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Team:Chiba/Calendar-Home/22 August 2008
From 2008.igem.org
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===Team:Output=== | ===Team:Output=== | ||
[[Team:Chiba/protocol/DNA_Purification/sigma|Mini prep]] | [[Team:Chiba/protocol/DNA_Purification/sigma|Mini prep]] | ||
| - | *[http://partsregistry.org/Part:BBa_J52008 BBa_J52008] | + | *[http://partsregistry.org/Part:BBa_J52008 BBa_J52008] |
*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] | *[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] | ||
*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002] | *[http://partsregistry.org/Part:BBa_T9002 BBa_T9002] | ||
| - | *[http://partsregistry.org/Part:BBa_J33202 BBa_J33202] | + | *[http://partsregistry.org/Part:BBa_J33202 BBa_J33202] |
*PUC19 | *PUC19 | ||
*PGEX VENUS YFP(GST fusion) | *PGEX VENUS YFP(GST fusion) | ||
Revision as of 15:04, 24 October 2008
21 August 2008 <|> 23 August 2008
Contents |
Laboratory work
Team:Input
Mini prep: Minipreped the cultures(<--21/8).
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
- [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
- [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)
insert check:Digestion
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
- [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
- [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
混ぜ表
| Sample No. | 1-4 | 1-6 |
| dH2O | 14 | 25 |
| 10×BSA | 7 | 10 |
| 10×NE | 7 | 10 |
| EcoRI | 3.5 | 5 |
| PstI | 3.5 | - |
UV照射実験(液体編)(~24)
-Ptrc-LuxR-Plux-cI-colE1-Amp-,-PcI-GFP-p15a-Cm-(BW)の2plasmidを使用。
グリストをつついて2ml培養×2(LB-Amp,Cm)(LB-Amp,cm,AHL100nM)
37℃で、12h培養後、濁ったらそのまま小さなプレートにLB培地ごと移してUVを照射する。UVの波長は254nm,UVランプまでの距離は8cm。
UVを照射して、15min,4h,8h,12h,たったら、両方のプレートからLB培地ごと20μlずつ採取しそれぞれ105希釈し、そこから20μlをそれぞれ(LB-Amp,Cm)(LB-Amp,cm,AHL100nM)のプレートに撒いた。
37℃で12h培養した後のコロニー数
| AHLなし | AHL100nM | |
| 15min | 136 | 0? |
| 4h | 40 | 31 |
| 8h | 10 | 0 |
| 12h | 0 | 0 |
いづれのプレートもAHLが入っていないものはGFPが確認できたが、AHL100nMのものは確認できなかった。
Team:Communication
- Transformation
- competent cells : XL10G
- [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
- [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
- (Locate & 2 Punch ~iyama ver.~)
- [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0077 BBa_C0077](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2008) -> No colonies on plates
--->(24/8)Mini prep
Team:Output
- [http://partsregistry.org/Part:BBa_J52008 BBa_J52008]
- [http://partsregistry.org/Part:BBa_F2620 BBa_F2620]
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]
- [http://partsregistry.org/Part:BBa_J33202 BBa_J33202]
- PUC19
- PGEX VENUS YFP(GST fusion)
- PGEX VENUS YFP(GST fusion)
