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Team:Chiba/Calendar-Home/18 September 2008
From 2008.igem.org
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**E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br> | **E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br> | ||
**temparature<br>37°C and 30°C | **temparature<br>37°C and 30°C | ||
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===Team:Output=== | ===Team:Output=== | ||
Latest revision as of 00:01, 30 October 2008
17 September 2008 <|> 19 September 2008
Laboratory work
Team:Communication
- time-delay test
- transformants
- E.coli strain
Senders:XL10GOLD
Receiver:JW1908
- temparature
37°C and 30°C
Team:Output
SAP 1μL
->left for:
- 1 hour at 37 °C
- 30 minutes at 65 °C
->eluted with 25μL of NFW.
- vector:BBa_I7100
- insert:BBa_S03119
- insert:K084004
- insert:K084005
- insert:K084006
| Sample No. | Vector&insert | Vector |
| DNA(vector)(μL) | 3.5 | 3.5 |
| DNA(insert)(μL) | 7.5 | - |
| Ligase(μL) | 1 | 1 |
| Ligase Buffer(μL) | 4 | 4 |
| dH2O(μL) | 4 | 4 |
| TOTAL(μL) | 20 | 20 |
| Sample No. | each |
| DNA(μL) | each |
| Loading Dye(μL) | 1 |
| dH2O(μL) | 4 |
| TOTAL(μL) | 6 |
