Team:Chiba/Calendar-Home/24 August 2008
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===Team:Output=== | ===Team:Output=== | ||
[[Team:Chiba/protocol/transformation|Transformation]] | [[Team:Chiba/protocol/transformation|Transformation]] | ||
- | *[http://partsregistry.org/Part:BBa_J63001 BBa_J63001] | + | *[http://partsregistry.org/Part:BBa_J63001 BBa_J63001]-->colony |
[[Team:Chiba/protocol/digestion|Digestion]] | [[Team:Chiba/protocol/digestion|Digestion]] |
Revision as of 15:04, 24 October 2008
23 August 2008 <|> 25 August 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- (22/8)--->Mini prep
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
- [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
- [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)
- --->Digestion test
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
- [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
- [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)
Sample No. | 5 | 6 | 7 | 8 |
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) | 1 | - | - | - |
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) | - | 1 | - | - |
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) | - | - | 1 | - |
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) | - | - | - | 1 |
EcoRⅠ | 0.1 | 0.1 | 0.1 | 0.1 |
Buffer 4 | 0.9 | 0.9 | 0.9 | 0.9 |
dH2O | 8 | 8 | 8 | 8 |
TOTAL | 10 | 10 | 10 | 10 |
- --->Gel Check
|
Team:Output
- [http://partsregistry.org/Part:BBa_J63001 BBa_J63001]-->colony
- [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)①
- [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)②
- Menbren Venus YFP③
Sample No. | ① | ② | ③ |
DNA template | 3 | 3 | 3 |
Buffer3 | 1 | 1 | 1 |
dH2O | 6 | 6 | 6 |
EcoRⅠ | 0.5 | 0.5 | 0.5 |
TOTAL | 10 | 10 | 10 |
-->37℃ 30min
Sample No. | ①(afetr digestion) | ②(after digestion) | ③(afetr digestion) |
DNA tamplate | 10 | 10 | 10 |
loading Dye | 2 | 2 | 2 |
TOTAL | 12 | 12 | 12 |
Sample No. | ① | ② | ③ |
DNA tamplate | 2 | 2 | 2 |
loading Dye | 1 | 1 | 1 |
dH2O | 3 | 3 | 3 |
TOTAL | 6 | 6 | 6 |
-->135V 30min