Team:Chiba/Calendar-Home/23 August 2008

From 2008.igem.org

(Difference between revisions)
(Team:Communication)
(Team:Communication)
Line 40: Line 40:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA</td>
+
<td>DNA(&mu;L)</td>
<td>3</td><td>1</td><td>3</td><td>3</td>
<td>3</td><td>1</td><td>3</td><td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRI</td>
+
<td>EcoRI(&mu;L)td>
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>PstI</td>
+
<td>PstI(&mu;L)</td>
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer EcoRI</td>
+
<td>Buffer EcoRI(&mu;L)</td>
<td>-</td><td>-</td><td>0.9</td><td>0.8</td>
<td>-</td><td>-</td><td>0.9</td><td>0.8</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BSA</td>
+
<td>BSA(&mu;L)</td>
<td>-</td><td>-</td><td>1</td><td>1</td>
<td>-</td><td>-</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>-</td><td>-</td><td>5</td><td>5</td>
<td>-</td><td>-</td><td>5</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>3μl</td><td>1μl</td><td>10μl</td><td>10μl</td>
+
<td>3</td><td>1</td><td>10</td><td>10</td>
</tr>
</tr>
</table>
</table>
Line 85: Line 85:
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>1</td><td>1</td>
<td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>2</td><td>4</td>
<td>2</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>6μl</td><td>6μl</td>
+
<td>6</td><td>6</td>
</tr>
</tr>
</table>
</table>
Line 120: Line 120:
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>2</td>
<td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>12μl</td>
+
<td>12</td>
</tr>
</tr>
</table>
</table>
Line 165: Line 165:
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>3</td><td>3</td><td>3</td>
<td>3</td><td>3</td><td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRI</td>
+
<td>EcoRI(&mu;L)</td>
<td>-</td><td>0.1</td><td>0.1</td>
<td>-</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SpeI</td>
+
<td>SpeI(&mu;L)</td>
<td>-</td><td>-</td><td>0.1</td>
<td>-</td><td>-</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRI Buffer</td>
+
<td>EcoRI Buffer(&mu;L)</td>
<td>-</td><td>0.9</td><td>0.8</td>
<td>-</td><td>0.9</td><td>0.8</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BSA</td>
+
<td>BSA(&mu;L)</td>
<td>-</td><td>1</td><td>1</td>
<td>-</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>-</td><td>5</td><td>5</td>
<td>-</td><td>5</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>3</td><td>10</td><td>10</td>
<td>3</td><td>10</td><td>10</td>
</tr>
</tr>
Line 209: Line 209:
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>1</td><td>2</td><td>2</td>
<td>1</td><td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>2</td><td>-</td><td>-</td>
<td>2</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>6μl</td><td>12μl</td><td>12μl</td>
+
<td>6</td><td>12</td><td>12</td>
</tr>
</tr>
</table>
</table>

Revision as of 06:01, 30 October 2008

>Home | Notebook

22 August 2008 <|> 24 August 2008

Contents

Meeting with UmeG

the ppt for our weekly meeting(japanese)

  1. Team:Input
  2. Team:Communication
  3. team:Output


Laboratory work

Team:Input

no work

Team:Communication

(21/8)--->Mini prep
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)


--->Digestion test
  1. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  2. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
  3. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  4. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  5. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  6. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
  7. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)


Sample No. 1- 671 - 71 - 7
DNA(μL) 3133
EcoRI(μL)td> --0.10.1
PstI(μL) --0.10.1
Buffer EcoRI(μL) --0.90.8
BSA(μL) --11
dH2O(μL) --55
TOTAL(μL) 311010


--->Gel Check
Chiba-0823-1.JPG
Sample No. 1~67
Sample DNA 31
Loading Dye(μL) 11
dH2O(μL) 24
TOTAL(μL) 66
From left; Sanple No. 1~7
  1. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  2. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
  3. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  4. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  5. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  6. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
  7. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)



Chiba-0823-2.JPG
Sample No. 8~21
Sample DNA(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12
From left;
(Single Digestion) Sanple No. 8~14
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
  • [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
(Double Digestion) Sample No. 15~21
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
  • [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK?



Digestion test
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007:old-sample)
Sample No. 123
Sample DNA(μL) 333
EcoRI(μL) -0.10.1
SpeI(μL) --0.1
EcoRI Buffer(μL) -0.90.8
BSA(μL) -11
dH2O(μL) -55
TOTAL(μL) 31010


--->Gel Check

Chiba-0823-3.JPG
Sample No. 123
Sample DNA 31010
Loading Dye(μL) 122
dH2O(μL) 2--
TOTAL(μL) 61212
From left;
  1. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Single Digestion -> OK
  3. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Double Digestion -> Bad

Team:Output

no work