Team:Chiba/Calendar-Home/20 August 2008

From 2008.igem.org

(Difference between revisions)
(Team:Input)
 
(11 intermediate revisions not shown)
Line 6: Line 6:
===Team:Input===
===Team:Input===
'''[[Team:Chiba/protocol/PCR|PCR]]'''
'''[[Team:Chiba/protocol/PCR|PCR]]'''
-
*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
+
*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)(1)
-
*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)
+
*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)(2)
-
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)
+
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)(3)
-
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)
+
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)(4)
-
*[http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)
+
*[http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)(5)
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
-
<td></td><td></td><td></td><td></td><td></td>
+
<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA tamplate</td>
+
<td>DNA tamplate(&mu;L)</td>
-
<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
+
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>FW primer</td>
+
<td>FW primer(&mu;L)</td>
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>VR primer</td>
+
<td>VR primer(&mu;L)</td>
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dNTPmix</td>
+
<td>dNTPmix(&mu;L)</td>
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>thermo pol buffer</td>
+
<td>thermo pol buffer(&mu;L)</td>
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA pol(VENT)</td>
+
<td>DNA pol(VENT)(&mu;L)</td>
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
</tr>
</tr>
Line 56: Line 56:
<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
-
<td></td><td></td><td></td><td></td><td></td>
+
<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
<td>Sample DNA</td>
<td>Sample DNA</td>
-
<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
+
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>loading dye</td>
+
<td>loading dye(&mu;L)</td>
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
<td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Total</td>
+
<td>Total(&mu;L)</td>
<td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
<td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
</tr>
</tr>
Line 77: Line 77:
</table>
</table>
-
-->どれもバンド出ず。
+
-->1,2,3,4,5:none
'''[[Team:Chiba/protocol/transformation|Transformation]]'''
'''[[Team:Chiba/protocol/transformation|Transformation]]'''
Line 86: Line 86:
*[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
*[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
-
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)
+
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)-->only one colony
-
 
+
-
-->BBa_J22141のみコロニーがほとんど生えていなかった。(1個のみ)
+
-
 
+
-
 
+
===Team:Communication===
===Team:Communication===
:(18/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
:(18/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
-
::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
+
::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
-
::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
+
::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
-
::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
+
::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
-
::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
+
::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
-
::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
+
::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
-
::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
+
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
-
::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
+
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
-
::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
+
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
-
::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
+
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
Line 119: Line 115:
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O</td>
<td>7</td>
<td>7</td>
</tr>
</tr>
Line 129: Line 125:
:From left;
:From left;
-
::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
+
::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
-
::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
+
::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
-
::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
+
::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> None
|}
|}
Line 149: Line 145:
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
Line 159: Line 155:
:From left;
:From left;
-
::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
+
::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
-
::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> OK
+
::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> OK
-
::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
+
::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
-
::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
+
::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007) -> OK
-
::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK
+
::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006) -> OK
|}
|}
Line 182: Line 178:
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O</td>
<td>7</td>
<td>7</td>
</tr>
</tr>
Line 192: Line 188:
:From left;<br>
:From left;<br>
-
::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK<br>
+
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
-
::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK<br>
+
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
-
::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK<br>
+
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
-
::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK<br>
+
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
|}
|}
--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
-
::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
+
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
-
::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
+
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
-
::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
+
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
-
::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
+
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
Line 211: Line 207:
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]</td>
+
<td>[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)</td>
<td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td>
<td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]</td>
+
<td>[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)</td>
<td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td>
<td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]</td>
+
<td>[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)</td>
<td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td>
<td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]</td>
+
<td>[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)</td>
<td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td>
<td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ</td>
+
<td>EcoRI</td>
<td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
<td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ</td>
+
<td>PstI</td>
<td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
<td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer EcoRⅠ</td>
+
<td>Buffer EcoRI</td>
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
</tr>
</tr>
Line 243: Line 239:
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O</td>
<td>4.9</td><td>4.9</td><td>4.9</td><td>4.9</td><td>4.8</td><td>4.8</td><td>4.8</td><td>4.8</td>
<td>4.9</td><td>4.9</td><td>4.9</td><td>4.9</td><td>4.8</td><td>4.8</td><td>4.8</td><td>4.8</td>
</tr>
</tr>
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+
<td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td>
</tr>
</tr>
</table>
</table>
Line 268: Line 264:
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O</td>
<td>7</td>
<td>7</td>
</tr>
</tr>
Line 278: Line 274:
:From left;
:From left;
:(Single Digestion 1~4)
:(Single Digestion 1~4)
-
::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
+
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
-
::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
+
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
-
::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
+
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
-
::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
+
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
:(Double Digestion 1~4)
:(Double Digestion 1~4)
-
::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
+
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
-
::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
+
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
-
::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
+
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
-
::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
+
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
|}
|}
-
 
-
 
-
 
-
 
===Team:Output===
===Team:Output===
[[Team:Chiba/protocol/transformation|Transformation]]
[[Team:Chiba/protocol/transformation|Transformation]]
-
*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](Luciferase Renilla)-->colony  
+
*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2007)-->colony  
-
*[http://partsregistry.org/Part:BBa_I712019 BBa_I712019](Luciferase Fire fly)-->no colony
+
*[http://partsregistry.org/Part:BBa_I712019 BBa_I712019](2008)-->no colony
-
*[http://partsregistry.org/Part:BBa_J33202 BBa_J33202](LacZ)-->colony
+
*[http://partsregistry.org/Part:BBa_J33202 BBa_J33202](2007)-->colony
-
*[http://partsregistry.org/Part:BBa_E2030 BBa_E2030](Venus YFP)-->not so good
+
*[http://partsregistry.org/Part:BBa_E2030 BBa_E2030](2007)-->not so good
-
*YFP(P-GEX)-->colony
+
*YFP(P-GEX)-->colony(Amp)
-
*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]-->colony
+
*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->colony
-
*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]-->colony
+
*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)-->colony
-
 
+
-
<br>Luciferase(J52008:2007,2008 I712019:2008)(Amp)
+
-
<br>Lac Z(J33202:2007,2008)(Amp)
+
-
<br>Venus(E2030:2007,2008)(Kam)
+
-
<br>Craig Venus(Amp?Kan?)
+
-
<br>T9002(Amp)
+
-
<br>F2620(Amp)
+
-
 
+
-
 
+
-
 
+
-
<br>luciferase,LacZ,Venus
+
-
<br>competent cell  50μl
+
-
<br>DNA  1μl
+
-
<br>soc  200μl
+
-
<br>plating 250μl
+
-
 
+
-
<br>Craig Venus,T9002,F2620
+
-
<br>competent cell  30μl
+
-
<br>DNA  1μl
+
-
<br>soc  120μl
+
-
<br>plating 20μl
+
-
 
+
-
<br>result
+
-
:成功→Luciferase(J52008:2007),Lac Z(J33202:2007),Craig Venus,T9002,F2620
+
-
:失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008)
+

Latest revision as of 05:57, 30 October 2008

>Home | Notebook

19 August 2008 <|> 21 August 2008

Contents

Laboratory work

Team:Input

PCR

  • [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)(1)
  • [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)(2)
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)(3)
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)(4)
  • [http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)(5)
Sample No. 12345
DNA tamplate(μL) 11111
FW primer(μL) 55555
VR primer(μL) 55555
dNTPmix(μL) 1010101010
thermo pol buffer(μL) 1010101010
DNA pol(VENT)(μL) 11111
dH2O(μL) 6868686868
TOTAL(μL) 100100100100100

-->Gel Check


Sample No. 12345
Sample DNA 11111
loading dye(μL) 11111
dH2O(μL) 44444
Total(μL) 66666

-->1,2,3,4,5:none

Transformation

  • [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
  • [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
  • [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
  • [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
  • [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)-->only one colony

Team:Communication

(18/8)--->Mini prep
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)


--->Gel Check
Chiba-0820-3.JPG
Sample DNA 3
Loading Dye 2
dH2O 7
TOTAL 12μL
From left;
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> None


Chiba-0820-4.JPG
Sample DNA 10
Loading Dye 4
dH2O 10
TOTAL 24μL
From left;
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006) -> OK



Chiba-0820.JPG
Sample DNA 3
Loading Dye 2
dH2O 7
TOTAL 12μL
From left;
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK


--->Digestion test

  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
Sample No 12345678
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) 3---3---
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -3---3--
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) --3---3-
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) ---3---3
EcoRI 0.10.10.10.10.10.10.10.1
PstI ----0.10.10.10.1
Buffer EcoRI 11111111
BSA 11111111
dH2O 4.94.94.94.94.84.84.84.8
TOTAL 10μL10μL10μL10μL10μL10μL10μL10μL


--->Gel Check
Chiba-0820-2.JPG
Sample DNA 3
Loading Dye 2
dH2O 7
TOTAL 12μL
From left;
(Single Digestion 1~4)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
(Double Digestion 1~4)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK

Team:Output

Transformation

  • [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2007)-->colony
  • [http://partsregistry.org/Part:BBa_I712019 BBa_I712019](2008)-->no colony
  • [http://partsregistry.org/Part:BBa_J33202 BBa_J33202](2007)-->colony
  • [http://partsregistry.org/Part:BBa_E2030 BBa_E2030](2007)-->not so good
  • YFP(P-GEX)-->colony(Amp)
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->colony
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)-->colony