μL) | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| VR primer(μL) | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| dNTPmix(μL) | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| thermo pol buffer(μL) | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| DNA pol(VENT)(μL) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| dH2O(μL) | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 |
| TOTAL(μL) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
