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Team:Chiba/Calendar-Home/18 September 2008
From 2008.igem.org
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[[Team:Chiba/protocol/ligation/ligation|Ligation]] | [[Team:Chiba/protocol/ligation/ligation|Ligation]] | ||
*vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100] | *vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100] | ||
| - | *insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119] | + | *insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119] |
*Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR) | *Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR) | ||
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[[Team:Chiba/protocol/transformation|transformation]] | [[Team:Chiba/protocol/transformation|transformation]] | ||
| - | *[http://partsregistry.org/Part:BBa_J54103 BBa_J54103] | + | *[http://partsregistry.org/Part:BBa_J54103 BBa_J54103] |
*[http://partsregistry.org/Part:BBa_P2006 BBa_P2006] | *[http://partsregistry.org/Part:BBa_P2006 BBa_P2006] | ||
[[Team:Chiba/protocol/gelcheck|Gel check]] | [[Team:Chiba/protocol/gelcheck|Gel check]] | ||
| - | * | + | *[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_?K084003] |
*[http://partsregistry.org/Part:BBa_J37033 BBa_J37033] | *[http://partsregistry.org/Part:BBa_J37033 BBa_J37033] | ||
*[http://partsregistry.org/Part:BBa_J37020 BBa_J37020] | *[http://partsregistry.org/Part:BBa_J37020 BBa_J37020] | ||
Revision as of 22:37, 29 October 2008
17 September 2008 <|> 19 September 2008
Laboratory work
Team:Communication
- time-delay test
- transformants
- [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
- [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
- [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
- E.coli strain
Senders:XL10GOLD
Receiver:JW1908
- temparature
37°C and 30°C
- transformants
Team:Output
SAP 1μL
->left for:
- 1 hour at 37 °C
- 30 minutes at 65 °C
->eluted with 25μL of NFW.
- vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
- insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119]
- Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
| Sample No. | Vector&insert | Vector |
| DNA(vector)(μL) | 3.5 | 3.5 |
| DNA(insert)(μL) | 7.5 | - |
| Ligase(μL) | 1 | 1 |
| Ligase Buffer(μL) | 4 | 4 |
| dH2O(μL) | 4 | 4 |
| TOTAL(μL) | 20 | 20 |
- [http://partsregistry.org/Part:BBa_J54103 BBa_J54103]
- [http://partsregistry.org/Part:BBa_P2006 BBa_P2006]
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_?K084003]
- [http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
- [http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
- [http://partsregistry.org/Part:BBa_J37032 BBa_J37032]
| Sample No. | each |
| DNA(μL) | each |
| Loading Dye(μL) | 1 |
| dH2O(μL) | 4 |
| TOTAL(μL) | 6 |
