Team:Chiba/Calendar-Home/18 September 2008

@@ Line 6: / Line 6: @@
 ===Team:Communication===
-[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
+*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
-*conditions
+**transformants
-#strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
+***[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
-#temparature<br>37℃ and 30℃
+***[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
+***[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
+**E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
+**temparature<br>37&deg;C  and 30&deg;C
 ===Team:Output===
-[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]] 1μL
+[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]] 1&mu;L
 ->left for:
-#1 hour at 37 degrees
+#1 hour at 37 &deg;C
-#30 minutes at 65 degrees.
+#30 minutes at 65 &deg;C
 [[Team:Chiba/protocol/transformation|Zymoclean]]
-->eluted with 25μL of NFW.
+->eluted with 25&mu;L of NFW.
 [[Team:Chiba/protocol/ligation/ligation|Ligation]]
 *vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
-*insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
+*insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119]
-*Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
+*insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 K084004]
+*insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 K084005]
+*insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 K084006]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
@@ Line 34: / Line 40: @@
 </tr>
 <tr>
-<td>DNA(vector)(μL)</td>
+<td>DNA(vector)(&mu;L)</td>
 <td>3.5</td><td>3.5</td>
 </tr>
 <tr>
-<td>DNA(insert)(μL)</td>
+<td>DNA(insert)(&mu;L)</td>
 <td>7.5</td><td>-</td>
 </tr>
 <tr>
-<td>Ligase(μL)</td>
+<td>Ligase(&mu;L)</td>
 <td>1</td><td>1</td>
 </tr>
 <tr>
-<td>Ligase Buffer(μL)</td>
+<td>Ligase Buffer(&mu;L)</td>
 <td>4</td><td>4</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O(μL)</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>4</td><td>4</td>
 </tr>
 <tr>
-<td>TOTAL(μL)</td>
+<td>TOTAL(&mu;L)</td>
 <td>20</td><td>20</td>
 </tr>
@@ Line 60: / Line 66: @@
 [[Team:Chiba/protocol/transformation|transformation]]
-*[http://partsregistry.org/Part:BBa_J54103 BBa_J54103](RBS+GFP)
+*[http://partsregistry.org/Part:BBa_J54103 BBa_J54103]
 *[http://partsregistry.org/Part:BBa_P2006 BBa_P2006]
 [[Team:Chiba/protocol/gelcheck|Gel check]]
-*Ligation product(Venus YFP)
+*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]
 *[http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
 *[http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
@@ Line 75: / Line 81: @@
 </tr>
 <tr>
-<td>DNA(μL)</td>
+<td>DNA(&mu;L)</td>
 <td>each</td>
 </tr>
 <tr>
-<td>Loading Dye(μL)</td>
+<td>Loading Dye(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O(μL)</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>4</td>
 </tr>
 <tr>
-<td>TOTAL(μL)</td>
+<td>TOTAL(&mu;L)</td>
 <td>6</td>
 </tr>
 </table>