Team:Chiba/Calendar-Home/18 September 2008
From 2008.igem.org
17 September 2008 <|> 19 September 2008
Laboratory work
Team:Communication
- time-delay test
- transformants
- E.coli strain
Senders:XL10GOLD
Receiver:JW1908
- temparature
37°C and 30°C
Team:Output
SAP 1μL
->left for:
- 1 hour at 37 °C
- 30 minutes at 65 °C
->eluted with 25μL of NFW.
- vector:BBa_I7100
- insert:BBa_S03119
- insert:K084004
- insert:K084005
- insert:K084006
Sample No. | Vector&insert | Vector |
DNA(vector)(μL) | 3.5 | 3.5 |
DNA(insert)(μL) | 7.5 | - |
Ligase(μL) | 1 | 1 |
Ligase Buffer(μL) | 4 | 4 |
dH2O(μL) | 4 | 4 |
TOTAL(μL) | 20 | 20 |
Sample No. | each |
DNA(μL) | each |
Loading Dye(μL) | 1 |
dH2O(μL) | 4 |
TOTAL(μL) | 6 |