Team:Chiba/Calendar-Home/23 August 2008

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22 August 2008 <|> 24 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(21/8)--->Mini prep
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]


--->Digestion test
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]


Sample No 1 2 3 4 5 6 7 8 9101112131415161718192021
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] 3------3------3------
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -3------3------3-----
BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] --3------3------3----
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] ---3------3------3---
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] ----3------3------3--
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -----3------3------3-
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] ------1------3------3
EcoRⅠ -------0.10.10.10.10.10.10.10.10.10.10.10.10.10.1
PstⅠ --------------0.10.10.10.10.10.10.1
Buffer EcoRⅠ -------0.90.90.90.90.90.90.90.80.80.80.80.80.80.8
BSA -------11111111111111
dH2O -------55555555555555
TOTAL 33333311010101010101010101010101010


--->Gel Check
Chiba-0823-1.JPG
Sample No 1~67
Sample DNA 31
Loading Dye 11
dH2O 24
TOTAL 66
From left; Sanple No. 1~7
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]



Chiba-0823-2.JPG
Sample No 8~21
Sample DNA 10
Loading Dye 2
TOTAL 12
From left;
(Single Digestion) Sanple No. 8~14
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
(Double Digestion) Sample No. 15~21
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK?



Digestion test
BBa_J04500(2007:old-sample)[http://partsregistry.org/Part:BBa_J04500]
Sample No 123
Sample DNA 333
EcoRⅠ -0.10.1
SpeⅠ --0.1
EcoRⅠ Buffer -0.90.8
BSA -11
dH2O -55
TOTAL 31010


--->Gel Check

Chiba-0823-3.JPG
Sample No 123
Sample DNA 31010
Loading Dye 122
dH2O 2--
TOTAL 61212
From left;
  1. BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
  2. BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Single Digestion -> OK
  3. BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion -> Bad


Team:Output

no work