Team:Chiba/Calendar-Home/23 August 2008

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22 August 2008 <|> 24 August 2008

Laboratory work

Team:Input

no work

Team:Communication

(21/8)--->Mini prep

• [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
• [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
• [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
• [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
• [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
• [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)

--->Digestion test

1. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
2. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
3. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
4. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
5. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
6. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
7. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)

Sample No.	1- 6	7	1 - 7	1 - 7
DNA	3	1	3	3
EcoRⅠ	-	-	0.1	0.1
PstⅠ	-	-	0.1	0.1
Buffer EcoRⅠ	-	-	0.9	0.8
BSA	-	-	1	1
dH2O	-	-	5	5
TOTAL	3	1	10	10

--->Gel Check

Sample No 1~6 7 Sample DNA 3 1 Loading Dye 1 1 dH2O 2 4 TOTAL 6 6 From left; Sanple No. 1~7 BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]

Sample No 8~21 Sample DNA 10 Loading Dye 2 TOTAL 12 From left; (Single Digestion) Sanple No. 8~14 BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK (Double Digestion) Sample No. 15~21 BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK?

Digestion test

BBa_J04500(2007:old-sample)[http://partsregistry.org/Part:BBa_J04500]

Sample No	1	2	3
Sample DNA	3	3	3
EcoRⅠ	-	0.1	0.1
SpeⅠ	-	-	0.1
EcoRⅠ Buffer	-	0.9	0.8
BSA	-	1	1
dH2O	-	5	5
TOTAL	3	10	10

--->Gel Check

Sample No 1 2 3 Sample DNA 3 10 10 Loading Dye 1 2 2 dH2O 2 - - TOTAL 6 12 12 From left; BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Single Digestion -> OK BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion -> Bad

Team:Output

no work

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/23_August_2008"

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