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Team:Chiba/Calendar-Home/19 September 2008
From 2008.igem.org
18 September 2008 <|> 20 September 2008
Laboratory work
Team:Communication
-->(?/9) Gel Check
| Sample Name | [http://partsregistry.org/Part:BBa_J04500 J04500] | [http://partsregistry.org/Part:BBa_R0010 R0010] | [http://partsregistry.org/Part:BBa_C0161 C0161] | Cin |
| Sample DNA(μL) | 30 | 30 | 90 | ? |
| Loading Dye(μL) | 6 | 6 | 18 | ? |
| TOTAL(μL) | 36 | 36 | 108 | 24 |
--->Gel Extract
(3 wells for 1 sample)
- note:ADB buffer添加後,left at 37°C,for 6 hours
- --->Zymo Clean
- elution
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500]-->27μL
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161]-->11μL
--->Gel Check
- --->Ligation
| Sample Name | insert+vector | insert | vector |
| [http://partsregistry.org/Part:BBa_C0161 C0161](μL) | 3 | 3 | - |
| [http://partsregistry.org/Part:BBa_J04500 J04500](μL) | 4.5 | - | 4.5 |
| ligase(μL) | 1 | 1 | 1 |
| Buffer(invitrogen)(μL) | 3 | 2 | 2 |
| NFW(μL) | 3.5 | 4 | 2.5 |
| TOTAL(μL) | 15 | 10 | 10 |
- --->Transformed above ligation onto a LB-amp plate.
- note
- competent cell:XL10GOLD
- DNA:1μL,SOC:120μL
--->(20/9)Mini prep
- time-delay test
- conditions
- strain
Senders:XL10GOLD
Receiver:JW1908
- temparature
30°C and Room Temparature
Team:Output
colony PCR
insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](×16)(maker:Kan)
insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 K084004](×5)(maker:Kan)
insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 K084005](×7)(maker:Kan)
insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 K084006](×5)(maker:Kan)
