Team:Chiba/Calendar-Home/24 August 2008

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Revision as of 06:04, 30 October 2008

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23 August 2008 <|> 25 August 2008

Laboratory work

Team:Input

no work

Team:Communication

(22/8)--->Mini prep

BBa_J04500(2007)
BBa_J04500(2006)
BBa_J13002(2007)
BBa_J13002(2006)

--->Digestion test

BBa_J04500(2007)
BBa_J04500(2006)
BBa_J13002(2007)
BBa_J13002(2006)

Sample No.	5	6	7	8
BBa_J04500(2007)(μL)	1	-	-	-
BBa_J04500(2006)(μL)	-	1	-	-
BBa_J13002(2007)(μL)	-	-	1	-
BBa_J13002(2007)(μL)	-	-	-	1
EcoRI(μL)	0.1	0.1	0.1	0.1
Buffer 4(μL)	0.9	0.9	0.9	0.9
dH₂O(μL)	8	8	8	8
TOTAL(μL)	10	10	10	10

--->Gel Check

Sample No.	1~8
Sample DNA(μL)	1
Loading Dye(μL)	1
dH₂O(μL)	4
TOTAL(μL)	6

From left;

BBa_J04500(2007) -> ?
BBa_J04500(2006) -> OK
BBa_J13002(2007) -> OK
BBa_J13002(2006) -> OK
BBa_J04500(2007) -> OK
BBa_J04500(2006) -> OK
BBa_J13002(2007) -> OK
BBa_J13002(2006) -> None?

Sample No.	5~8
Sample DNA(μL)	9
Loading Dye(μL)	2
dH₂O(μL)	1
TOTAL(μL)	12

From the left; （single digestuion）

5, BBa_J04500(2007) -> OK

6, BBa_J04500(2006) -> OK

7, BBa_J13002(2007) -> 3400bp:Bad, 2200bp:OK

8, BBa_J13002(2006) -> OK

Team:Output

Transformation

BBa_J63001-->colony

Digestion

BBa_F2620(1)
BBa_F2620(2)
Menbren Venus YFP(3)

Sample No.	1	2	3
DNA template	3	3	3
Buffer3	1	1	1
dH₂O	6	6	6
EcoRI	0.5	0.5	0.5
TOTAL	10μl	10μl	10μl

-->37°C 30min

Sample No.	1(afetr digestion)	2(after digestion)	3(afetr digestion)
DNA tamplate	10	10	10
loading Dye	2	2	2
TOTAL	12μl	12μl	12μl

Sample No.	1	2	3
DNA tamplate	2	2	2
loading Dye	1	1	1
dH₂O	3	3	3
TOTAL	6μl	6μl	6μl

-->135V 30min

@@ Line 28: / Line 28: @@
 </tr>
 <tr>
-<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)</td>
+<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)(&mu;L)</td>
 <td>1</td><td>-</td><td>-</td><td>-</td>
 </tr>
 <tr>
-<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)</td>
+<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)(&mu;L)</td>
 <td>-</td><td>1</td><td>-</td><td>-</td>
 </tr><tr>
-<td>[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)</td>
+<td>[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)(&mu;L)</td>
 <td>-</td><td>-</td><td>1</td><td>-</td>
 </tr><tr>
-<td>[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)</td>
+<td>[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)(&mu;L)</td>
 <td>-</td><td>-</td><td>-</td><td>1</td>
 </tr>
 <tr>
-<td>EcoRI</td>
+<td>EcoRI(&mu;L)</td>
 <td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
 </tr>
 <tr>
-<td>Buffer 4</td>
+<td>Buffer 4(&mu;L)</td>
 <td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>8</td><td>8</td><td>8</td><td>8</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
-<td>10μl</td><td>10μl</td><td>10μl</td><td>10μl</td>
+<td>10</td><td>10</td><td>10</td><td>10</td>
 </tr>
 </table>
@@ Line 71: / Line 71: @@
 </tr>
 <tr>
-<td>Sample DNA</td>
+<td>Sample DNA(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>4</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
-<td>6μl</td>
+<td>6</td>
 </tr>
 </table>
@@ Line 106: / Line 106: @@
 </tr>
 <tr>
-<td>Sample DNA</td>
+<td>Sample DNA(&mu;L)</td>
 <td>9</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(&mu;L)</td>
 <td>2</td></tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
-<td>12μl</td>
+<td>12</td>
 </tr>
 </table>