Team:Chiba/Calendar-Home/19 August 2008

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18 August 2008 <|> 20 August 2008

Contents

1 Laboratory work

Laboratory work

Team:Input

no work

Team:Communication

PCR

BBa_I9026(2007)
BBa_I9026(2006)
BBa_I9030(2007)
BBa_I9030(2006)
BBa_S3154(2007)
BBa_S3154(2006)
BBa_F2620(2007)･･･control

DNA Template	1
dNTP mix	10
Foward Primer	5
Reverse Primer	5
DNA polymerase VENT	1
Thermopol Buffer	5
dH₂O	28
TOTAL	50μL

95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃

--->Gel Check

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

BBa_I9026(2007) ->None
BBa_I9026(2006) ->None
BBa_I9030(2007) ->None
BBa_I9030(2006) ->None
BBa_S3154(2007) ->None
BBa_S3154(2006) ->None
BBa_F2620(2007)･･･control ->OK

Team:Output

no work

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